Method for producing low-molecular chondroitin sulfate by catalytic process

A chondroitin sulfate and production method technology, applied in the biological field, can solve the problems of complex operation, severe conditions, many impurities, etc., and achieve the effects of high efficiency, simple operation and stable product quality

Active Publication Date: 2014-12-03
江苏麦德森制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the production process of low-molecular chondroitin sulfate, acid hydrolysis, enzymatic hydrolysis or oxidation are mainly used to break the glycosidic bond of chondroitin sulfate to prepare low-molecular chondroitin sulfate. However, the reaction conditions of acid hydrolysis of chondroitin sulfate are severe and difficult to control, and the cost of enzymatic hydrolysis High is not suitable for large-scale production, but the conditions of ordinary catalytic oxidation method are severe, there are many residual metal ion catalysts in the solution after the reaction, and there are too many impurities in the solution, which need ion exchange to remove completely, affecting the separation and purification of products, and the operation is complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Production of low molecular chondroitin sulfate according to the following steps

[0024] (1) Boiled: 100 kilograms of cartilage materials are immersed in water, heated to 80°C, and boiled for 24 hours to peel off the muscle tissue attached to the cartilage surface and remove the cartilage;

[0025] (2) Steaming: Put the boiled cartilage obtained in step (1) into a kettle, steam at 100°C for more than 4 hours, and collect the upper layer of oil;

[0026] (3) Enzymatic hydrolysis: submerge the softened cartilage obtained in step (2) with 100 liters of water, add 3 kg of sodium chloride, adjust the pH to 8 with alkali, raise the temperature to 55°C, add 1 kg of alkaline protease, and keep the pH at 8. Keep at 55°C and stir for 6 hours, heat up to boiling for 12 minutes, and filter;

[0027] (4) Alcohol precipitation: adjust the pH of the filtrate obtained in step (3) to 6, then add alcohol to make the solution alcohol concentration reach 60%, and settle for more than 1...

Embodiment 2

[0032] Example 2: Production of low molecular chondroitin sulfate according to the following steps

[0033] (1) Boiled: 100 kg of cartilage raw materials are immersed in water, heated to 90°C, and boiled for 18 hours to peel off the muscle tissue attached to the cartilage surface and remove the cartilage;

[0034] (2) Steaming: Put the boiled cartilage obtained in step (1) into a kettle, steam at 100°C for more than 4 hours, and collect the upper layer of oil;

[0035] (3) Enzymatic hydrolysis: submerge the softened cartilage obtained in step (2) with 100 liters of water, add 3 kg of sodium chloride, adjust the pH to 8.5 with alkali, raise the temperature to 57.5°C, and then add 1.5 kg of 2709 alkaline protease to maintain pH 8. .5, keep 57.5℃ and stir for 5 hours, heat to boiling for 15 minutes, and filter;

[0036] (4) Alcohol precipitation: adjust the pH of the filtrate obtained in step (3) to 6.5, and then add alcohol to make the solution alcohol concentration reach 60%, and preci...

Embodiment 3

[0041] Example 3: Production of low molecular chondroitin sulfate according to the following steps

[0042] (1) Boiled: 100 kilograms of cartilage raw materials are immersed in water, heated to 100°C, and boiled for 12 hours to peel off the muscle tissue attached to the cartilage surface and remove the cartilage;

[0043] (2) Steaming: Put the boiled cartilage obtained in step (1) into a kettle, steam at 100°C for more than 4 hours, and collect the upper layer of oil;

[0044] (3) Enzymatic hydrolysis: submerge the softened cartilage obtained in step (2) with 100 liters of water, add 3 kg of sodium chloride, adjust the pH to 9 with alkali, raise the temperature to 60°C, and then add 2 kg of 2709 alkaline protease to keep the pH 9 , Keep at 60℃ and stir for 4 hours, heat up to boiling for 18 minutes, and filter;

[0045] (4) Alcohol precipitation: adjust the pH of the filtrate obtained in step (3) to 7, and then add alcohol to make the solution alcohol concentration reach 60%, and prec...

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PUM

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Abstract

The invention discloses a method for producing low-molecular chondroitin sulfate by a catalytic process, which comprises the following steps: (1) boiling with water; (2) steaming; (3) enzymolysis; (4) alcohol precipitation; (5) catalysis; (6) ultrafiltration; (7) alcohol precipitation; and (8) dehydration and drying. By using the catalyst Cu / ZSM-5 molecular sieve to degrade the chondroitin sulfate, the method has the advantages of mild and controllable conditions, fewer residual metal ion impurities in the degraded solution and no need of ion exchange resin removal, is simple to operate in the production process, and is suitable for large-scale production; and the product quality is stable, and the chondroitin sulfate recovery rate is up to higher than 80%.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for producing low-molecular chondroitin sulfate by catalytic method. Background technique [0002] Chondroitin sulfate, English name chondroitin sulfate, is a kind of polymeric acidic mucopolysaccharide made from the cartilage of animals (pigs, cattle, horses, sheep, chickens, sharks, etc.), and its molecular structure is β-D-glucuronic acid It is a polysaccharide chain composed of repeating disaccharide units formed by β1→3 glycosidic bonds with N-acetylgalactosamine. It is a white or slightly yellow powder with strong water absorption, easily soluble in water to form a viscous liquid, insoluble in organic solvents such as alcohol, acetone, ether and chloroform. Its salts are relatively stable to heat. The biological activity of chondroitin sulfate varies with molecular weight. Its pharmacological activity is closely related to its molecular weight, and the size of its m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 刘榜惠
Owner 江苏麦德森制药有限公司
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