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Detection and analysis method of shellfish diarrhea toxin based on cell image sensor

An image sensor and sensor technology, applied in image analysis, image data processing, individual particle analysis, etc., can solve the problems of high price, high detection cost, and huge equipment, and achieve the effect of low cost, simple operation steps, and low cost

Inactive Publication Date: 2016-08-17
ZHEJIANG UNIV
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  • Claims
  • Application Information

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Problems solved by technology

Although HPLC-MS has the advantages of a wide range of detection, its equipment is bulky and expensive
ELISA and other enzyme-linked immunoassay techniques also have disadvantages such as high price and high detection cost

Method used

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  • Detection and analysis method of shellfish diarrhea toxin based on cell image sensor
  • Detection and analysis method of shellfish diarrhea toxin based on cell image sensor
  • Detection and analysis method of shellfish diarrhea toxin based on cell image sensor

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Embodiment Construction

[0033] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but the present invention is not limited.

[0034] The method for detecting and analyzing shellfish diarrheal toxins based on a cell image sensor of the present invention is implemented on a shellfish diarrheal toxin detection and analysis system, such as figure 1As shown, the shellfish diarrhea toxin detection and analysis system includes: CCD camera 1, cardiomyocyte sensor, inverted microscope 3 and computer 5; wherein, cell image sensor 2 is fixed on the stage of inverted microscope 3; CCD camera 1 is fixed on Above the inverted microscope 3, the image signal of the cell image sensor culture plate is collected; the CCD camera 1 is connected with the computer 5 through the USB connection line 4, so that the collected signal is transmitted to the computer 5 for post-processing; the method includes the following steps:

[0035] (1) Cultivation ...

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Abstract

The invention discloses a shellfish diarrhea toxin detection and analysis method based on a cell image sensor. The method constructs a high-performance and low-cost cell image sensor, adopts image acquisition, RGB image conversion to grayscale image, image binarization, image The matrix and peak detection algorithm calculate and detect the change of the beating image during the mechanical beating of cardiomyocytes, and realize the detection of the rate, amplitude and beating interval of the mechanical beating of cardiomyocytes; The response of the sample working solution is used to construct a standard map; the response of the cell image sensor to unknown concentrations of shellfish diarrhea toxins is compared with the standard map to calculate the toxin concentration semi-quantitatively; compared with the existing shellfish diarrhea The toxin detection and analysis method of the present invention has the advantages of low cost, simple and convenient operation, and can perform long-term and intuitive evaluation of toxin toxicity.

Description

technical field [0001] The invention relates to a shellfish diarrhea toxin detection and analysis technology, in particular to a shellfish diarrhea toxin detection and analysis method based on a cell image sensor. Background technique [0002] Diarrheal toxins in marine aquatic products are a kind of highly toxic non-protein toxins, which are formed by the accumulation of harmful organic substances in the body of shellfish by preying on planktonic algae. People can cause poisoning and even death after eating. At present, the detection methods of diarrheal toxins in marine aquatic products in China usually use mouse biological assay (MBA), high performance liquid chromatography-mass spectrometry (HPLC-MS) and enzyme-linked immunoassay technology (ELISA). The amount of experiments required by the MBA method is huge and there are great individual differences, and the virulence level of the toxin may be underestimated. Although HPLC-MS has the advantages of a wide detection ra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/10G06T7/00
Inventor 王平苏凯麒邹玲王琴胡宁黎洪波邹瞿超曹端喜
Owner ZHEJIANG UNIV