Culture medium for tissue culture and quick reproduction of Ficus pumia 'Variegata' and reproduction method

A tissue culture and proliferation medium technology, applied in the field of plant tissue culture, can solve the problems of unreported tissue culture technology, unable to meet market demand, low bud induction rate, etc., to accelerate the promotion of improved varieties, and to achieve high plant regeneration efficiency. , improve the effect of planting yield

Inactive Publication Date: 2014-09-10
ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The conventional propagation method of S. chinensis is cutting and layering. The propagation amount is limited, and affected by the season, it cannot meet the market demand. The rapid propagation technology of tissue culture can obtain high-quality and neat seedlings, which opens up a new way for rapid propagation of seedlings. an efficient way
[0004] There are very few reports on the tissue culture of Ficus chinensis, only Li Fang (2000), Jiang Aofang (2007), etc. have been reported, but these reports all have the following problems: 1) The material for tissue culture is the common As a new excellent ornamental plant introduced from abroad, the relevant tissue culture technology has not been reported; 2) The basic medium is B5 or MS, which leads to the decline of seedling quality and low transplant survival rate, generally only 85%; %; 3) Starting from the induction of clustered buds, the operation is more complicated and the bud induction rate is not high (the bud induction rate of MS medium is 46%, and the bud induction rate of B5 medium is 76%)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of method for tissue culture rapid propagation of Yinye Xueli, the following steps are carried out in turn:

[0034] (1) The preparation of the medium, including the components of the basic medium and the medium of each stage of tissue culture and the weight per liter:

[0035] 1) Basic medium: WPM basic medium is used for the cultivation, proliferation and strong seedlings of sterile seedlings, and 1 / 2 WPM is used for rooting culture.

[0036] 2) The sterile seedling medium is: WPM+white sugar 20g / L+agar 8g / L, pH5.6~5.8

[0037] 3) The medium for proliferation and seedling growth is: WPM + 6-BA 2.0 mg / L + NAA 0.2 mg / L + white sugar 25 g / L + agar 5 g / L, pH 5.6-5.8.

[0038] 4) The rooting medium is: 1 / 2WPM + IBA 0.5mg / L + white sugar 30g / L + agar 5g / L, pH 5.6-5.8;

[0039] (2) Cultivation of Tissue Culture Seedlings of Yinye Xueli

[0040] 1) Selection of explants: Choose the robust and tender stems of Yinye Xueli as the explants for tissue culture, soak them ...

Embodiment 2

[0048] In this example, the preparation of the medium in step (1), including the components of the basic medium and the medium of each stage of tissue culture, and the weight per liter are:

[0049] The sterile seedling medium is: WPM + white sugar 25g / L + agar 5g / L, pH 5.6-5.8;

[0050] The proliferation medium was: WPM+ 6-BA 3.0 mg / L + NAA 0.2 mg / L + white sugar 30 g / L + agar 5 g / L, pH 5.6-5.8.

[0051] The rooting medium is: 1 / 2WPM + IBA 1.0 mg / L + white sugar 30 g / L + agar 5 g / L, pH 5.6 to 5.8;

[0052] The remaining steps and processes are the same as those in Example 1.

Embodiment 3

[0054] In this example, the preparation of the medium in step (1), including the components of the basic medium and the medium of each stage of tissue culture, and the weight per liter are:

[0055]The sterile seedling medium is: WPM + white sugar 30g / L + agar 5g / L, pH 5.6-5.8;

[0056] The proliferation medium was: WPM+ 6-BA 3.0 mg / L + NAA 0.3 mg / L + white sugar 30 g / L + agar 5 g / L, pH 5.6-5.8.

[0057] The rooting medium is: 1 / 2WPM + IBA 1.5 mg / L + white sugar 30 g / L + agar 5 g / L, pH 5.6-5.8;

[0058] The remaining steps and processes are the same as those in Example 1.

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Abstract

The invention discloses a culture medium for tissue culture and quick reproduction of Ficus pumia 'Variegata' and a reproduction method. A basic culture medium adopts a WPM (woody plant medium) suitable for woody plants; high-efficiency regeneration of plants in Ficus pumia 'Variegata' tissue culture is realized through continuous subculture and a proper amount of hormone combination; furthermore, explant germfree treatment, culture of explant germfree seedlings and the like are improved. The culture medium is easy to operate, low in pollution rate and high in plant regeneration success rate.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a culture medium and a propagation method for tissue culture and rapid propagation of Fickle sativa. Background technique [0002] Silver Leaf Fructus Lily ( Ficus pumia 'Variegata') belongs to the genus Moraceae, an evergreen woody vine with a small plant type. It is an excellent foliage plant, and it is also an excellent ground cover plant and hedge material. It has high ornamental value, good quality, and has a high Economic benefits, broad prospects for development and utilization, and extremely promising market prospects. [0003] The conventional propagation method of S. chinensis is cutting and layering. The propagation amount is limited, and affected by the season, it cannot meet the market demand. The rapid propagation technology of tissue culture can obtain high-quality and neat seedlings, which opens up a new way for rapid propagation of seedlings. an e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 邹清成朱开元刘慧春马广莹周江华
Owner ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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