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A kind of vaccine composition and its preparation method and application

A technology of vaccine composition and antigen, applied in the field of vaccine composition, can solve problems such as increased stress response of pig herds, increased risk of infection, cumbersome operating procedures, etc., so as to avoid adverse reactions, maintain a long immune period, simplify The effect of the immunization program

Active Publication Date: 2018-02-13
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of the existing vaccines are that they need to be immunized in stages, at least two injections are needed to prevent multisystem syndrome in weaned piglets and reproductive disorders in sows, the cost is high, and the operation procedures are cumbersome, which increases the risk of infection and causes pig The stress response of the herd increases, which may lead to problems such as immune failure in some herds
However, an unanticipated problem with combination vaccines is the recent recognition that one vaccine in combination vaccines may have an adverse effect on the other, the so-called "antigen interference effect"
It has been found that when two existing vaccines are simply mixed, one or both may lose their potency (see patent application CN101035559A)

Method used

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  • A kind of vaccine composition and its preparation method and application
  • A kind of vaccine composition and its preparation method and application
  • A kind of vaccine composition and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1, Porcine Circovirus, Porcine Japanese Encephalitis Virus and Porcine Parvovirus Triple Inactivated Vaccine Composition, Preparation and Inspection of Porcine Japanese Encephalitis Virus and Porcine Parvovirus Dual Vaccine

[0039] 1. The source of the virus strain

[0040] The PCV2 virus used in the PCV2-PPV-JEV triple vaccine in the embodiments of the present invention is the PCV2 SH strain, and the JEV strain used in the PCV2-PPV-JEV triple vaccine and the PPV-JEV double vaccine in the embodiments of the present invention is The SD-2011 strain, the PPV strain used in the PCV2-PPV-JEV triple vaccine and the PPV-JEV dual vaccine in the embodiment of the present invention is the HN-2011 strain.

[0041] 2. Preparation and inspection of vaccine semi-finished products

[0042] 2.1 Preparation of poisonous seeds for production

[0043] 2.1.1 Preparation of porcine circovirus type 2 SH strain:

[0044] Properly dilute the virus seed of PCV2 SH strain with virus ...

Embodiment 2

[0113] Example 2, this study is to evaluate the immune efficacy of PCV2-PPV-JEV combination triple vaccine with different antigen content and PPV-JEV dual vaccine and PCV2 single vaccine

[0114] 1. Test material

[0115] According to the method in embodiment 1, prepare PCV2-PPV-JEV triple vaccine (PCV2 antigen content is 10 6.0 TCID 50 / ml, PPV is 10 6.0 TCID 50 / ml, JEV is 10 6.0 TCID 50 / ml), recorded as vaccine L; PCV2-PPV-JEV triple vaccine (PCV2 antigen content is 10 6.5 TCID 50 / ml, PPV antigen content is 10 7.0 TCID 50 / ml, JEV antigen content is 10 7.0 TCID 50 / ml), recorded as vaccine M; PCV2-PPV-JEV triple vaccine (PCV2 antigen content is 10 7.0 TCID 50 / ml, PPV antigen content is 10 8.0 TCID 50 / ml, JEV antigen content is 10 8.0 TCID 50 / ml), recorded as vaccine H; low-dose PCV2-PPV-JEV triple vaccine (PCV2 antigen content is 5×10 5.5 TCID 50 / ml, PPV antigen content is 10 7.0 TCID 50 / ml, JEV antigen content is 10 7.0 TCID 50 / ml), recorded a...

Embodiment 3

[0144] Example 3. This study is to evaluate the reproductive performance and growth performance of piglets after PCV2-PPV-JEV triple combination vaccine and PPV-JEV dual vaccine combined with PCV2 single vaccine immunized sows

[0145] 1. Test material

[0146] According to the method in embodiment 1, prepare PCV2-PPV-JEV triple vaccine (PCV2 antigen content is 10 6.5 TCID 50 / ml, PPV antigen content is 10 7.0 TCID 50 / ml, JEV antigen content is 10 7.0 TCID 50 / ml), recorded as vaccine M; PCV2 inactivated vaccine (PCV2 antigen content is 10 6.5 TCID 50 / ml) is recorded as vaccine 2; PPV-JEV dual vaccine (PPV antigen content is 10 7.0 TCID 50 / ml, JEV antigen content is 10 7.0 TCID 50 / ml) was recorded as vaccine 3.

[0147] 2. Design of animal experiments

[0148] 2.1 Design of challenge experiment: 30 gilts were selected and divided into 3 groups with 10 gilts in each group. Before immunization, blood was collected from sows in each group for detection of viremia...

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Abstract

The invention provides a vaccine composition, which comprises: immune amount of porcine circovirus type 2 antigen, immune amount of porcine encephalitis virus antigen, immune amount of porcine parvovirus antigen and adjuvant. The vaccine can effectively prevent and treat weaned piglet multisystem syndrome and sow reproductive disorder caused by porcine circovirus type 2, porcine encephalitis virus and porcine parvovirus. The reaction is small, the serum antibody titer is high, the immunization period lasts for a long time, less time-consuming, less laborious, less stress response to pigs, simplifies the immunization procedure, and reduces production costs and prevention costs.

Description

technical field [0001] The invention relates to a vaccine composition capable of preventing and treating multisystem syndrome of weaned piglets and reproductive disorder diseases of sows. Background technique [0002] Porcine circovirus disease or porcine circovirus-related disease is an immunosuppressive disease mainly caused by porcine circovirus type 2 (Porcinecircovirus2, PCV2), manifested as post-weaning piglet multisystem failure syndrome, porcine dermatitis and nephropathy syndrome syndrome, porcine respiratory disease syndrome, reproductive failure, piglet myocarditis, hyperplastic and necrotizing pneumonia, and central nervous system diseases. Porcine circovirus mainly infects piglets aged 5-12 weeks. The main clinical manifestations are weight loss, dyspnea, jaundice, interstitial pneumonia, lymphadenopathy, hepatitis and nephritis, etc.; histopathological damage is mainly characterized by apoptosis. Characteristic systemic loss of lymphocytes and predominant infi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/23A61P31/14A61P31/20A61P15/08A61K39/12
Inventor 田克恭张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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