Rice os05g26890.1 protein, gene encoding the protein and its application
A gene and protein technology, applied in rice small-grain dwarf gene and its application field
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Embodiment 1
[0026] Example 1 Gene mapping of rice small grain dwarf gene Os05g26890.1
[0027] Cross the rice small-grain dwarf mutant ‘2338 (小)’ with the normal indica variety ‘9311’, and all F1 plants have normal seed sizes. Investigating the seed size of the F2 segregation group plants, it was found that the separation ratio of the normal seed plants and the small seed plants was in line with 3:1, indicating that this trait is controlled by a pair of recessive genes.
[0028] Using molecular markers showing polymorphism between '2338 (small)' and '9311' to analyze normal DNA pools and mutant DNA pools, it was found that RM18451 on chromosome 5 was linked to the target trait, and this marker pair was further used. Linkage analysis of 100 recessive single plants in the 2338(小)' / '9311'F2 population revealed that they were closely linked to the mutant phenotype, so the target gene was initially located on chromosome 5 of rice.
[0029] figure 1 Comparing the grain and ear size of mutant ‘2338(小)...
Embodiment 2
[0034] Example 2 Identification of Candidate Genes Leading to the '2338 (Small)' Mutant Phenotype
[0035] Using http: / / rice.plantbiology.msu.edu / index.shtml, gene prediction was performed on the interval near RM18451, and an open reading frame Os05g26890.1 encoding the alpha subunit of G protein was found. The mutation table was reported in the literature. The type is similar to '2338 (small)', so the inventors designed 4 primers (Table 1), and amplified the full sequence of Os05g26890.1 from '9311' and '2338 (small)'. Sequencing revealed that the gene sequence of Os05g26890.1 was mutated in '2338 (small)', and the 11th exon of the gene sequence of Os05g26890.1 was missing 3 nucleotides: AAG. Therefore, Os05g26890.1 was determined to be a candidate gene that caused the '2338 (small)' mutant phenotype, and it was named as the rice small grain dwarf gene Os05g26890.1. Its nucleotide sequence is shown in SEQ ID No. 2, which encodes the amino acid of the protein. The sequence is sh...
Embodiment 3
[0038] Example 3 Cultivation of temperature-sensitive sterile lines carrying recessive small grain genes
[0039] The operation process of cultivating the temperature-sensitive sterile line carrying the small rice dwarf gene Os05g26890.1 described in Example 1 is as follows:
[0040] In 2004, a mutant ‘2338 (small)’ was obtained from Muyun Base in Changsha, with a thousand-grain weight of 14.5g (the original parent was 30g / 1000 grains). In 2006, the stable '2338(小)' was cross-bred with the dual-purpose nuclear sterile line'Xiangling 628S'. In 2010, the stable genetic '2338S' dual-purpose nuclear sterile line was obtained in the Muyun base of Changsha. The 1,000-grain weight is 15g. On the other hand, the 1,000-grain weight of general sterile rice lines'Y58S' (trade name) and'Guangzhan 63S' (trade name) is about 25g. Assuming that '2338S' (thousand-grain weight 15g small-grain) sterile line and'Guangzhan 63S' (thousand-grain weight 25g large-grain) sterile line are produced, the F...
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