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A method for extracting flavonoid active ingredients in traditional Chinese medicine tangerine peel

A technology of active ingredients and flavonoids, applied in the field of natural medicine extraction and detection, to achieve the effect of improving environmental protection benefits and reducing pollution

Active Publication Date: 2017-12-29
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Up to now, the matrix solid-phase dispersion extraction technology based on the trace system has not been reported in the whole field of analytical chemistry, and molecular sieves are used as the adsorbent, combined with the matrix solid-phase dispersion extraction technology, there is no such thing as extraction and detection of the active ingredients of traditional Chinese medicine. According to reports, this method is a blank for the research field of extraction and detection of traditional Chinese medicine tangerine peel

Method used

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  • A method for extracting flavonoid active ingredients in traditional Chinese medicine tangerine peel
  • A method for extracting flavonoid active ingredients in traditional Chinese medicine tangerine peel
  • A method for extracting flavonoid active ingredients in traditional Chinese medicine tangerine peel

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preparation example Construction

[0048] The specific steps of the preparation method of the tangerine peel reference substance solution are as follows: respectively take an appropriate amount of reference substance of hesperidin, nobietin, and tangeretin, accurately weigh them, mix them in a brown measuring bottle, add methanol to make each 1 mL of hesperidin 500 μg, nobiletin 500 μg, and orange peeletin 500 μg solution, namely the reference substance solution.

[0049] The molecular sieve adsorbent used in the examples of the present invention was purchased from Nanjing Xianfeng Nano Material Technology Co., Ltd., and the model is SBA-15.

Embodiment 1

[0051] Weigh 25 mg each of 4 parts of tangerine peel medicinal powder and add them into 4 groups of mortars respectively, and weigh 25 mg each of different kinds of adsorbents (molecular sieve, Florisil, C18 reversed-phase silica gel, alumina), and add them respectively. Grind with tangerine peel medicinal powder for 150s in 4 groups of mortars. Take four 1mL solid-phase extraction cartridges, add sieves to the bottom, fill the cartridges with ground solids, fill the fillers, and add sieves to the top. Place the filled 4 small columns on the solid phase extraction apparatus. Take 0.5mL of methanol, inject it into the small column, turn on the air pump of the solid phase extraction apparatus, and elute the small column one by one. The bottom was collected with four 1.5mL centrifuge tubes respectively. After the elution, the liquid in each centrifuge tube was vortexed for 10s, then put into the centrifuge, and centrifuged at 13000rpm for 5min. The supernatant was taken, which...

Embodiment 2

[0060] Weigh 5 portions of tangerine peel medicinal powder, each 25 mg, and add them to 5 groups of mortars, and weigh molecular sieves of different masses (6.25 mg, 12.5 mg, 25 mg, 50 mg, 75 mg), add them to 5 groups of mortars to grind with tangerine peel medicinal powder 150s. Take five 1mL solid-phase extraction cartridges, add sieves to the bottom, fill the cartridges with ground solids, fill the fillers, and add sieves to the top. Place the filled 5 small columns on the solid phase extraction apparatus. Take 0.5mL of methanol, inject it into the small column, turn on the air pump of the solid phase extraction apparatus, and elute the small column one by one. The bottom was collected with five 1.5mL centrifuge tubes respectively. After the elution, the liquid in each centrifuge tube was vortexed for 10s, then put into the centrifuge, and centrifuged at 13000rpm for 5min. The supernatant was collected as a sample, and analyzed by ultra-high performance liquid chromatogr...

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Abstract

The invention discloses a method for extracting flavonoid active ingredients in traditional Chinese medicine tangerine peel. The flavonoid active ingredients are one or more of hesperidin, nobietin and orange peelin. The method is as follows: Take tangerine peel medicinal powder and molecular sieve adsorbent, mix them in a mass ratio of 1:0.25~3, put them into a mortar and grind, take a solid phase extraction column, add a sieve plate at the bottom, fill the column with the ground solid, and fill the filler After solidification, add a sieve plate to the top, place the filled column on the solid-phase extraction apparatus, take the eluent, inject it into the column, turn on the air pump of the solid-phase extraction instrument, and elute the column. The centrifuge tube collects the eluate, and after the elution is completed, the eluate in the centrifuge tube is vortexed and centrifuged, and the supernatant is taken as the flavonoid active ingredient extract. The invention adopts the molecular sieve as the adsorbent and combines the solid-phase dispersion extraction technology of the matrix to extract and detect the flavonoid active ingredients in the tangerine peel medical material efficiently and in an environment-friendly manner.

Description

technical field [0001] The invention belongs to the field of extraction and detection of natural medicines. It relates to a micro-extraction method of traditional Chinese medicine. Specifically, it relates to a new method for extracting trace amounts of flavonoid active ingredients in dried tangerine peel of traditional Chinese medicine by using molecular sieve as an adsorbent and combining matrix solid-phase dispersion extraction technology. Background technique [0002] Chenpi is the dry ripe pericarp of Rutaceae plant orange (Citrus reticulata Blanco) and its cultivars. Tangerine peel has the effects of drying dampness and resolving phlegm, regulating qi and invigorating the spleen, and its medicinal materials contain a large number of active ingredients. In addition to volatile oil, tangerine peel mainly contains flavonoids. The flavonoids can be divided into two types: flavonoid glycosides and polymethoxylated flavonoids. Among them, flavonoid glycosides are mainly d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28
Inventor 曹君胡帅帅刘训高曹婉庞潇卿代菡镔达建华
Owner HANGZHOU NORMAL UNIVERSITY
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