Function and application of centrifugal force and shear stress response gene 1 in fatty liver and diabetes

A technology of shear stress and centrifugal force, applied in gene therapy, metabolic diseases, pharmaceutical formulations, etc., can solve problems such as increasing blood volume, affecting insulin function, and liver damage

Active Publication Date: 2016-08-24
武汉惠康达科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] 4) Insulin sensitizers work by activating PPAR-, and their biggest side effects are liver damage and increased blood volume, thus increasing the burden on the heart
Therefore, it is speculated that the expression of RECS1 gene may affect the function of insulin by regulating TNF-α signal.

Method used

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  • Function and application of centrifugal force and shear stress response gene 1 in fatty liver and diabetes
  • Function and application of centrifugal force and shear stress response gene 1 in fatty liver and diabetes
  • Function and application of centrifugal force and shear stress response gene 1 in fatty liver and diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] [Example 1] Mouse fatty liver and diabetes model (DIO) obtained

[0051] 1. Grouping of experimental animals: 8-week-old, male, WT mice and RECS1 KO mice were selected and fed with two special diets, D12942 high-fat diet (HFD) and D12450B low-fat diet (Normal chow, NC) respectively , that is WT NC group, KO NC group, WT HFD group, KO HFD group a total of 4 groups.

[0052] 2. Operation process of high-fat feed induction model:

[0053] Using WT and KO mice, the DIO model was established, and the phenotype correlation analysis was carried out to clarify that the RECS1 gene plays an important role in the pathogenesis of metabolic diseases. 8-week-old, male, WT mice and RECS1 KO mice were selected and fed with two special diets, D12942 high-fat diet (Highfat diet, HFD) and D12450B low-fat diet (Normal chow, NC), respectively, that is, WTNC group, KO There are 4 groups including NC group, WT HFD group and KO HFD group. The food intake of the mice was recorded in detail e...

Embodiment 2

[0054] [Example 2] Determination of body weight, blood glucose and insulin levels in fatty liver and diabetes model (DIO) mice

[0055] 1. Fasting body weight of mice, food intake detection, orbital sinus blood collection.

[0056] 1) Weight detection

[0057] ① Fasting: At 8:00 am, the mice to be tested are fasted (without water), and the experimental operation is started at 2:00 pm.

[0058] ② Weighing: Weigh at 0 weeks, 4 weeks, 8 weeks, 12 weeks, 16 weeks, 20 weeks, and 24 weeks, put a small plastic bucket on a dynamic electronic balance, grab the mouse, put it into the weighing In the small bucket, measure the body weight and record the data. Feed amount detection: After the weighing operation is completed, add feed to the mice, and record the amount of feed for the mice on the dynamic electronic balance.

[0059] 2) Blood was collected from the orbital sinus after ether inhalation anesthesia, and the serum was separated.

[0060] ① Anesthesia: Put the cotton ball dip...

Embodiment 3

[0093] [Example 3] Intraperitoneal glucose tolerance test (IPGTT)

[0094] On the 22nd week of the experiment, the intraperitoneal glucose injection test (IPGTT) was performed to evaluate the glucose tolerance of the mice.

[0095] ① Before measuring blood glucose, measure the fasting body weight of the mouse, and calculate the injection volume of glucose based on 10 μL / g.

[0096] ② First test the fasting blood sugar at 0 minutes before the glucose injection, and inject the glucose solution intraperitoneally quickly after the test is completed.

[0097] ③ Operation method of intraperitoneal injection: (1) Fix the mouse; grab the mouse, grasp the tail of the mouse with the little finger and ring finger of the left hand, and grasp the neck of the mouse with the other three fingers, so that the head of the mouse is downward, and the The abdomen of the mouse is fully exposed. (2) Needle positioning and injection: Insert the needle from the side of the abdomen and hold the syrin...

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Abstract

The invention discloses functions and applications of RECS1 in the fatty liver and the diabetes mellitus, and belongs to the field of functions and applications of genes. An RECS1 gene knockout mouse and a wild type C57 mouse are used as experimental subjects, through a model of the fatty liver and the diabetes mellitus which are induced by high-fat diets, the result shows that compared with the wild type C57 mouse, glycometabolism of the RECS1 gene knockout mouse is disorderly, fatty liver lesion is severe, and lipid accumulation is remarkably increased. It explains that the RECS1 has the adjustment and control capacity for maintaining the steady state of glycometabolism and has the liver lipid removal capacity and fatty liver forming resistance. Thus, the functions of the RECS1 in the fatty liver and the diabetes mellitus are found and the functions are mainly reflected in the effects on alleviating fatty liver and diabetes mellitus. For the functions of the RECS1, the RECS1 can be used for preparing medicine for treating the fatty liver and the diabetes mellitus.

Description

technical field [0001] The invention belongs to the field of gene function and application, in particular to the function and application of a centrifugal force and shear stress response gene 1 (responsive to centrifugal force and shear stress gene 1, RECS1) in fatty liver and diabetes. Background technique [0002] Diabetes is a chronic metabolic disease that seriously endangers human health. It is estimated that the number of patients will increase to 439 million by 2030, which means that 7.7% of adults aged 20-79 in the world will be diabetics. In the past two or three decades, the number of people with diabetes has doubled, and it is an extremely serious threat to human health around the world. At present, the incidence of type 2 diabetes and pre-type 2 diabetes among children, adolescents and young adults is increasing sharply, which also means that the incidence of diabetes will expand in the future, and the difficulty of prevention and treatment will increase greatly ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61P1/16A61P3/10
Inventor 李红良赵光年汪涛杜成
Owner 武汉惠康达科技有限公司
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