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Ultrafast diagnostic tissue preparations as an alternative to frozen sections

A technology of solid tissues and mixtures, which is applied in the preparation of test samples, determination/inspection of microorganisms, microorganisms, etc., and can solve problems such as inconsistencies and delays

Active Publication Date: 2017-09-22
UNIV OF MIAMI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The absence of histological artifacts is an improvement over conventional histological detection of frozen sections, which is known in the art to be expected to produce inconsistent and delayed diagnoses

Method used

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  • Ultrafast diagnostic tissue preparations as an alternative to frozen sections
  • Ultrafast diagnostic tissue preparations as an alternative to frozen sections
  • Ultrafast diagnostic tissue preparations as an alternative to frozen sections

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0193] Fresh (i.e., not frozen and not pre-fixed) tissue is cut to a generally uniform thickness of about 0.6 mm (e.g., from about 0.4 mm to about 0.8 mm) to provide one or more tissue samples for processing . Hardening preferably begins when solid tissue is sectioned by contacting fresh tissue with a chemical mixture, which may or may not be the same as that used for processing (see below) during cutting. According to the new method, residual tissues from multiple different and diseased skin, lung, spleen, liver, uterus, placenta, intestine, ovary, carcinoma (e.g. breast, kidney, testis), sarcoma and leiomyoma were prepared Slice forever.

[0194] Tissue specimens were processed in a chemical mixture containing about 90% (v / v) acetone, about 10% (v / v) mineral oil, and about 0.1% (v / v) dimethylsulfoxide (DMSO). The two main components were mixed in a volume of 3.8 L, to which 5 ml of DMSO was added. The tissue specimen is incubated in the chemical mixture at about 50° C. fo...

Embodiment 2

[0197] One embodiment of the invention can be used (in figure 2 shown in ) was tissue processed in the following manner. In the microwave unit, a chemical mixture and microwave energy are brought into contact with the tissue specimen to harden the tissue specimen in a chamber whose interior is shaped like a whispering gallery. A radiation source M provides microwave energy in the hardening module. Stirring in the microwave unit can be provided by aeration (pump BP). The chemical mixture can be transferred between its storage and its chambers using lines regulated by valves, level monitors and pump LP. Then, in a vacuum unit, the hardened cast specimen is brought into contact with the molten matrix and thermal energy under vacuum (pump AP) in a chamber for infiltration. The Joule heat source H provides the heat source in the infiltration module. Agitation in the vacuum unit can be provided by P / V circulation using the same pump AP which also transfers the molten substrate ...

Embodiment 3

[0207] Example 3: Cutting of Solid Tissue

[0208] Another embodiment of the present invention can be used in the following manner (in Figure 4 to Figure 9 shown in ) to perform tissue dissection. The cutting system includes a sterilizable base 20 , a tissue support 30 and a tissue retainer 40 . Tissue support 30 and tissue anchor 40 may be fabricated from a resilient plastic material such as acrylic. Each has a generally planar roughened surface (eg, a plurality of barbs, spikes or serrations having an area similar to that of fresh tissue); during cutting, both roughened surfaces come into contact with the tissue. The retainer has a handle on the top and a rough surface on the bottom. Alternatively, fingers can be used in place of the anchors to apply light pressure to the solid tissue and push it against the support (not shown).

[0209] The tissue holder 30 can be snugly engaged with the receiving hole 24 by being pushed in with the fingers, so that the upper surface o...

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Abstract

Improved methods and systems for processing said entity tissue. The method can be performed manually or automatically. The system has features such as (i) a cutting module, in which fresh tissue is sectioned to prepare a tissue sample, (ii) a hardening module, which hardens a tissue sample, (iii) an infiltration module, which infiltrates a hardened tissue sample, and (iv) a package An embedding block, which embeds a hardened and infiltrated tissue specimen. Fresh (ie, non-fixed or frozen) tissue is cut to approximately 0.6 mm, which is dissected to diagnose disease or evaluate surgical treatment. Hardening of fresh tissue is preferably initiated but not completed during cutting by contact with the chemical mixture. Dry ice, a thermoelectric device, or a gas condenser are preferably used to cool the metal mold containing the embedded specimen. They were sectioned and then examined microscopically as an alternative to histological examination of frozen sections to avoid the known problems of inconsistency and delayed diagnosis.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of US Patent Application No. 61 / 540947, filed September 29, 2011. technical field [0003] The present invention relates to chemical methods and mechanical devices for diagnostic tissue preparations. Fresh (i.e., not fixed or frozen) samples of solid tissue excised from the patient during surgery can be prepared (i.e., reprocessed after cutting) as an alternative to frozen sections for histological examination, and do not suffer from postoperative The artifact of the author. These methods and these systems enable intraoperative diagnosis by histological examination of paraffin sections, which avoids the artifacts encountered when examining frozen sections. Background technique [0004] Methods and systems for tissue processing have been described (see WO 99 / 09390, WO 01 / 44783, WO 01 / 44784 and WO 2005 / 40763). They require a mixture of at least ketones and alcohols for chemical pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/31G01N33/50C12M1/42C12N13/00C12N5/07C12Q1/02
CPCG01N1/06G01N1/36G01N1/30G01N1/312
Inventor 阿佐里徳·R·莫拉莱斯
Owner UNIV OF MIAMI
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