Method for preparing Se-enriched rice protein by tissue culture method

A technology of tissue culture and selenium-enriched rice, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of low enrichment concentration, low nutritional value, limited enrichment concentration, etc., and achieve high enrichment concentration , high enrichment efficiency and high efficiency

Active Publication Date: 2014-09-17
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) The planting method has low enrichment efficiency, long production cycle and low enrichment concentration
[0006] The production cycle of the planting method to produce trace element-rich products needs more than 3 months; when the planting method is enriched, the main method is to apply trace element fertilizer, only a part of the trace elements in the fertilizer is absorbed by the crops, and a large amount of trace mineral elements enter the environment, resulting in a large loss; Moreover, only a small

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] This embodiment includes the following steps:

[0030] (1) Cleaning: Harvest rice in the same year, after shelling, collect fresh brown rice and wash it with tap water;

[0031] (2) Disinfection: Soak the brown rice cleaned in step (1) with ozone solution (concentration: 2mg / L) for 30 minutes, then wash with sterile water for 3 times, and cycle 3 times for soaking in ozone solution and cleaning with sterile water ;

[0032] (3) Embryo tissue culture: put the sterilized brown rice in step (2) into a sterilized fermentation container, add sterile soaking solution equivalent to 3 times the weight of sterilized brown rice and soak (25°C) for 8 hours, and the aseptic The soaking solution contains plant growth regulators (2,4-D 4.0mg / L, KT1.0 mg / L, 6-BA2.0 mg / L), sodium selenite (the concentration of selenium in the soaking solution is 30mg / L L); then cultured in a shallow fermentation plate at a temperature of 25°C for 24 hours;

[0033] (4) Collect the culture: centrifug...

Embodiment 2

[0038] This embodiment includes the following steps:

[0039] (1) Cleaning: Collect the by-products of the rice germ when the rice is harvested and milled in the same year, and rinse with tap water;

[0040] (2) Disinfection: soak and disinfect the rice germ cleaned in step (1) with chlorine dioxide solution (500mg / L concentration) for 30 minutes, and then wash it with sterile water for 3 times. L) Soaking and washing with sterile water cycle twice;

[0041] (3) Embryo tissue culture: put the sterilized rice germ in step (2) into a sterilized fermentation container, add a sterile soaking solution equivalent to 3 times the weight of the sterilized rice germ, and the sterile soaking solution contains plant growth regulators agent (2,4-D 3.0mg / L, kinetin KT1.0 mg / L, NAA0.2 mg / L, sodium selenite (concentration of selenium in soaking solution is 20mg / L); temperature 20℃, Ferment for 72 hours, 500mL Erlenmeyer bottle liquid 150mL, shaker speed 90r / min;

[0042] (4) Collect the cu...

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Abstract

The invention relates to a method for preparing Se-enriched rice protein by a tissue culture method. The method comprises the steps of (1) cleaning fresh coarse rice or rice germ; (2) disinfecting the coarse rice or the rice germ; (3) adding inorganic selenium solution and a plant growth regulator, and carrying out tissue culture on the coarse rice or the rice germ; (4) collecting the cultured coarse rice or rice germ, washing and pulping; (5) extracting protein to obtain the Se-enriched rice protein. The Se-enriched rice protein product can be taken as a nutritional supplement used for people who have selenium deficiency.

Description

technical field [0001] The invention relates to a production method of nutritious food, in particular to a production method of selenium-enriched rice protein. Background technique [0002] Recent surveys and studies (Nutrition and Health Survey of Chinese Residents, 2002) show that a considerable part of the population in my country has insufficient dietary intake of zinc, selenium, germanium, calcium, iron, iodine and other trace elements. As far as selenium is concerned, there are 2 / 3 regions in the world and 72% of counties and cities in China are deficient in selenium. Selenium deficiency is more common in developing children, adolescents, pregnant women and nursing mothers. The development of selenium-rich nutritional supplements is of great significance to improving the health of people in our country. [0003] Rice is the staple food of 70% of people in my country, and using rice as a source of mineral nutrition has the advantages of conforming to people's eating ha...

Claims

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Application Information

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IPC IPC(8): A23J1/12A01H4/00
Inventor 付湘晋林亲录任佳丽吴伟吴越
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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