Method for producing low-molecular chondroitin sulfate by catalytic process
A chondroitin sulfate and production method technology, applied in the biological field, can solve the problems of complex operation, severe conditions, many impurities, etc., and achieve the effects of high efficiency, simple operation and stable product quality
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Embodiment 1
[0023] Example 1: Production of low molecular chondroitin sulfate according to the following steps
[0024] (1) Boiled: 100 kilograms of cartilage materials are immersed in water, heated to 80°C, and boiled for 24 hours to peel off the muscle tissue attached to the cartilage surface and remove the cartilage;
[0025] (2) Steaming: Put the boiled cartilage obtained in step (1) into a kettle, steam at 100°C for more than 4 hours, and collect the upper layer of oil;
[0026] (3) Enzymatic hydrolysis: submerge the softened cartilage obtained in step (2) with 100 liters of water, add 3 kg of sodium chloride, adjust the pH to 8 with alkali, raise the temperature to 55°C, add 1 kg of alkaline protease, and keep the pH at 8. Keep at 55°C and stir for 6 hours, heat up to boiling for 12 minutes, and filter;
[0027] (4) Alcohol precipitation: adjust the pH of the filtrate obtained in step (3) to 6, then add alcohol to make the solution alcohol concentration reach 60%, and settle for more than 1...
Embodiment 2
[0032] Example 2: Production of low molecular chondroitin sulfate according to the following steps
[0033] (1) Boiled: 100 kg of cartilage raw materials are immersed in water, heated to 90°C, and boiled for 18 hours to peel off the muscle tissue attached to the cartilage surface and remove the cartilage;
[0034] (2) Steaming: Put the boiled cartilage obtained in step (1) into a kettle, steam at 100°C for more than 4 hours, and collect the upper layer of oil;
[0035] (3) Enzymatic hydrolysis: submerge the softened cartilage obtained in step (2) with 100 liters of water, add 3 kg of sodium chloride, adjust the pH to 8.5 with alkali, raise the temperature to 57.5°C, and then add 1.5 kg of 2709 alkaline protease to maintain pH 8. .5, keep 57.5℃ and stir for 5 hours, heat to boiling for 15 minutes, and filter;
[0036] (4) Alcohol precipitation: adjust the pH of the filtrate obtained in step (3) to 6.5, and then add alcohol to make the solution alcohol concentration reach 60%, and preci...
Embodiment 3
[0041] Example 3: Production of low molecular chondroitin sulfate according to the following steps
[0042] (1) Boiled: 100 kilograms of cartilage raw materials are immersed in water, heated to 100°C, and boiled for 12 hours to peel off the muscle tissue attached to the cartilage surface and remove the cartilage;
[0043] (2) Steaming: Put the boiled cartilage obtained in step (1) into a kettle, steam at 100°C for more than 4 hours, and collect the upper layer of oil;
[0044] (3) Enzymatic hydrolysis: submerge the softened cartilage obtained in step (2) with 100 liters of water, add 3 kg of sodium chloride, adjust the pH to 9 with alkali, raise the temperature to 60°C, and then add 2 kg of 2709 alkaline protease to keep the pH 9 , Keep at 60℃ and stir for 4 hours, heat up to boiling for 18 minutes, and filter;
[0045] (4) Alcohol precipitation: adjust the pH of the filtrate obtained in step (3) to 7, and then add alcohol to make the solution alcohol concentration reach 60%, and prec...
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