Anti-oxidative cattle frozen semen diluent and preparation method thereof

Abstract

The invention discloses anti-oxidative cattle frozen semen diluent. The anti-oxidative cattle frozen semen diluent is composed of the following raw materials by mass: 20-26 parts of trihydroxy methyl-aminomethane, 10-15 parts of trisodium citrate, 7-15 parts of fructose, 700-800 parts of distilled water, 230-260 parts of fresh yolk, 0.125-0.375 part of sodium selenite, 2-32 parts of Vitamin E, 90-95 parts of glycerol, 1-2 parts of penicillin potassium for injection (specification of 1.6 million units/g) and 1-2 parts of streptomycin sulfate for injection (specification of 1.0 million units/g). The preparation method of the anti-oxidative cattle frozen semen diluent comprises the steps of preparing a basic buffer solution, a basic diluent and a semen diluent; then transferring the prepared semen diluent to a condition of 4 DEG C, and balancing for 5-20 min to obtain the anti-oxidative cattle frozen semen diluent. Compared with a conventional anti-oxidative cattle frozen semen diluent, the anti-oxidative cattle frozen semen diluent provided by the invention increases semen motility rate by 24.56%, increases 4 h semen motility rate by 12.39%, increases semen perforatorium integrity rate by 38.72%, increases GSH-PX activity by 260%, and reduces semen aberration rate by 5.49%.

Description

technical field [0001] The invention belongs to the production technology of animal frozen semen, and in particular relates to a preparation method of frozen semen diluent added with trace elements selenium and vitamin E. Background technique [0002] The reproduction of cattle is an important link in the production process of cattle breeding. The increase in the number and quality of cattle must be realized through the process of reproduction. Artificial insemination and embryo transfer technology are two major technological revolutions in the production of cattle breeding, which can give full play to the reproductive potential of excellent breeding bulls and cows, showing a good application prospect. The premise of artificial insemination and embryo transfer technology is the application of bovine semen preservation technology. The success of cryopreservation of semen is a major innovation in artificial insemination technology. It not only solves the problem of long-term ...

AI Technical Summary

Problems solved by technology

Artificial insemination is a mature technology, but when using frozen semen for artificial insemination, its fertilization effect is lower than that of using fresh semen. Quality change is an important factor affecting the success of artificial insemination

[0004] The existing frozen semen dilutions are usually added with diluents, nutrients, antibacterial, antifreeze, anti-shock and buffer protection agents, etc., while the substances that can resist oxidative damage during the freezing and thawing of semen are limited to vitamins. E, vitamin C and glutathione and other substances, due to insufficient anti-oxidative damage ability, reduce the survival rate, survival rate and acrosome integrity rate after thawing, and reduce the quality of semen