Method for suppressing the effects of ascorbic acid

Abstract

Provided is a method for suppressing the effects of ascorbic acid in the measurement of glycated hemoglobin in a sample containing glycated hemoglobin. A method for measuring glycated hemoglobin in a sample by causing a proteolytic enzyme to act on a sample containing glycated hemoglobin, then reacting the resulting glycated peptide with a glycated peptide oxidase and measuring the resulting hydrogen peroxide; the method for suppressing the effects of ascorbic acid in the measurement of glycated hemoglobin in a sample being characterized in that the reaction of the glycated peptide and glycated peptide oxidase is conducted in the presence of a halogen oxide and a substance selected from the group consisting of substances represented by general formula (I) (in the formula, R1 represents an optionally substituted alkyl) and substances represented by general formula (II) (in the formula, R2 represents an optionally substituted alkyl and --- represents a single bond or double bond).

Description

technical field [0001] The present invention relates to a method for suppressing the influence of ascorbic acid in the measurement of glycated hemoglobin and a reagent for measuring glycated hemoglobin. Background technique [0002] Glycosylated hemoglobin is a glycosylated product in which glucose is bound to hemoglobin. Hemoglobin has a tetramer structure composed of α chain and β chain, and hemoglobin A1c after the N-terminus of β chain is glycated is called hemoglobin A1c, which increases as the blood sugar level increases, so it is used as a diabetes marker in clinical examinations Determination. [0003] As methods for measuring glycosylated hemoglobin, chromatographic methods such as HPLC, electrophoresis, latex immunoagglutination, and other immunoassays using antibodies, enzymes that act on glycated proteins, and enzymes that act on glycated peptides and / or glycated amino acids are known. Enzyme assays, etc. [0004] As an enzymatic measurement method for glycate...

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Problems solved by technology

[0005] However, in the measurement of glycated hemoglobin by this absorbance method, the influence of ascorbic acid present in the sample becomes a problem.

Conventionally, the influence of ascorbic acid has become a problem in the method of measuring the object of measurement in the sample using a hydrogen peroxide measurement system. For the technical problem of suppressing the influence of ascorbic acid, for example, a method using ascorbate oxidase is known (refer to Patent Document 1); the method of using organic complexes such as iron, cobalt, selenium, copper, mercury, nickel (refer to Patent Documents 2 to 4); the method of using iodate (refer to Patent Document 5); using iodate and Using at least one compound selected from phosphoric acid compounds, boric acid compounds, citric acid compounds, malic acid compounds, tartaric acid compounds and oxalic acid compounds as an auxiliary agent for iodate (patent document 6), etc., but inhibits ascorbic acid in glycated hemoglobin The effect of the determination of the effect is not sufficient

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