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A kind of colloidal gold rapid detection test strip of amisulfaphos and preparation method thereof

A technology for detecting test paper and colloidal gold, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of ELISA method application limitations, difficulty in satisfying fast, online detection, and detection results are not intuitive enough, and achieve the result display image, which is easy to promote , the effect of judging the image

Inactive Publication Date: 2016-09-07
JURONG AGRI TECH PROMOTION CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Instrumental analysis methods include high performance liquid chromatography (High performance liquid chromatography, HPLC), gas chromatography (Gas chromatography, GC), thin layer chromatography (Thin layer chromatography, TLC), liquid-mass tandem method (HPLC-MS) and gas chromatography. - mass tandem method (GC-MS), etc. Although these instrumental analysis methods can achieve high detection sensitivity, they require complex and expensive instruments and professional operators. , the need for online testing
ELISA (Enzyme Linked Immunosorbent Assay) method is a commonly used immunoassay method, but it has relatively many operating steps, a long detection time, the detection result is not intuitive enough, and it cannot be detected online.
Therefore, the application of ELISA method is greatly limited.

Method used

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  • A kind of colloidal gold rapid detection test strip of amisulfaphos and preparation method thereof
  • A kind of colloidal gold rapid detection test strip of amisulfaphos and preparation method thereof
  • A kind of colloidal gold rapid detection test strip of amisulfaphos and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A colloidal gold rapid detection test strip of amisulfaphos, said test strip contains liner 1 and sample pad 2, gold standard binding pad 3, cellulose membrane 4 and water-absorbing pad 7 arranged in sequence on the liner, so The liner 1 is a tough material that does not absorb water, and the tough material is made of a hard plastic strip. The gold-labeled binding pad 3 is adsorbed with a sulfaphos gold-labeled antibody, and the cellulose membrane 4 has an invisible The detection line 5 and the invisible control line 6, the invisible detection line 5 is printed with a carrier protein solution coupled with the sulfafos hapten, and the invisible control line 6 is printed with a rabbit anti-mouse IgG antibody. The water-absorbing pad 7 is water-absorbing filter paper or oil filter paper with strong water-absorbing capacity. Amiphos gold-labeled antibody is a colloidal gold-labeled amiphos monoclonal antibody. The carrier protein coupled with the sulfasulfon hapten is bovi...

Embodiment 2

[0037] It is basically the same as Example 1, except that the tough material is made of non-absorbent hard paper strips, and the water-absorbing pad 7 is oil filter paper with strong water-absorbing capacity. The carrier protein coupled with the sulfasulfon hapten is chicken ovalbumin OVA. The sample pad 2 is made of nylon film, polyvinylidene fluoride film and polyester film. Cellulose membranes are pure cellulose membranes.

Embodiment 3

[0039] It is basically the same as in Example 1, except that the tough material is made of hard plastic strips, and the water-absorbing pad 7 is water-absorbing filter paper with strong water-absorbing capacity. The carrier protein coupled with the sulfasulfon hapten is human serum albumin HAS. The sample pad 2 is made of polyvinylidene fluoride film and polyester film. The cellulose film is a carboxylated cellulose film.

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Abstract

The invention discloses a famphur colloidal gold rapid test strip. The strip comprises a liner as well as a sample pad, a gold conjugate pad, a cellulose film and a water absorption pad which are sequentially arranged on the liner. A famphur colloidal gold antibody is adsorbed in the gold conjugate pad. The cellulose film is provided with an invisible test line printed with a carrier protein solution coupled to a famphur hapten and an invisible control line printed with a rabbit anti-mouse IgG antibody. The famphur colloidal gold rapid test strip has the advantages of high specificity, high sensitivity, simpleness, rapidness as well as the visualized, intuitive and accurate result showing.

Description

technical field [0001] The invention belongs to the technical field of immune analysis of pesticide residues, and in particular relates to a rapid detection test strip of amisulfafos colloidal gold and a preparation method thereof. Background technique [0002] Amiphos (Famphur) is a broad-spectrum organophosphorus insecticide used to control pests in stored grains and on various foliage crops, and is also used to control mosquito adults, flies, aquatic larvae and hygienic pests. Its mechanism of action is to inhibit the activity of acetylcholinesterase. Acetylcholine is a neurotransmitter, and excess acetylcholine overstimulates acetylcholine receptors, leading to the death of target organisms. However, some creatures that were not the intended target were also poisoned, including humans, fish and other creatures. [0003] At present, the detection methods for pesticide residues are mainly instrumental analysis methods and enzyme-linked immunoassay methods (ELISA). Inst...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/531
CPCG01N33/025G01N33/532G01N33/558
Inventor 韩志成
Owner JURONG AGRI TECH PROMOTION CENT
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