A kind of method of actinomycetes sand fixation
A technology of actinomycetes and bacterial liquid, applied in the field of biological environmental engineering, can solve the problems of poor biological environment compatibility, inability of plants to survive, short sand fixation cycle, etc., and achieve the effects of simple operation, environmental protection of sand fixation, and wide application.
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[0027] The invention is not limited to the following examples:
[0028] A kind of novel method of actinomycetes sand-fixing of the present invention, carry out according to the following steps:
[0029] a. Source and culture of actinomycetes: select Actinomycesnaeslundii, place the strain in nutrient broth medium for recovery, transfer it to an Erlenmeyer flask containing 50 mL of nutrient broth medium, and adjust the pH to 4-11 , and then place the Erlenmeyer flask under the conditions of 130 rpm and 30°C for 48 hours to obtain the actinomycetes bacterial liquid;
[0030] b. Induction and solidification of actinomycetes acclimatization and enrichment: the actinomycetes obtained in step a are placed in a nutrient soup with a mass content of 1-20% urea and 0.01-0.5 mol / liter calcium ion as nutrient broth and rice washing Mix in water medium, place the mixed solution under the condition of rotating speed of 130 rpm and temperature of 30°C for 5-7 days to enrich and obtain solid...
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