Preparation method of chondroitin sulfate
A chondroitin sulfate and cartilage technology, applied in the biological field, can solve the problems that organic impurities cannot be completely removed, affect the clarity and color of the product solution, and achieve the effects of stable product quality, simple operation and easy removal after production.
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Embodiment 1
[0023] Embodiment 1: produce high-quality chondroitin sulfate according to the following steps
[0024] (1) Poaching: Submerge 100 kg of cartilage raw materials in water, raise the temperature to 80°C, and cook for 24 hours to peel off the muscle tissue attached to the surface of the cartilage, and remove the cartilage;
[0025] (2) Cooking: Put the boiled cartilage obtained in step (1) into a kettle, steam at 100°C for more than 4 hours, and collect the upper layer of oil;
[0026] (3) Enzymolysis: Submerge the softened cartilage obtained in step (2) in 100 liters of water, add 3 kg of sodium chloride, adjust the pH to 8 with alkali, raise the temperature to 55°C, and then add 0.4 kg of 2079 alkaline protease to keep PH8, keep warm at 55°C and stir for 6 hours, heat up to boiling for 12 minutes, filter;
[0027] (4) Resin adsorption: 80 liters of 201 anion resin is adsorbed, eluted, and the eluate is collected;
[0028] (5) Alcohol precipitation: Adjust the pH of the eluent...
Embodiment 2
[0033] Embodiment 2: produce high-quality chondroitin sulfate according to the following steps
[0034] (1) Poaching: Submerge 100 kg of cartilage raw materials in water, raise the temperature to 90°C, and cook for 18 hours to peel off the muscle tissue attached to the surface of the cartilage, and remove the cartilage;
[0035] (2) Cooking: Put the boiled cartilage obtained in step (1) into a kettle, steam at 100°C for more than 4 hours, and collect the upper layer of oil;
[0036] (3) Enzymatic hydrolysis: Submerge the softened cartilage obtained in step (2) in 100 liters of water, add 3 kg of sodium chloride, adjust the pH to 8.5 with alkali, raise the temperature to 57.5°C, add 0.4 kg of 2079 alkaline protease, and keep the pH at 8 .5, keep warm at 57.5°C and stir for 5 hours, heat up to boiling for 15 minutes, and filter;
[0037] (4) Resin adsorption: 80 liters of 201 anion resin is adsorbed, eluted, and the eluate is collected;
[0038] (5) Alcohol precipitation: Adju...
Embodiment 3
[0043] Embodiment 3: produce high-quality chondroitin sulfate according to the following steps
[0044] (1) Poaching: Submerge 100 kg of cartilage raw materials in water, raise the temperature to 100°C, and cook for 12 hours to peel off the muscle tissue attached to the surface of the cartilage, and remove the cartilage;
[0045] (2) Cooking: Put the boiled cartilage obtained in step (1) into a kettle, steam at 100°C for more than 4 hours, and collect the upper layer of oil;
[0046] (3) Enzymatic hydrolysis: Submerge the softened cartilage obtained in step (2) in 100 liters of water, add 3 kg of sodium chloride, adjust the pH to 9 with alkali, raise the temperature to 60°C, and then add 400 grams of 2079 alkaline protease to keep the pH at 9 , keep warm at 60°C and stir for 4 hours, heat up to boiling for 18 minutes, and filter;
[0047] (4) Resin adsorption: 80 liters of 201 anion resin is adsorbed, eluted, and the eluate is collected;
[0048] (5) Alcohol precipitation: A...
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