Method for detecting traditional Chinese medicine composition for tonifying qi and tonifying kidney

A technology of invigorating qi and invigorating kidney and detection method, applied in the field of medicine, can solve the problems of cumbersome operation, falsification of added ingredients, single detection and so on

Active Publication Date: 2014-09-24
RONGCHANG PHARM ZIBO CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] There are many medicinal flavors in the prescription of this variety. The current quality inspection items seem to be comprehensive, but each ingredient needs to be tested individually, which is cumbersom

Method used

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  • Method for detecting traditional Chinese medicine composition for tonifying qi and tonifying kidney
  • Method for detecting traditional Chinese medicine composition for tonifying qi and tonifying kidney
  • Method for detecting traditional Chinese medicine composition for tonifying qi and tonifying kidney

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Experimental program
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Effect test

Embodiment 1

[0126] (1) Chromatographic conditions and system suitability test: using octadecylsilane bonded silica gel as filler; acetonitrile-methanol with a volume ratio of 95:5 as mobile phase A, and 0.8% acetic acid solution as mobile phase B, Carry out gradient elution according to Table 1; the detection wavelength is 210nm; the flow rate is 1ml / min; the column temperature is 35°C; the number of theoretical plates should not be less than 5000 based on the hesperidin peak;

[0127] (2) Preparation of reference solution: take hesperidin and icariin reference substances respectively, accurately weigh them, add 70% methanol to prepare a mixed solution containing 30 μg of hesperidin and 20 μg of icariin per 1 ml, instant;

[0128] (3) preparation of need testing solution: get oral liquid sample 10ml and put in 100ml volumetric flask, add 70% methanol and dilute to scale, shake up, filter, get the filtrate, to obtain final product;

[0129] (4) Determination method: Precisely draw 20 μl e...

Embodiment 2

[0131] (1) Chromatographic conditions and system suitability test: use octadecylsilane bonded silica gel as filler; use acetonitrile as mobile phase A, use 0.5% acetic acid solution as mobile phase B, carry out gradient elution according to Table 1; detect The wavelength is 270nm; the flow rate is 0.6ml / min; the column temperature is 30°C; the number of theoretical plates should not be less than 5000 based on the hesperidin peak;

[0132] (2) Preparation of reference solution: take hesperidin and icariin reference substances respectively, accurately weigh them, add 70% methanol to prepare a mixed solution containing 30 μg of hesperidin and 20 μg of icariin per 1 ml, instant;

[0133] (3) preparation of need testing solution: get oral liquid sample 10ml and put in 100ml volumetric flask, add 70% methanol and dilute to scale, shake up, filter, get the filtrate, to obtain final product;

[0134] (4) Determination method: Precisely draw 20 μl each of the reference substance solut...

Embodiment 3

[0136] (1) Chromatographic conditions and system suitability test: use octadecylsilane bonded silica gel as filler; use methanol as mobile phase A, use 0.3% acetic acid solution as mobile phase B, carry out gradient elution according to Table 1; detect The wavelength is 350nm; the flow rate is 0.5ml / min; the column temperature is 25°C; the number of theoretical plates calculated based on the hesperidin peak should not be less than 5000;

[0137] (2) Preparation of reference solution: take hesperidin and icariin reference substances respectively, accurately weigh them, add 70% methanol to prepare a mixed solution containing 30 μg of hesperidin and 20 μg of icariin per 1 ml, instant;

[0138] (3) preparation of need testing solution: get oral liquid sample 10ml and put in 100ml volumetric flask, add 70% methanol and dilute to scale, shake up, filter, get the filtrate, to obtain final product;

[0139] (4) Determination method: Precisely draw 20 μl each of the reference substanc...

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Abstract

The invention belongs to the technical field of medicines, and in particular relates to a method for detecting a traditional Chinese medicine composition for tonifying qi and tonifying kidney. The content of hesperidin and icariin is simultaneously measured under one same chromatography condition, and referring to hesperidin, qualitative analysis is performed on relevant components. According to the method, characteristic qualification and content measurement on multiple components are performed simultaneously, the two chemical components, namely, hesperidin and icariin, which are relatively high in content and relatively good in chromatographic separation in a series of sweet dream products, are taken as content measurement parameters for characteristic control on eight characteristic peaks of the components according to retention time, a characteristic qualification and content measurement method of the sweet dream series products is high in completeness, characteristic property and stability, moreover, instruments, chromatographic columns, detection wavelength, chromatography mobile phase, flowing speed, column temperature and the like are optimized, and through inspection on multiple index components, the inherent quality of the sweet dream series products can be comprehensively reflected, and the quality control level of a preparation is improved.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a detection method of a traditional Chinese medicine composition for nourishing qi and tonifying kidney. Background technique [0002] "Sweet Dream Oral Liquid (Capsule)" is a variety included in the Supplement to the 2010 Edition of "Chinese Pharmacopoeia". content determination. [0003] There are many medicinal flavors in the prescription of this variety. The current quality inspection items seem to be comprehensive, but each ingredient needs to be tested individually, which is cumbersome, time-consuming and laborious; a single inspection of a certain ingredient, because it cannot detect the characteristics of the component system, it is easy to falsify the added ingredients. Take advantage of the opportunity. Contents of the invention [0004] The purpose of the present invention is to provide a detection method of a traditional Chinese medicine composition ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 房伟伟翟勇王荔强郭桂秋
Owner RONGCHANG PHARM ZIBO CO LTD
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