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Preparation and application of plant source acaricide

A plant extract and extract technology, applied in the field of preparation and application of plant toxins, can solve the problems of grassland pollution, drug residues, etc., and achieve the effect of high-efficiency acaricidal activity

Inactive Publication Date: 2015-02-11
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the drugs used to treat animal mites are mainly chemical synthesis and antibiotic drugs, such as ivermectin, abamectin, lindane EC, and mite net. Drug residues and grass pollution have caused problems in public health, food safety and ecological environment

Method used

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  • Preparation and application of plant source acaricide
  • Preparation and application of plant source acaricide
  • Preparation and application of plant source acaricide

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Experimental program
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Effect test

Embodiment Construction

[0015] 1 Materials and methods

[0016] 1.1 Test drugs and materials

[0017] 1.1.1 The tested medicinal materials

[0018] Eupatorium adenophorum, collected in Liangshan Prefecture, Sichuan Province in July 2012, the leaves were dried in the shade and crushed for later use.

[0019] 1.1.2 Tested mites

[0020] Collected from the auricles of naturally infected rabbits. The auricles of sick rabbits are covered with blood scabs, which are moist and lumpy near the inner side of the ear canal, and the outside is relatively dry and roll-like. The affected rabbits are itchy and uneasy. Use small tweezers to peel off the inner skin of the ear canal and toes. Put it in a glass petri dish, apply a small amount of mite repellant—sulfur ointment on the edge of the petri dish, heat it at about 37°C, and use a pick needle to pick out vigorous mites for the test. According to the shape of the mites, it was identified as rabbit itch mite ( Psoroptes cuniculi ), scabies mit...

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Abstract

The invention relates to a high efficiency, low toxicity and environment-friendly plant insecticidal extract, which has a good killing effect on scab mites and Sarcoptes scabiei of rabbits and other animals. The acaricidal active ingredient of crofton weed is: 9-carbonyl-10, 11-dehydroagerophorone (euptox A). The preparation steps of a Euptox A liquid medicine include: subjecting leaf meal to methanol ultrasonic extraction; conducting ethyl acetate extraction; carrying out silica-gel column chromatography, macroporous resin column elution, and repeated silica-gel column chromatography to obtain euptox A; and conducting dissolving with a Tween-80 cosolvent to prepare euptox A containing liquid medicines of different concentrations. When the euptox A is 2mg / mL, it has a strong mite killing effect on Psoroptes cuniculi and Sarcoptes scabiei, and when the LT50 is 1.212 and 0.687h respectively, the LC50 within 2h on Psoroptes cuniculi and Sarcoptes scabiei are 0.902mg / mL and 1.068mg / mL respectively.

Description

technical field [0001] The invention relates to a high-efficiency, low-toxicity and environment-friendly plant extract for killing acarids, in particular to the preparation and application of a plant toxin for killing animal acarids. Background technique [0002] Animal acariasis is a chronic ectoparasitic disease of animals caused by mite parasites on the surface. Its pathogens are sarcoptic mites and itchy mites, which mainly occur in late autumn, winter and early spring, through direct or indirect contact between sick animals and healthy animals Infection, widely spread in cattle, sheep, horses, dogs, rabbits, chickens and other animals. It can also infect humans, and the infection and morbidity rates are relatively high in pastoral areas, farming and pastoral areas, and large-scale farms. The main manifestations of the disease in animals are loss of appetite or waste, emaciation, hair loss, etc., which affects the production performance and economic value of animals, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C49/653C07C45/78A61K31/122A61P33/14
Inventor 胡延春廖飞何洁谭辉武雷罗彪罗丽娟
Owner SICHUAN AGRI UNIV
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