A method for extracting flavonoid active ingredients in traditional Chinese medicine tangerine peel
A technology of active ingredients and flavonoids, applied in the field of natural medicine extraction and detection, to achieve the effect of improving environmental protection benefits and reducing pollution
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[0048] The specific steps of the preparation method of the tangerine peel reference substance solution are as follows: respectively take an appropriate amount of reference substance of hesperidin, nobietin, and tangeretin, accurately weigh them, mix them in a brown measuring bottle, add methanol to make each 1 mL of hesperidin 500 μg, nobiletin 500 μg, and orange peeletin 500 μg solution, namely the reference substance solution.
[0049] The molecular sieve adsorbent used in the examples of the present invention was purchased from Nanjing Xianfeng Nano Material Technology Co., Ltd., and the model is SBA-15.
Embodiment 1
[0051] Weigh 25 mg each of 4 parts of tangerine peel medicinal powder and add them into 4 groups of mortars respectively, and weigh 25 mg each of different kinds of adsorbents (molecular sieve, Florisil, C18 reversed-phase silica gel, alumina), and add them respectively. Grind with tangerine peel medicinal powder for 150s in 4 groups of mortars. Take four 1mL solid-phase extraction cartridges, add sieves to the bottom, fill the cartridges with ground solids, fill the fillers, and add sieves to the top. Place the filled 4 small columns on the solid phase extraction apparatus. Take 0.5mL of methanol, inject it into the small column, turn on the air pump of the solid phase extraction apparatus, and elute the small column one by one. The bottom was collected with four 1.5mL centrifuge tubes respectively. After the elution, the liquid in each centrifuge tube was vortexed for 10s, then put into the centrifuge, and centrifuged at 13000rpm for 5min. The supernatant was taken, which...
Embodiment 2
[0060] Weigh 5 portions of tangerine peel medicinal powder, each 25 mg, and add them to 5 groups of mortars, and weigh molecular sieves of different masses (6.25 mg, 12.5 mg, 25 mg, 50 mg, 75 mg), add them to 5 groups of mortars to grind with tangerine peel medicinal powder 150s. Take five 1mL solid-phase extraction cartridges, add sieves to the bottom, fill the cartridges with ground solids, fill the fillers, and add sieves to the top. Place the filled 5 small columns on the solid phase extraction apparatus. Take 0.5mL of methanol, inject it into the small column, turn on the air pump of the solid phase extraction apparatus, and elute the small column one by one. The bottom was collected with five 1.5mL centrifuge tubes respectively. After the elution, the liquid in each centrifuge tube was vortexed for 10s, then put into the centrifuge, and centrifuged at 13000rpm for 5min. The supernatant was collected as a sample, and analyzed by ultra-high performance liquid chromatogr...
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