The preparation method of chondroitin sulfate

A technology of chondroitin sulfate and cartilage, applied in the biological field, can solve the problems that organic impurities cannot be completely removed, affect the clarity and color of the product solution, and achieve stable product quality, simple production and operation, and high efficiency.

Active Publication Date: 2016-05-18
江苏麦德森制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In the production process of chondroitin sulfate, enzymatic hydrolysis, resin adsorption, alcohol precipitation and oxidative decolorization are mainly used to purify chondroitin sulfate. During this process, some organic impurities brought by raw and auxiliary materials cannot be completely removed, which affects the clarity of the product solution. and color

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: produce high-quality chondroitin sulfate according to the following steps

[0024] (1) Poaching: Submerge 100 kg of cartilage raw materials in water, raise the temperature to 80°C, and cook for 24 hours to peel off the muscle tissue attached to the surface of the cartilage, and remove the cartilage;

[0025] (2) Cooking: Put the boiled cartilage obtained in step (1) into a kettle, steam at 100°C for more than 4 hours, and collect the upper layer of oil;

[0026] (3) Enzymolysis: Submerge the softened cartilage obtained in step (2) in 100 liters of water, add 3 kg of sodium chloride, adjust the pH to 8 with alkali, raise the temperature to 55°C, and then add 0.4 kg of 2079 alkaline protease to keep pH8, keep warm at 55°C and stir for 6 hours, heat up to boiling for 12 minutes, filter;

[0027] (4) Resin adsorption: 80 liters of 201 anion resin is adsorbed, eluted, and the eluate is collected;

[0028] (5) Alcohol precipitation: adjust the pH of the eluent...

Embodiment 2

[0033] Embodiment 2: produce high-quality chondroitin sulfate according to the following steps

[0034] (1) Poaching: Submerge 100 kg of cartilage raw materials in water, raise the temperature to 90°C, and cook for 18 hours to peel off the muscle tissue attached to the surface of the cartilage, and remove the cartilage;

[0035] (2) Cooking: Put the boiled cartilage obtained in step (1) into a kettle, steam at 100°C for more than 4 hours, and collect the upper layer of oil;

[0036] (3) Enzymatic hydrolysis: Submerge the softened cartilage obtained in step (2) in 100 liters of water, add 3 kg of sodium chloride, adjust the pH to 8.5 with alkali, raise the temperature to 57.5°C, add 0.4 kg of 2079 alkaline protease, and keep the pH at 8 .5, keep warm at 57.5°C and stir for 5 hours, heat up to boiling for 15 minutes, and filter;

[0037] (4) Resin adsorption: 80 liters of 201 anion resin is adsorbed, eluted, and the eluate is collected;

[0038] (5) Alcohol precipitation: adju...

Embodiment 3

[0043] Embodiment 3: produce high-quality chondroitin sulfate according to the following steps

[0044] (1) Poaching: Submerge 100 kg of cartilage raw materials in water, raise the temperature to 100°C, and cook for 12 hours to peel off the muscle tissue attached to the surface of the cartilage, and remove the cartilage;

[0045] (2) Cooking: Put the boiled cartilage obtained in step (1) into a kettle, steam at 100°C for more than 4 hours, and collect the upper layer of oil;

[0046] (3) Enzymatic hydrolysis: Submerge the softened cartilage obtained in step (2) in 100 liters of water, add 3 kg of sodium chloride, adjust the pH to 9 with alkali, raise the temperature to 60°C, add 400 grams of 2079 alkaline protease, and keep the pH at 9 , keep warm at 60°C and stir for 4 hours, heat up to boiling for 18 minutes, and filter;

[0047] (4) Resin adsorption: 80 liters of 201 anion resin is adsorbed, eluted, and the eluate is collected;

[0048] (5) Alcohol precipitation: adjust t...

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PUM

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Abstract

The invention discloses a preparation method of chondroitin sulfate, which comprises the following steps: (1) boiling with water; (2) steaming; (3) enzymolysis; (4) resin adsorption; (5) alcohol precipitation; (6) decolorization; (7) impurity removal; (8) alcohol precipitation; and (9) dehydration and drying. The method is applied to acidic sodium nitrate conditions, the impurities in the chondroitin sulfate react to generate high-solubility substances and then are removed by alcohol precipitation, the clarity and color of the product solution satisfy the injection requirements, the mild conditions are controllable, the production process is simple to operate and suitable for large-scale production, the product quality is stable, and the chondroitin sulfate recovery rate is up to 80% above.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of chondroitin sulfate. Background technique [0002] Chondroitin sulfate, English name chondroitinsulfate, is a kind of polymeric acidic mucopolysaccharide based on the cartilage of animals (pigs, cattle, horses, sheep, chickens, sharks, etc.), and its molecular structure is β-D-glucuronic acid and N-acetylgalactosamine is a polysaccharide chain composed of repeating disaccharide units formed by β1→3 glycosidic bonds. It is a white or slightly yellow powder with strong water absorption, easily soluble in water to form a viscous liquid, insoluble in organic solvents such as alcohol, acetone, ether and chloroform. Its salts are relatively stable to heat. A large number of studies have shown that chondroitin sulfate has blood lipid-lowering, anti-atherosclerosis and anti-viral hepatitis activities, and has been used in the prevention and treatment of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
Inventor 刘榜惠
Owner 江苏麦德森制药有限公司
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