Method for producing low molecular weight chondroitin sulfate by catalytic method
A technology of chondroitin sulfate and its production method, which is applied in the biological field, can solve problems such as complex operation, severe conditions, and many impurities, and achieve the effects of high efficiency, simple operation, and stable product quality
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Embodiment 1
[0023] Embodiment 1: produce low molecular weight chondroitin sulfate according to the following steps
[0024] (1) Poaching: Submerge 100 kg of cartilage raw materials in water, raise the temperature to 80°C, and cook for 24 hours to peel off the muscle tissue attached to the surface of the cartilage, and remove the cartilage;
[0025] (2) Cooking: Put the boiled cartilage obtained in step (1) into a kettle, steam at 100°C for more than 4 hours, and collect the upper layer of oil;
[0026] (3) Enzymolysis: Submerge the softened cartilage obtained in step (2) with 100 liters of water, add 3 kg of sodium chloride, adjust the pH to 8 with alkali, raise the temperature to 55°C, and then add 1 kg of alkaline protease to keep the pH at 8. Stir at 55°C for 6 hours, heat up to boiling for 12 minutes, and filter;
[0027] (4) Alcohol precipitation: adjust the pH of the filtrate obtained in step (3) to 6, then add alcohol to make the alcohol concentration of the solution reach 60%, an...
Embodiment 2
[0032] Embodiment 2: produce low molecular weight chondroitin sulfate according to the following steps
[0033] (1) Poaching: Submerge 100 kg of cartilage raw materials in water, raise the temperature to 90°C, and cook for 18 hours to peel off the muscle tissue attached to the surface of the cartilage, and remove the cartilage;
[0034] (2) Cooking: Put the boiled cartilage obtained in step (1) into a kettle, steam at 100°C for more than 4 hours, and collect the upper layer of oil;
[0035] (3) Enzymolysis: Submerge the softened cartilage obtained in step (2) with 100 liters of water, add 3 kg of sodium chloride, adjust the pH to 8.5 with alkali, raise the temperature to 57.5°C, and then add 1.5 kg of 2709 alkaline protease to keep the pH at 8 .5, keep warm at 57.5°C and stir for 5 hours, heat up to boiling for 15 minutes, and filter;
[0036] (4) Alcohol precipitation: Adjust the pH of the filtrate obtained in step (3) to 6.5, then add alcohol to make the alcohol concentrati...
Embodiment 3
[0041] Embodiment 3: produce low molecular weight chondroitin sulfate according to the following steps
[0042] (1) Poaching: Submerge 100 kg of cartilage raw materials in water, raise the temperature to 100°C, and cook for 12 hours to peel off the muscle tissue attached to the surface of the cartilage, and remove the cartilage;
[0043] (2) Cooking: Put the boiled cartilage obtained in step (1) into a kettle, steam at 100°C for more than 4 hours, and collect the upper layer of oil;
[0044] (3) Enzymolysis: Submerge the softened cartilage obtained in step (2) with 100 liters of water, add 3 kg of sodium chloride, adjust the pH to 9 with alkali, raise the temperature to 60°C, and then add 2 kg of 2709 alkaline protease to keep the pH at 9 , keep warm at 60°C and stir for 4 hours, heat up to boiling for 18 minutes, and filter;
[0045] (4) Alcohol precipitation: Adjust the pH of the filtrate obtained in step (3) to 7, then add alcohol to make the alcohol concentration of the s...
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