Bioactive bacterial cellulose-zein composite film and preparation method thereof

A technology of zein and bacterial cellulose film, which is applied in biological packaging, pharmaceutical formulations, flexible coverings, etc., can solve the problems of long time-consuming and low practical utility of bacterial cellulose composite film materials, and achieves the goal of material preparation. fast and efficient results

Active Publication Date: 2014-12-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the deficiencies of the above-mentioned prior art, the object of the present invention is to provide a method for preparing a high-strength bioactive bacterial cellulo

Method used

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  • Bioactive bacterial cellulose-zein composite film and preparation method thereof
  • Bioactive bacterial cellulose-zein composite film and preparation method thereof
  • Bioactive bacterial cellulose-zein composite film and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Step 1, preparation of bacterial cellulose membrane

[0042] Medium composition: 2.0% D-glucose, 0.5% peptone, 0.5% yeast extract, 0.115% citric acid, 0.27% Na 2 HPO 4 12H 2 O, the rest is distilled water, pH5.0

[0043] Cultivation method: inoculate Acetobacter xylinum into the seed culture solution, and vibrate at 30°C for 12 hours, then inoculate 10% of the fermentation medium, shake well, and culture at 30°C for 5 days to obtain a bacterial cellulose film.

[0044] Step 2, purification of bacterial cellulose membrane

[0045] Take the bacterial cellulose membrane prepared above, wash it with clean water for 3 times, soak it in a NaOH solution with a concentration of 0.2mol / L, boil it at 90°C for 30 minutes, take it out, then soak the membrane in distilled water, and boil it at 90°C 30min, change the water, repeat 3 times, then soak it in distilled water until neutral to obtain the purified bacterial cellulose membrane;

[0046] Step 3, preparation of bacterial ce...

Embodiment 2

[0075] Step 1, preparation of bacterial cellulose membrane

[0076] Medium composition: 1.8% D-glucose, 0.45% peptone, 0.45% yeast extract, 0.120% citric acid, 0.25% Na 2 HPO 4 12H 2 O, the rest is distilled water, pH4.8.

[0077] Cultivation method: inoculate Acetobacter xylinum into the seed culture solution, shake and cultivate at 30°C for 12 hours, then inoculate the fermentation medium at a ratio of 15%, shake well, and culture at 30°C for 5 days to obtain bacterial cellulose membrane.

[0078] Step 2, purification of bacterial cellulose membrane

[0079] Take the bacterial cellulose membrane prepared above, wash it with clean water for 3 times, soak it in 0.4mol / L NaOH solution, boil it at 90°C for 30min, take it out, then soak the membrane in distilled water, boil it at 90°C for 30min, replace water, repeated 4 times, and then soaked in distilled water until neutral to obtain the purified bacterial cellulose membrane;

[0080] Step 3, preparation of bacterial cellu...

Embodiment 3

[0112] Step 1, preparation of bacterial cellulose membrane

[0113] Medium composition: 2.2% D-glucose, 0.6% peptone, 0.6% yeast extract, 0.110% citric acid, 0.30% Na 2 HPO 4 12H 2 O, the rest is distilled water, pH 4.0.

[0114] Cultivation method: inoculate Acetobacter xylinum into the seed culture solution, shake and cultivate at 30°C for 12 hours, then inoculate the fermentation medium at a ratio of 12%, shake well, and culture at 30°C for 5 days to obtain bacterial cellulose membrane.

[0115] Step 2, purification of bacterial cellulose membrane

[0116] Take the bacterial cellulose membrane prepared above, wash it with clean water for 3 times, soak it in 0.2mol / L KOH solution, boil it at 90°C for 30min, take it out, then soak the membrane in distilled water, boil it at 90°C for 30min, replace water, repeated 5 times, and then soaked in distilled water until neutral to obtain the purified bacterial cellulose membrane;

[0117] Step 3, preparation of bacterial cellulo...

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Abstract

The invention discloses a bioactive bacterial cellulose-zein composite film and a preparation method thereof. The method comprises the following steps: preparing and purifying a bacterial cellulose film; uniformly stirring the purified bacterial cellulose in distilled water, shearing by using a high-speed shearing machine, and homogenizing by high pressure microfluidization to obtain a nano-fiber dispersion liquid; dissolving zein and water-insoluble bioactive substances in an ethanol-water solution to obtain an alcoholic solution; dissolving sodium caseinate in water, stirring and adding the solution into the alcoholic solution to obtain a mixed solution; removing the ethanol, and centrifuging to obtain bioactive molecule enriched zein nanoparticles; mixing the nano-fiber dispersion liquid and the bioactive molecule enriched zein nanopartiles, uniformly stirring, and carrying out ultrasonic treatment; and sucking and filtering in vacuum, and quickly forming a film; and drying. The composite film material has the advantages of excellent mechanical performance, biocompatibility and biodegradability, and has managed bioactivities of bacteria resistance, oxidation resistance and the like.

Description

technical field [0001] The invention relates to a method for preparing a high-strength bioactive bacterial cellulose-zein composite film material. The raw materials are all food-grade and fully degradable biological materials, which belong to biomedical materials such as wound dressings, and food industry technology. field. Background technique [0002] Bacterial Cellulose (BC) is produced by the fermentation of bacteria such as Acetobacter xylinum. It is a nanofibrous polymer without branch chains composed of β-1,4 glycosidic bonds, which is attracting more and more attention. . Compared with traditional cellulose, bacterial cellulose has many advantages, such as high purity, high mechanical strength, high water holding capacity, good biocompatibility, breathable moisture permeability, biodegradability, non-toxicity, and Strong adsorption capacity and other characteristics, so it is widely used in biomedical materials, food, paper, cosmetics (biological fiber mask, eye ma...

Claims

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Application Information

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IPC IPC(8): A61L15/44A61L15/32A61L15/28A61L15/42A61K8/73A61K8/64A61K8/02C08L1/02C08L89/00C08J5/18B65D65/38
CPCY02W90/10
Inventor 杨晓泉王丽英万芝力
Owner SOUTH CHINA UNIV OF TECH
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