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Method for applying chromatographic column temperature equalizer

A chromatographic column and homogenizer technology, applied in the field of temperature homogenizers, can solve the problems of instability, non-uniformity, affecting the accuracy and repeatability of multi-dimensional high performance liquid chromatography analysis, and achieve the effect of reasonable structure

Inactive Publication Date: 2014-06-18
NANTONG UNIVERSITY
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Problems solved by technology

The above-mentioned factors that cause the inconsistent, unstable and uneven working temperature of the chromatographic column can affect the accuracy and repeatability of the multidimensional HPLC analysis

Method used

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  • Method for applying chromatographic column temperature equalizer
  • Method for applying chromatographic column temperature equalizer

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Embodiment Construction

[0030] A temperature homogenizer integrating a plurality of chromatographic columns of different lengths, comprising an upper metal heat conduction block, a lower metal heat conduction block, the upper metal heat conduction block and the lower metal heat conduction block are linked by hinges, the upper metal heat conduction block and the lower metal heat conduction block are connected by hinges. A plurality of upper semicircular chromatographic column grooves and a plurality of lower semicircular chromatographic column grooves are respectively set on the sheet metal heat conducting block, and the upper semicircular chromatographic column grooves and the lower semicircular chromatographic column grooves have the same number and corresponding positions. ;In each upper semicircular chromatographic column groove and lower semicircular chromatographic column groove, one or more sets of metal sleeves are respectively placed according to the length of the chromatographic column, and ea...

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Abstract

The invention discloses a method for applying a chromatographic column temperature equalizer. The method comprises the following steps: connecting chromatographic columns; according to the length of the chromatographic columns, using multiple sets of chromatographic-column semicircular metal sleeves to cohere the chromatographic columns; installing the chromatographic columns with different lengths into the temperature equalizer integrating multiple chromatographic columns with different lengths; putting the temperature equalizer integrating multiple chromatographic columns into a chromatographic column thermostat of an efficient liquid chromatograph; starting up for detection and the like. According to the method, the heat conduction efficiency of the thermostat of the efficient liquid chromatograph can be increased, the heat balance time is shortened, and the coincidence, stability and uniformity of the working temperature of the chromatographic columns are kept.

Description

[0001] This application is a divisional application with application number: 201210397641.6, application date: 2012.10.19, titled "temperature homogenizer integrating multiple chromatographic columns of different lengths". technical field [0002] The invention relates to a temperature homogenizer integrated with a plurality of chromatographic columns of different lengths used in a chromatographic column thermostat of a high performance liquid chromatograph. Background technique [0003] Multidimensional high performance liquid chromatography uses two or more chromatographic columns with different properties to separate components in complex samples. In the detection of multi-dimensional high performance liquid chromatography, the sample is pre-separated in the first-dimension chromatographic column, and then all or part of the pre-separated components are selectively transferred to another or more different types of chromatographs. further separation in the column. Multidi...

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Application Information

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IPC IPC(8): G01N30/54
Inventor 邱一华彭聿平邱奥望刘展韩新华
Owner NANTONG UNIVERSITY
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