The preparation method of patulin
A technology of patulin and ethyl acetate, applied in the field of microorganisms, can solve the problems of restricting research work, being expensive, increasing the cost of scientific research work and the like
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0040] 1. Fermentation
[0041] Take 28 250mL triangular flasks, fill each bottle with 100g peeled apple pieces as the culture medium, add 8 layers of gauze plugs, and sterilize at 121°C for 20 minutes. Inoculate the slant of the PDA test tube with Penicillium extensa FP2 strain, activate it at 28°C for 7 days, add sterile water to the slant of each test tube to prepare a concentration of 10 7 cfu / mL spore suspension, insert 1mL concentration of 10 into each Erlenmeyer flask filled with apple culture medium 7 The cfu / mL spore suspension was cultured at 28°C for 10 days in the dark to obtain a culture.
[0042] 2. Separation
[0043] Take 28 bottles of culture, grind them into paste respectively, add 100mL of ethyl acetate to each bottle, shake and extract at 150-200r / min in the dark for 1 hour, let stand for 5-10 minutes to separate layers, and collect the ethyl acetate extract in the upper layer , add ethyl acetate 100mL / 100g to the lower culture, shake and extract at 150-...
Embodiment 2
[0059] In the purification step 3 of this example, the crude extract of patulin was freeze-dried at -70°C and 100 Pa to dryness, fully dissolved with 100 mL of ethyl acetate, and the ethyl acetate was rotatably evaporated at 40°C to 10-15 mL. Purified by 300 mesh silica gel column chromatography, eluted with an eluent with a volume ratio of acetone and dichloromethane of 1:20, packed the column by wet method, loaded the sample by dry method, and detected the eluent in each tube by thin layer chromatography In case of containing patulin; combine the eluents containing patulin, rotatively evaporate to dryness at 40°C in the dark, and dissolve in 10-15mL of a solvent with a volume ratio of petroleum ether to ethyl acetate of 1:1 ; 200~300 mesh silica gel column chromatography again, be 1:1 eluent elution with sherwood oil and ethyl acetate volume ratio, wet method packing column, dry method load sample, detect each tube with thin layer chromatography The case of patulin in the el...
Embodiment 3
[0062] In the purification step 3 of this example, the crude extract of patulin was freeze-dried at -70°C and 100 Pa to dryness, fully dissolved with 100 mL of ethyl acetate, and the ethyl acetate was rotatably evaporated at 40°C to 10-15 mL. Purified by 300 mesh silica gel column chromatography, eluted with an eluent with a volume ratio of acetone and dichloromethane of 1:40, packed the column by wet method, loaded the sample by dry method, and detected the eluent in each tube by thin layer chromatography In case of containing patulin; combine the eluents containing patulin, rotatively evaporate to dryness at 40°C in the dark, and dissolve in 10-15mL of a solvent with a volume ratio of petroleum ether to ethyl acetate of 1:1 ; 200~300 mesh silica gel column chromatography again, be 1:1 eluent elution with sherwood oil and ethyl acetate volume ratio, wet method packing column, dry method load sample, detect each tube with thin layer chromatography The case of patulin in the el...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More