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A method for disinfecting explants in plant tissue culture

A technology for plant tissue culture and explants, which is applied in the field of explant disinfection in plant tissue culture, and can solve the problems of explant damage, microbial killing, and poor disinfection effect

Active Publication Date: 2016-10-05
INST OF DAFENG MARINE IND NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing disinfection method has poor disinfection effect, and the microorganisms on the material cannot be killed to the maximum extent. Moreover, the explants are easily damaged by the existing disinfection method, which greatly reduces the survival rate of plant tissue culture.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The explant material is cut into small pieces or pieces or flakes or filaments and then sterilized as follows:

[0026] Step 1: Use a brush to remove the sediment and impurities attached to the surface of the explant material under tap water washing;

[0027] Step 2: Soak the explant material pretreated in Step 1 with tap water containing 0.5% antibiotics for 30 minutes, and rinse with tap water for 10 minutes;

[0028] Step 3: Soak the explant material in a mixed solution containing 0.025% mercuric chloride and 0.05% Tween for 10 minutes, wash with sterile water 8 times, and complete the disinfection.

Embodiment 2

[0030] The explant material is cut into small pieces or pieces or flakes or filaments and then sterilized as follows:

[0031] Step 1: Use a brush to remove the sediment and impurities attached to the surface of the explant material under tap water washing;

[0032] Step 2: Soak the explant material pretreated in Step 1 with tap water containing 0.8% antibiotics for 30 minutes, and rinse with tap water for 10 minutes;

[0033] Step 3: Soak the explant material in a mixed solution containing 0.025% mercuric chloride and 0.05% Tween for 10 minutes, wash with sterile water 8 times, and complete the disinfection.

Embodiment 3

[0035] The explant material is cut into small pieces or pieces or flakes or filaments and then sterilized as follows:

[0036] Step 1: Use a brush to remove the sediment and impurities attached to the surface of the explant material under tap water washing;

[0037] Step 2: Soak the explant material pretreated in Step 1 with tap water containing 1% antibiotics for 15 minutes, and rinse with tap water for 5 minutes;

[0038] Step 3: Soak the explant material in a mixed solution containing 0.025% mercuric chloride and 0.15% Tween for 20 minutes, wash it with sterile water for 5 times, and complete the disinfection.

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PUM

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Abstract

The invention discloses a method for disinfecting explants in plant tissue culture. The explant materials are cleaned with tap water, then the cleaned explant materials are soaked with antibiotics, and finally the soaked materials are treated with disinfectants. The method is easy to sterilize, has good bactericidal effect, has low toxicity and is not easy to damage materials.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for disinfecting explants in plant tissue culture. Background technique [0002] Most of the explants used for plant tissue culture are taken from the field, and a large number of microorganisms are attached to the surface, which is a major obstacle for tissue culture. Therefore, the material must be disinfected before inoculation and cultivation. On the one hand, disinfection requires the killing of various microorganisms on the surface of the material, and at the same time, it cannot damage or only slightly damage the tissue material without affecting its growth. Therefore, the disinfection of explants is an important part of plant tissue culture work. [0003] The existing disinfection method has poor disinfection effect, and the microorganisms on the material cannot be killed to the maximum extent. Moreover, the explants are easily damaged by the existi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 胡燕花张本厚陈集双陈斌
Owner INST OF DAFENG MARINE IND NANJING UNIV OF TECH