A kind of fingerprint identification method of Caoguo and grass cardamom

A technology of fingerprint and cardamom, which is applied in the field of fingerprint identification of grass fruit and cardamom grass, can solve the problems of rapid identification method of inappropriate medicinal materials, destruction of active ingredients, troublesome extraction process, etc., and achieves a simple and fast extraction and analysis process, Easy to operate and avoid the effect of high extraction temperature

Inactive Publication Date: 2016-04-06
GUANGXI UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recently, there have been reports of using steam distillation or supercritical fluid to extract tsaoguo or cardamom volatile oil, and then use GC-MS method to determine the fingerprint of its volatile oil, but because these methods usually require a large amount of medicinal materials, the extraction process is more troublesome , the extraction time is generally relatively long; and the steam distillation method may cause damage and loss of some active ingredients due to high temperature and long extraction time, so it is not suitable as a rapid identification method for medicinal materials

Method used

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  • A kind of fingerprint identification method of Caoguo and grass cardamom
  • A kind of fingerprint identification method of Caoguo and grass cardamom
  • A kind of fingerprint identification method of Caoguo and grass cardamom

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 The Determination of the Common Patterns of Fingerprints of Caoguo and Cardamom Grass Medicinal Materials

[0033] 1 Experimental materials

[0034]1.1 Experimental medicinal materials: Tsaoguo and grass cardamom medicinal materials used in the experiment were purchased from large pharmacies in various provinces and cities across the country. Caoguo medicinal material was identified as the dried and mature fruit of Amomumtsao-koCrevostet Lemaire by Professor Cai Yi of the Chinese Medicine Identification Department of Guangxi University of Traditional Chinese Medicine; fruit.

[0035] 1.2 Pre-treatment of experimental medicinal materials: Take grass fruit and grass cardamom kernels from various places of origin, grind them with a grinder, pass through a 40-mesh sieve, and set aside.

[0036] 1.3 Experimental reagents: ethyl acetate (analytical grade, Sinopharm Chemical Reagent Co., Ltd.), anhydrous sodium sulfate (analytical grade, Tianjin Damao Chemical Reag...

Embodiment 2

[0055] Example 2 Effects of Different Extraction Solvents and Extraction Methods on the GC-MS Fingerprint of Tsaoguo

[0056] 1. Experimental materials: The herbs used in this experiment were purchased from Jiajiakang Pharmacy in Heshan District, Yiyang City, Hunan Province. They were identified by Professor Cai Yi from the Department of Traditional Chinese Medicine Identification, Guangxi University of Traditional Chinese Medicine as dried and mature grass fruits of the genus Amomumtsao-kocrevostetlemaire in the family Zingiberaceae. fruit.

[0057] 1.2 Pretreatment of experimental medicinal materials: same as in Example 1.

[0058] 1.3 Experimental reagents: petroleum ether (60-90°C), dichloromethane, ethyl acetate (analytical grade, Sinopharm Chemical Reagent Co., Ltd.), anhydrous sodium sulfate (analytical grade, Tianjin Damao Chemical Reagent Factory).

[0059] 2. Experimental apparatus: with embodiment 1.

[0060] 3. GC-MS analysis conditions: with embodiment 1.

[00...

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Abstract

The invention provides a fingerprint chromatogram identification method for rapidly identifying amomum tsaoko and alpinia katsumadai. The method comprises the following steps: preparing a test solution via ultrasound-assisted extraction or cold-maceration extraction by taking ethyl acetate, petroleum ether or dichloromethane as an extracting solvent; and carrying out measurement to obtain a fingerprint chromatogram under a certain condition via a GC-MS method so as to identify amomum tsaoko and alpinia katsumadai. Scientific experiments prove that the method is simple, convenient and rapid to operate, and the measured fingerprint chromatogram is high in repeatability and strong in characteristic property, so that the method can be applied to the rapid identification and the quality control on amomum tsaoko and alpinia katsumadai.

Description

technical field [0001] The invention relates to the field of identification of traditional Chinese medicines, in particular to a method for identification of fingerprints of Tsaoko and cardamom herbaceous medicinal materials. Background technique [0002] Caoguo, also known as Caoguozi, Caoguo Kernel, was first recorded in "Compendium of Materia Medica", and the 2010 edition of "Chinese Pharmacopoeia" records that Caoguo is the dry mature fruit of the ginger plant Amomumtsao-koCrevostet Lemaire. The effect of malaria; the grass cardamom is the dry and nearly mature seed of the ginger plant AlpiniaKatsumadaiHayata, which has the effect of drying dampness and promoting qi, warming the middle and relieving vomiting; grass fruit and grass cardamom have different effects, and their clinical applications are also different, but because they are both Gingeraceae medicinal materials are very similar in appearance and smell, so they are often confused and used. In order to distingui...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 覃洁萍何俏明李耀华梁臣艳
Owner GUANGXI UNIV OF CHINESE MEDICINE
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