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Method for applying chromatographic column temperature equalizer

A technology of chromatographic columns and homogenizers, applied in the field of temperature homogenizers, can solve problems such as instability, inconsistent working temperature of chromatographic columns, affecting the accuracy and repeatability of multi-dimensional high-performance liquid chromatography, and achieve the effect of reasonable structure

Inactive Publication Date: 2015-04-15
NANTONG UNIVERSITY
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Problems solved by technology

The above-mentioned factors that cause the inconsistent, unstable and uneven working temperature of the chromatographic column can affect the accuracy and repeatability of the multidimensional HPLC analysis

Method used

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  • Method for applying chromatographic column temperature equalizer
  • Method for applying chromatographic column temperature equalizer

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Embodiment Construction

[0030] A temperature homogenizer integrating multiple chromatographic columns with different lengths, including an upper metal heat conduction block and a lower metal heat conduction block, the upper metal heat conduction block and the lower metal heat conduction block are linked by hinges, the upper metal heat conduction block, the lower metal heat conduction block A plurality of upper semicircular chromatographic column slots and a plurality of lower semicircular chromatographic column slots are respectively set on the sheet metal heat conduction block, and the number of the upper semicircular chromatographic column slots and the lower semicircular chromatographic column slots are the same, and the positions are set correspondingly ;In each upper semicircular chromatographic column groove and lower semicircular chromatographic column groove, according to the length of the chromatographic column, place one or more sets of metal sleeves, each set of metal sleeves consists of a p...

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Abstract

The invention discloses a method for applying a chromatographic column temperature equalizer. The method comprises the following steps: connecting chromatographic columns; according to the length of the chromatographic columns, using multiple sets of chromatographic-column semicircular metal sleeves to cohere the chromatographic columns; installing the chromatographic columns with different lengths into the temperature equalizer integrating multiple chromatographic columns with different lengths; putting the temperature equalizer integrating multiple chromatographic columns into a chromatographic column thermostat of an efficient liquid chromatograph; starting up for detection and the like. According to the method, the heat conduction efficiency of the thermostat of the efficient liquid chromatograph can be increased, the heat balance time is shortened, and the coincidence, stability and uniformity of the working temperature of the chromatographic columns are kept.

Description

[0001] This application is a divisional application with application number: 201210397641.6, application date: 2012.10.19, and name "integrated temperature homogenizer with multiple chromatographic columns of different lengths". technical field [0002] The invention relates to a temperature homogenizer integrated with a plurality of chromatographic columns with different lengths used in a chromatographic column constant temperature box of a high performance liquid chromatograph. Background technique [0003] Multidimensional high performance liquid chromatography uses two or more chromatographic columns with different properties to separate components in complex samples. In the detection of multi-dimensional high-performance liquid chromatography, the sample is pre-separated in the first-dimensional chromatographic column, and then all or part of the pre-separated components are selectively transferred to another or more different types of chromatographic columns. column fo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/54
Inventor 邱一华彭聿平邱奥望刘展韩新华
Owner NANTONG UNIVERSITY
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