Supercharge Your Innovation With Domain-Expert AI Agents!

Lateral flow immunochromatographic assay product and preparation method for detecting Listeria monocytogenes

A technology of Listeria monocytogenes and immunochromatography, applied in the field of lateral flow immunochromatographic assay products and preparation for the detection of Listeria monocytogenes, can solve the problems of slow detection speed and limited on-site use, and achieve rapid detection , highly sensitive assay, strong specificity

Inactive Publication Date: 2016-09-14
SHENZHEN INT TRAVEL HEALTHCARE CENT
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of them require high technology and conditions and relatively slow detection speed, which limits the on-site use in port quarantine.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lateral flow immunochromatographic assay product and preparation method for detecting Listeria monocytogenes
  • Lateral flow immunochromatographic assay product and preparation method for detecting Listeria monocytogenes
  • Lateral flow immunochromatographic assay product and preparation method for detecting Listeria monocytogenes

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0048] According to an embodiment of the present invention, the preparation method of the lateral flow immunochromatographic assay product comprises the following steps:

[0049] (1) Preparation of carboxyl-modified magnetic nanoparticle-labeled probes

[0050] Using suitable carboxyl-modified magnetic nanoparticles, after activating the carboxyl groups on its surface, chemical coupling is used to directionally link the Listeria monocytogenes antibody to the surface of the carboxyl-modified magnetic nanoparticles.

[0051] (2) Coating of antigen / antibody at the T line and C line in the test area

[0052] Using a film-spraying instrument, spray Listeria monocytogenes coating antibody on the T line of the test area, and spray anti-mouse IgG antibody on the C line.

[0053] (3) Coating of the labeled probe at the sample pad

[0054] Spray the anti-Listeria monocytogenes antibody labeled with magnetic microspheres (magnetic nanoparticle-labeled Listeria monocytogenes antibody) o...

Embodiment 1

[0062] Example 1, Preparation of Lateral Flow Immunochromatography Assay Paper for Detecting Listeria Monocytogenes

[0063] (1) Preparation of magnetic nanoparticles-labeled Listeria monocytogenes antibody

[0064] Magnetic Fe modified with polycetyl maleate (PMAH) with a saturation magnetization of 40 emu / g, an average diameter of 100 nm, and a diameter variation coefficient (CV) of 15% 3 o 4 Water-soluble nanocrystals (purchased from Shenzhen Thales Technology Co., Ltd., catalog number MP-2) are used as carboxyl-modified magnetic nanoparticles, with an affinity constant of 10 for Listeria monocytogenes. 8 m -1 The anti-Listeria monocytogenes mouse monoclonal antibody (purchased from Abnnova Company, No. MAB0532) was used as the Listeria monocytogenes antibody to be labeled, and the magnetic nanoparticle-labeled Listeria monocytogenes antibody was prepared according to the following method:

[0065] Take 2.5 mg of the above-mentioned carboxy-modified magnetic nanoparticle...

Embodiment 2

[0071] Example 2, Sensitivity detection of lateral flow immunochromatographic assay test paper for detecting Listeria monocytogenes

[0072] The sensitivity of the lateral flow immunochromatographic assay test paper for detecting Listeria monocytogenes in Example 1 was determined by using formaldehyde-inactivated Listeria monocytogenes as the sample to be tested.

[0073] The standard strain of Listeria monocytogenes (NICPBP54002) was taken out from the refrigerator at -20°C, activated with LEB medium, inactivated with 0.3% formaldehyde for 12 hours, and prepared into a series of concentrations (10 9 、10 8 、10 7 、10 6 、10 5 、10 4 、10 3 、10 2 , 10CFU / mL), respectively added in the lateral flow immunochromatographic assay test paper for detecting Listeria monocytogenes obtained in Example 1, and using magnetic resonance detector MAR (MagnaBioSciences, 8094-101-01&8094- 101-02) Read RMU (relative magnetic field strength). Detection steps: return the sample to be tested to...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides a lateral flow immunoassay determination product for detecting listeria monocytogenes and a preparation method. The product comprises a sample cushion, a water absorption cushion and a coating film which are connected with one another, wherein the coating film is provided with a detection line and a quality control line which are separated from each other; the coating film is arranged between the sample cushion and the water absorption cushion; the sample cushion is loaded with a magnetic nano particle marked listeria monocytogenes antibody; the detection line is coated by a listeria monocytogenes coating antibody; the quality control line is coated by a second antibody which is specifically combined with the magnetic nano particle marked listeria monocytogenes antibody; and the magnetic nano particle marked listeria monocytogenes antibody is a polymer formed by a listeria monocytogenes antibody to be marked and carboxyl-modified magnetic nano particles through a peptide bond in a covalent binding manner. The determination product is used for detecting the listeria monocytogenes, has high sensitivity and strong specificity and is rapid and simple, and can realize objective determination.

Description

technical field [0001] The invention relates to a lateral flow immunochromatographic assay product and a preparation method thereof, in particular to a lateral flow immunochromatographic assay product and a preparation method for detecting Listeria monocytogenes (Listeria monocytogenes). Background technique [0002] Lateral flow immunoassay (LFIA, Lateralflow immunoassay) is a new type of immunoassay that appeared at the end of the 20th century. It is simple and fast, and is widely used in the detection of various viruses such as HIV, hepatitis B virus and hormones. By combining immunolabeling technology and membrane chromatography technology, the results can be judged in a very short time without special conditions, and it has become an important and convenient immunoassay method. [0003] Listeria monocytogenes (Listeria monocytogenes), also known as Listeria monocytogenes. Listeria is a type of bacteria that commonly exists in the environment, and Listeria monocytogenes...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/569G01N33/531
CPCG01N33/531G01N33/56911G01N33/577
Inventor 史蕾向军俭马岚赵芳吴峰闻一鸣冬冰
Owner SHENZHEN INT TRAVEL HEALTHCARE CENT
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com