22 results about "Potassium penicillin g" patented technology

A method for separating amoxicillin and phenylacetic acid from reaction solution in one-step enzymatic synthesis of amoxicillin is provided. The method employs immobilized penicillin acylase mutant to catalyze the one-step synthesis of amoxicillin from penicillin potassium, and develops a separation process for the resulting reaction mixture. The technical scheme mainly comprises: Firstly separating the immobilized penicillin acylase mutant from the reaction solution through filtration; subsequently isolating amoxicillin via crystallization; followed by separating and recovering phenylacetic acid through toluene extraction and back extraction. This separation method enables rapid and efficient isolation of amoxicillin with high production yield, achieving an average crystallization rate of 93.22%. Concurrently, it demonstrates effective separation and recovery of phenylacetic acid while allowing recyclable use of the toluene extractant.

The invention discloses a bacteriostatic composition which comprises a synergist and a bacteriostatic agent, the synergist is ethyl phenylacetate or a derivative thereof, and the bacteriostatic agent is selected from hydrogen peroxide, beta-lactam antibiotics, macrolide antibiotics and aminoglycoside antibiotics. The synergist disclosed by the invention is wide in synergistic effect spectrum range, and has a remarkable synergistic bacteriostatic effect when being combined with hydrogen peroxide, amoxicillin, ampicillin, benzylpenicillin potassium, erythromycin, azithromycin and amikacin; the antibacterial effect is weak when the compound is independently used, but the virulence of staphylococcus aureus can be remarkably reduced, the formation of hemolysin, staphylococcus flavin, enterotoxin and a biofilm can be inhibited, and meanwhile, the selective pressure on bacteria is favorably reduced due to the relatively low antibacterial activity, so that the occurrence of drug resistance is delayed; the method is suitable for various application scenes such as food processing industry.

The invention discloses soluble powder for preventing and treating chicken necrotic enteritis, and belongs to the technical field of veterinary medicine preparations. The soluble powder preparation comprises the following components: 30-80% of penicillin V potassium, 1-20% of a beta-lactam inhibitor, 0.5-6% of a pH regulator, 0-10% of an anti-caking agent, 0.02-0.5% of a metal ion chelating agent and the balance of a filler, totaling 100%. The soluble powder is used for drinking water of poultry, and the content of penicillin V potassium in an aqueous solution of the soluble powder is still kept at 99% or above after 12 hours and is still kept at 95% or above after 24 hours. The water solution of the soluble powder does not block a water drinking line in clinical use, so that the clinical use effect and convenience are greatly improved.

The invention relates to the technical field of production of penicillin V potassium, and particularly discloses a preparation method of penicillin V potassium, which comprises the following steps: S1, under the catalysis of penicillin V acylase, enabling 6-APA and methyl phenoxyacetate to react, performing full-water-phase synthesis to obtain a penicillin V solution, filtering out the penicillin V acylase, adding dilute acid into filtrate, and crystallizing to obtain a penicillin V acid crystal; s2, dissolving the penicillin V acid crystal by using an organic solvent I to obtain a solution I, dissolving potassium isooctanoate by using an organic solvent II to obtain a solution II, mixing the solution I and the solution II, reacting and crystallizing to obtain a penicillin V potassium crystal, and filtering, washing and drying to obtain a penicillin V potassium finished product. According to the method, an enzyme method is used for replacing traditional fermentation and extraction methods to produce the penicillin V potassium, so that the process steps and energy consumption can be reduced, and the product quality is effectively improved.

The application discloses a preparation method of near-infrared light enhanced response Cu, Fe-Lyz / AgNPs nanoscale enzyme. The method uses Cu, Fe doped carbon dots as a reducing agent to prepare silver nanoparticles, and then the silver nanoparticles are modified by lysozyme to prepare Cu, Fe-Lyz / AgNPs nanoscale enzyme with positive charges on the surface. The Cu, Fe-Lyz / AgNPs nanoscale enzyme has dual activities of peroxidase-like and glutathione oxidase-like and photo-thermal performance. Meanwhile, the near-infrared light can significantly enhance the POD-like activity of the nanoscale enzyme. The Cu, Fe-Lyz / AgNPs nanoscale enzyme has good antibacterial effect on escherichia coli and staphylococcus aureus. Meanwhile, based on the enhancement of the penicillin G potassium on the Cu, Fe-Lyz / AgNPs oxidase-like activity, the application establishes a rapid colorimetric detection method for the penicillin G potassium, and when the method is used for detecting the penicillin G potassium in blood plasma and milk powder samples, the detection limit is as low as 0.05 mu g / mL, and the method has high reliability and accuracy.

This invention discloses a penicillin V potassium capsule and its preparation method. The preparation method includes: preparing modified polylactic acid glycolic acid copolymer microspheres; mixing penicillin V potassium micropowder with the modified polylactic acid glycolic acid copolymer microspheres, adding fumed silica and anhydrous calcium hydrogen phosphate for further mixing to obtain a blend; granulating the blend using a dry granulation method; filling the obtained granules into capsules, polishing, and packaging; and modifying the polylactic acid glycolic acid copolymer with povidone and ethyl cellulose to obtain the modified polylactic acid glycolic acid copolymer microspheres. This invention designs polylactic acid glycolic acid copolymer microspheres that are double-modified with povidone and ethyl cellulose, resulting in better drug loading flowability, more stable mixing uniformity, and significantly improved flowability compared to penicillin V potassium powder. The addition of fumed silica and anhydrous calcium phosphate further enhances antistatic properties and reduces the powder return rate.

The invention relates to the technical field of production of penicillin V potassium, and particularly discloses a novel process for preparing penicillin V potassium, which comprises the following steps: a) carrying out flocculation treatment on penicillin fermentation liquor, and passing through drum filtering equipment; b) passing the drum filtrate through a roll-type ultrafiltration membrane device, and acidifying and crystallizing the roll-type ultrafiltration membrane to obtain penicillin V acid crystals I; c) recrystallizing the penicillin V acid crystal I to obtain a penicillin V acid crystal II; d) reacting and crystallizing the penicillin V acid crystal II and potassium isooctanoate to obtain penicillin V potassium; e) drying and storing; the molar yield and the quality index of the penicillin V potassium obtained by the novel process are greatly improved compared with those of the existing level.

This invention belongs to the field of biopharmaceutical technology, and particularly relates to a one-pot method for synthesizing amoxicillin. This invention provides a novel enzymatic synthesis route for amoxicillin, specifically including the following steps: (1) Pretreatment of immobilized penicillin G acylase – soaking PGA washed with deionized water in phosphate buffer; (2) dissolving potassium penicillin G and L-p-hydroxyphenylglycine methyl ester in a phosphate buffer and hexane system to obtain an initial reaction solution; (3) mixing the immobilized penicillin G acylase with the initial reaction solution and reacting to obtain an amoxicillin suspension; (4) filtering out the immobilized penicillin G acylase and washing with deionized water, adding hydrochloric acid until the amoxicillin suspension becomes clear, filtration, pH adjustment, cooling crystallization, washing and drying to obtain crude amoxicillin. The entire preparation process is simple, easy to operate, environmentally friendly, has good substrate conversion effect, and a high amoxicillin yield.

The application discloses a marine source beta-lactam antibiotic biodegradation bacterium and application thereof. The marine source beta-lactam antibiotic biodegradation bacterium is isolated and identified from coastal seawater and has the ability of degrading beta-lactam antibiotics. Research shows that the DT01 strain has certain degradation effect on beta-lactam antibiotics, such as penicillin potassium, amoxicillin and ceftriaxone sodium, wherein the DT01 has excellent degradation capacity on penicillin potassium; and the DT01 strain has good temperature and pH adaptability and penicillin potassium resistance, can grow rapidly in a harsh environment and can be better used for the treatment of antibiotic environmental pollution, thereby providing a new effective repair scheme for beta-lactam antibiotic pollution and making contribution to the biological treatment of antibiotic pollution.

The invention provides a preparation method of a high-performance SERS (Surface Enhanced Raman Scattering) substrate based on colloidal crystal template etching and application of the high-performance SERS substrate in detection of benzylpenicillin potassium in medical wastewater. The specific preparation process comprises the following steps: S1, obtaining large-area polystyrene colloid nanospheres which are uniformly, tightly and hexagonally arranged on the water surface by using an inclined plate-assisted gas-liquid interface self-assembly method, and transferring a crystal mask plate to the surface of a clean silicon substrate at a constant speed by using a Czochralski method; s2, putting the silicon wafer integrated with the large-area nanospheres into reactive ion etching (Oxford 100), uniformly etching the nanospheres by adopting oxygen plasma, shortening the distance between the nanospheres, and enhancing local electromagnetic coupling; s3, silver of 60 nm is deposited on the surfaces of the nanospheres in a thermal evaporation mode, and due to the electromagnetic resonance effect of the metal nanoparticles, Raman signals can be further enhanced. And S4, putting the structured SERS substrate and the untreated substrate into a penicillin potassium solution with the concentration of 0.1 mol / L, soaking for 20 minutes, naturally airing, and measuring a Raman signal by using a micro Raman spectrometer.

The invention discloses a preparation method of a near-infrared light enhanced response Cu, Fe-Lyz / AgNPs nano-enzyme, according to the method, Cu and Fe doped carbon dots are used as a reducing agent to prepare silver nanoparticles, then the Cu, Fe-Lyz / AgNPs nano-enzyme with positive charges on the surface is prepared through modification of lysozyme, and the Cu, Fe-Lyz / AgNPs nano-enzyme has dual activities similar to peroxidase and glutathione oxidase and photo-thermal performance, and can be used for preparing the near-infrared light enhanced response Cu, Fe-Lyz / AgNPs nano-enzyme. Meanwhile, the POD-like activity of the nano-enzyme can be obviously enhanced by the near-infrared light; cu and Fe-Lyz / AgNPs have a relatively good antibacterial effect on escherichia coli and staphylococcus aureus; meanwhile, based on the enhancement effect of the penicillin G potassium on the activity of Cu and Fe-Lyz / AgNPs oxidase, the invention establishes a rapid colorimetric detection method for the penicillin G potassium, when the method is used for detecting the penicillin G potassium in plasma and milk powder samples, the detection limit is as low as 0.05 mu g / mL, and the method has relatively high reliability and accuracy.

A mutant penicillin acylase and uses thereof are provided. Compared to the amino acid sequence set forth in SEQ ID NO: 1, the mutant includes at least one mutation selected from the group consisting of: F146αK, F24βR, F71βY, N241βK, G385βY, and G385βR. By introducing mutations into the penicillin acylase derived from Kluyvera citrophila, the invention obtains a mutant enzyme exhibiting enhanced and well-coordinated hydrolytic and synthetic activities. It can be used for the synthesis of β-lactam antibiotics, particularly for the one-step preparation of amoxicillin from penicillin potassium salt, thereby avoiding the isolation of the intermediate 6-APA. The present invention provides a key enzyme for the efficient production of β-lactam antibiotics and is poised to significantly advance the innovation of their manufacturing technology.

The invention belongs to the technical field of pharmaceutical compound synthesis, and relates to a preparation method of penicillin V potassium. The preparation method of the penicillin V potassium comprises synthesis of phenoxyacetyl chloride, preparation of penicillin V acid and preparation of the penicillin V potassium. The preparation method of the penicillin V potassium provided by the invention adopts a chemical synthesis method to prepare the penicillin V potassium, has the advantages of simple process, few production raw materials, few impurities carried by the raw materials, low power consumption, few used equipment and low production cost, and is a method capable of effectively reducing the cost and improving the quality of a finished product.

The invention discloses a preparation method of a high-performance SERS (Surface Enhanced Raman Scattering) substrate based on PDMS (Polydimethylsiloxane) micro-wrinkles and application of the high-performance SERS substrate in penicillin potassium detection, which is characterized by comprising the following steps: firstly, preparing and manufacturing a PDMS sheet with specific hardness and shape, then fixing a bent and stretched PDMS substrate in a special mold, and spraying 60nm gold nanoparticles on the bent surface of the PDMS substrate in a vacuum sputtering manner. After the bent PDMS substrate is unfolded, tiny wrinkles capable of being coupled with hot spots and retaining to-be-detected substances are naturally formed on the surface of the PDMS substrate, the Raman signal intensity can be greatly improved in cooperation with detection of a drip evaporation method, and the method has the advantages of being simple and efficient.

The invention discloses a preparation method of DL-penicillin amine, which comprises the following steps: reacting penicillin G potassium with hydrazine hydrate for ring opening, and then reacting with formaldehyde for cracking, racemization and hydrolysis to obtain the DL-penicillin amine; according to the synthesis scheme, the penicillin G potassium is directly used as a starting raw material, the DL-penicillin amine is synthesized by adopting a one-pot boiling scheme through four-step reaction, the raw materials are low in cost and easy to obtain, the operation is simple, and the method is suitable for industrial production.

The application provides a method for preparing penicillin V potassium by a spray drying method, and belongs to the technical field of drug synthesis. In the method, potassium hydroxide and a mixed solution of strong alkali and weak acid potassium salt are salted at a specific temperature, and then negative pressure spray drying is performed at a low temperature. The method has the advantages of simple process flow, no need to use a large amount of solvent, no solid waste and waste liquid, and environmental protection. In addition, high-purity penicillin V potassium can be obtained. The results of examples show that, according to the method, the penicillin V potassium obtained has the purity of C 16 H 18 N2O5S is not less than 87%, the maximum single impurity is not more than 0.8%, the total impurity is not more than 2.1%, and the polymer is not more than 0.1%.

The application discloses a preparation method of penicillin V potassium, which mainly aims at improving the crystal habit of penicillin V potassium and reducing the content of a key impurity, 4-hydroxy penicillin V potassium. The application also relates to a penicillin V heavy crystal and a method for preparing a potassium salt from penicillin V, which comprises the following operation steps: dissolving raw materials, reacting with alkali to form a salt, solvus crystallization, suction filtration and drying. The method is simple and easy to operate and can be used for preparing penicillin V potassium by using a low-toxicity solvent, and the content of the key impurity, 4-hydroxy penicillin V potassium, can be reduced by more than 50%. The process is low in cost, low in energy consumption, high in efficiency and suitable for industrial production.

A one-step method for synthesizing amoxicillin from penicillin or a salt thereof through an enzyme catalysis is provided. Conventional amoxicillin production requires sequential use of distinct penicillin acylases for hydrolysis and synthesis steps, necessitating isolation of the intermediate 6-aminopenicillanic acid (6-APA) and resulting in elevated manufacturing costs and suboptimal process efficiency. To address these limitations, the inventive method utilizes a mutant of penicillin acylase derived from Kluyvera citrophila as the exclusive biocatalyst in an aqueous reaction system. By reacting penicillin G potassium salt with D-p-hydroxyphenylglycine methyl ester, the process achieves direct amoxicillin synthesis in a single enzymatic step, attaining a product yield of 99%. This method exhibits advantages including fast catalytic rate, high product yield, environmentally friendly pure aqueous reaction system, low cost, and excellent economic benefits.

The invention provides a Delftia sp. PG-8, application thereof and a method for degrading penicillin G potassium. The preservation number of the Delftia sp. Is CCTCC (China Center For Type Culture Collection) NO: M 2024394, and the Delftia sp. Is named as Delftia sp. In taxonomy; the Delftia sp. PG-8 disclosed by the invention has high-concentration resistance to penicillin, can grow by taking penicillin G potassium as a unique carbon source, and can completely degrade the penicillin G potassium under the conditions that the pH is 5-9 and the temperature is 25-40 DEG C; the strain is helpful for efficient degradation of penicillin antibiotics in the environment, and can also provide degradation gene resources for penicillin pollutants in the bioengineering treatment environment. The method has a very strong application prospect in treatment of penicillin pollutants such as restoration of environment polluted by beta-lactamase antibiotics, green biodegradation of mushroom dregs generated in the process of fermentation production of the beta-lactamase antibiotics and the like.

The invention discloses veterinary penicillin v potassium soluble powder and a preparation method thereof, and belongs to the field of veterinary drug preparations. The veterinary penicillin V potassium soluble powder comprises the following components in percentage by weight: 30-90% of penicillin V potassium, 1-20% of a polysaccharide-bioactive peptide compound, 0.5-6% of a pH regulator, 0.02-0.5% of a metal ion chelating agent, 0-10% of an anti-caking agent and the balance of a filling agent. The penicillin V potassium, the polysaccharide-bioactive peptide compound, the metal ion chelating agent and the pH regulator are compounded for use, so that the clinical application value is remarkably improved. The aqueous solution keeps stable components within 24 hours at 25 DEG C, so that frequent replacement of liquid medicine is avoided, and the culture operation cost is reduced; the metal ion chelating agent effectively complexes calcium and magnesium ions in a water source, prevents combination failure of penicillin V potassium and metal ions, inhibits beta-lactamase activity and reduces generation of drug-resistant strains; the synergistic effect of the filler and the anti-caking agent ensures the high water solubility of the powder, avoids the blockage of a drinking line, and improves the group administration efficiency.

The invention discloses a process for synthesizing D-penicillamine, wherein penicillin G potassium salt is used as a raw material, and a monosubstituted hydrazine is subjected to condensation ring opening under the action of a catalyst, and after the reaction is completed, an acetic acid solution is added for acidification, and then n-heptane is added for crystallization to obtain D-penicillamine. The method of the invention is simple to operate, has a short cycle, avoids high-temperature heating reaction conditions, improves raw material conversion rate and reaction yield, realizes a "one-pot method" to obtain a high-purity product with stable quality, and is suitable for industrial production.

The invention belongs to the technical field of medicine preparation processes, and provides a penicillin V potassium tablet and a preparation method thereof. The preparation method comprises the following steps: (1) putting a plain tablet of a penicillin V potassium tablet into a coating pan, preheating, spraying an enteric coating solution onto the rotating plain tablet, drying, and cooling to room temperature to obtain an enteric coated tablet; acrylic resin II and Eudragit RS100 are adopted as a coating agent of the enteric coating solution; (2) putting the enteric coated tablet into a coating pan, preheating, spraying the gastric-soluble coating liquid onto the rotating enteric coated tablet, drying, and cooling to room temperature to obtain a double-layer coated tablet; and a coating agent of the gastric-soluble coating solution adopts acrylic resin IV. The penicillin V potassium tablet prepared by the preparation method disclosed by the invention has a double-layer film coating layer and an extremely good taste masking effect, and can be slowly and stably released in intestinal tracts, and meanwhile, a coating agent adopted by the double-layer film coating layer is close to and single in component.