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Human immunodeficiency virus (HIV) antibody detection kit and preparation method thereof

A technology for human immunodeficiency and antibody detection, applied in the field of immunodiagnosis, can solve the problems of sensitivity, specificity, and stability to be further improved, and achieve high sensitivity and good specificity

Inactive Publication Date: 2014-10-08
威海威高生物科技有限公司
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AI Technical Summary

Problems solved by technology

[0008] Traditional detection methods for human immunodeficiency virus antibodies include enzyme-linked immunoassay, colloidal gold method, and chemiluminescent detection method. Although these methods have many advantages, they still need to be further improved in terms of detection sensitivity, specificity, and stability.

Method used

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Embodiment Construction

[0018] The kit of the invention adopts microparticle chemiluminescence immunoassay technology to detect whether there is HIV antibody in serum or plasma. The HIV antibody detection kit and its preparation method are specifically described below.

[0019] A human immunodeficiency virus antibody detection kit comprises HIV antibody detection magnetic particle, HIV antibody tracer conjugate, negative control, type I positive control, type II positive control, and analysis buffer. The HIV antibody testing magnetic particles are magnetic particles labeled with HIV antigens (gp41, gp36); the testing HIV antibody tracer conjugates are isoluminol labeled with HIV antigens (gp41, gp36); the type I positive control is containing Human serum / plasma for HIV-1 antibody; type II positive control is rabbit serum / plasma containing HIV-2 antibody; assay buffer is phosphate buffer containing bovine serum albumin.

[0020] The preparation method of the above-mentioned HIV antibody detection kit...

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Abstract

The invention belongs to the technical field of immunologic diagnosis and particularly relates to a human immunodeficiency virus (HIV) antibody detection kit using a microparticle chemiluminiscence method and a preparation method of the kit. The kit is composed of magnetic microparticles for detecting an HIV antibody, a tracing conjugate for detecting the HIV antibody, a negative control, a I type positive control, a II type positive control and an analyzing buffering solution. The invention further discloses the preparation method of the detection kit, which adopts a particle chemiluminiscence immunoassay technology; compared with ELISA (Enzyme-Linked Immunosorbent Assay), the technology has higher sensitivity and specificity and is suitable for the clinical auxiliary diagnosis of HIV.

Description

technical field [0001] The invention relates to an immunodiagnostic technology, in particular to a human immunodeficiency virus antibody detection kit with magnetic particles as a carrier and a preparation method thereof. Background technique [0002] Human Immunodeficiency Virus (HIV) is a lentivirus that infects cells of the human immune system and is a type of retrovirus. It is generally believed that the infection of human immunodeficiency virus leads to AIDS (AIDS, Acquired Immune Deficiency Syndrome, acquired immunodeficiency syndrome, or translated as "AIDS"). A cancerous and fatal disease. AIDS was identified in the United States in 1981 and developed into a global pandemic. By the end of 2003, it had caused more than 20 million deaths. Human Immunodeficiency Virus is also commonly referred to colloquially as "AIDS virus" or "HIV". [0003] The HIV virus is a spherical or oval particle with a diameter of 100-140nm. It is an enveloped RNA retrovirus and belongs to ...

Claims

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Application Information

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IPC IPC(8): G01N33/571G01N33/543G01N21/76
CPCG01N33/571G01N33/54326
Inventor 姚继承李晓燕饶志明闻雯刘海光
Owner 威海威高生物科技有限公司
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