A kind of fingerprint identification method of Caoguo and grass cardamom

Abstract

The invention provides a fingerprint chromatogram identification method for rapidly identifying amomum tsaoko and alpinia katsumadai. The method comprises the following steps: preparing a test solution via ultrasound-assisted extraction or cold-maceration extraction by taking ethyl acetate, petroleum ether or dichloromethane as an extracting solvent; and carrying out measurement to obtain a fingerprint chromatogram under a certain condition via a GC-MS method so as to identify amomum tsaoko and alpinia katsumadai. Scientific experiments prove that the method is simple, convenient and rapid to operate, and the measured fingerprint chromatogram is high in repeatability and strong in characteristic property, so that the method can be applied to the rapid identification and the quality control on amomum tsaoko and alpinia katsumadai.

Description

technical field [0001] The invention relates to the field of identification of traditional Chinese medicines, in particular to a method for identification of fingerprints of Tsaoko and cardamom herbaceous medicinal materials. Background technique [0002] Caoguo, also known as Caoguozi, Caoguo Kernel, was first recorded in "Compendium of Materia Medica", and the 2010 edition of "Chinese Pharmacopoeia" records that Caoguo is the dry mature fruit of the ginger plant Amomumtsao-koCrevostet Lemaire. The effect of malaria; the grass cardamom is the dry and nearly mature seed of the ginger plant AlpiniaKatsumadaiHayata, which has the effect of drying dampness and promoting qi, warming the middle and relieving vomiting; grass fruit and grass cardamom have different effects, and their clinical applications are also different, but because they are both Gingeraceae medicinal materials are very similar in appearance and smell, so they are often confused and used. In order to distingui...

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Problems solved by technology

Recently, there have been reports of using steam distillation or supercritical fluid to extract tsaoguo or cardamom volatile oil, and then use GC-MS method to determine the fingerprint of its volatile oil, but because these methods usually require a large amount of medicinal materials, the extraction process is more troublesome , the extraction time is generally relatively long; and the steam distillation method may cause damage and loss of some active ingredients due to high temperature and long extraction time, so it is not suitable as a rapid identification method for medicinal materials

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