New method for sand fixing by using blue algae
A new method, cyanobacteria technology, is applied in the field of bio-environmental engineering, which can solve the problems of poor bio-environmental compatibility, inability of plants to survive, and short sand-fixing cycle, and achieve simple operation, sand-fixing effect, environmental protection, and cost-saving effects
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Embodiment 1
[0025] a. Select cyanobacteria with curing effect Nostoc linckia FACHB-103, using conventional liquid JM medium at a temperature of 25°C and a light intensity of 45 micromole / m 2 The enrichment culture was carried out for 7 days under the condition of photon yield per second, and the absorbance value of the final algae liquid at 680 nanometers was 0.2;
[0026] b. Place the algae liquid obtained from the enrichment culture in step a on a low-speed stirrer, stir for 30 minutes at a speed of 500 rpm, and add the cationic metal compound MgCl 2 , formulated as the metal cation Mg 2+ Mixed algae solution with a concentration of 2 mol / L;
[0027] c, adjust the pH of the mixed algae solution obtained by enrichment in step b to be 4, and then spray 20 milliliters of the mixed algae solution on 100 g of dry surface sandy soil surface layer of Fukang Beishawo, Gurbantunggut Desert, Xinjiang Uygur Autonomous Region, Put the sand sprayed with the mixed algae solution in a daily dry env...
Embodiment 2
[0029] a. Select cyanobacteria with curing effect Nostoc linckia FACHB-103, using conventional liquid JM medium at a temperature of 25°C and a light intensity of 45 micromole / m 2 The enrichment culture was carried out for 15 days under the condition of photon yield per second, and the absorbance value of the final algae liquid at 680 nanometers was 1.0;
[0030] b. Place the algae liquid obtained from the enrichment culture in step a on a low-speed stirrer, stir for 60 minutes at a speed of 1000 rpm, and add the cationic metal compound MnCl 2 , formulated as a metal cation Mn 2+ Mixed algae solution with a concentration of 1 mol / L;
[0031] c, adjust the pH of the mixed algae solution obtained by enrichment in step b to be 7, and then spray 30 milliliters of the mixed algae solution evenly on 100 g of the surface sandy soil surface of Fukang Beishawo, Gurbantonggut Desert, Xinjiang Uygur Autonomous Region, After spraying the mixed algae solution, the sandy soil was cultured...
Embodiment 3
[0033] a. Select cyanobacteria with curing effect Nostoc linckia FACHB-103, using conventional liquid JM medium at a temperature of 25°C and a light intensity of 45 micromole / m 2 The enrichment culture was carried out for 20 days under the condition of photon yield per second, and the absorbance value of the final algae liquid at 680 nanometers was 1.5;
[0034] b. Place the algae liquid obtained from the enrichment culture in step a on a low-speed stirrer, stir for 90 minutes at a speed of 1500 rpm, and add the cationic metal compound MgCl 2 , formulated as the metal cation Mg 2 Mixed algae solution with a concentration of 0.1 mol / L;
[0035] c, adjust the pH of the mixed algae solution obtained by enrichment in step b to be 10, and then spray 20 milliliters of the mixed algae solution on 100 g of the surface sandy soil surface of Fukang Beishawo, Gurbantunggut Desert, Xinjiang Uygur Autonomous Region, and After spraying the mixed algae solution, the sandy soil was culture...
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