Preparation method for nucleic acid vaccine based on porcine circovirus

A technology for porcine circovirus and nucleic acid vaccines, applied in the field of preparation of porcine circovirus nucleic acid vaccines, can solve problems such as increased economic losses and reproductive barriers, and achieve the effects of reduced economic losses, good immune effects, and good safety

Inactive Publication Date: 2015-02-18
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ORF1 reading frame homology of PCV1 and PCV2 is as high as 86%. At present, most reports say that PCV1 has no pathogenicity, but it has been reported that it can cause reproductive disorders. The immune program of porcine circovirus is very mature, but its However, the resulting economic loss continues to increase, which can well explain that the existence of PCV1 can promote the pathogenicity of PCV2

Method used

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  • Preparation method for nucleic acid vaccine based on porcine circovirus
  • Preparation method for nucleic acid vaccine based on porcine circovirus
  • Preparation method for nucleic acid vaccine based on porcine circovirus

Examples

Experimental program
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Embodiment Construction

[0014] Embodiments of the present invention: based on the preparation method of porcine circovirus nucleic acid vaccine:

[0015] Eukaryotic expression plasmid pEGFP-C1-PCV2-ORF1, tryptone, yeast extract, sodium chloride, endotoxin-free plasmid extraction related reagents, etc.

[0016] Including the following steps:

[0017] First, the design and synthesis of primers

[0018] Primers were obtained from GeneBank (www.ncbi.nlm.nih.gov) to obtain SVP-321 strain (accession number: EU747085), Vos-2689006 strain (accession number: EU545551), P701-1 strain (accession number: FJ905468), PCV2 - HZ0201 strain (accession number: AY-188355) and PCV2herd D strain (accession number: EU136720) sequence, with Bioedit software comparison to select the conserved sequence after Primer Premier 5 software design porcine circovirus type Ⅱ ORF1 gene (with the sequence The nucleotide sequence shown in SEQ ID NO: 1 in the list) universal primer; the sequence of the upstream primer is Primer F 5'-CG...

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Abstract

The invention discloses a preparation method for a nucleic acid vaccine based on porcine circovirus (PCV). According to the nucleic acid vaccine, a condition that homology of ORF1 reading frames of PCV1 and PCV2 reaches up to 86% is researched aiming at ORF1 genes, high levels of corresponding antibodies in animal bodies can be produced through stimulation, and high specificity is represented; aiming at porcine infection and spread caused by PCV1 and PCV2, the nucleic acid vaccine can realize a prevention and control effect on the two virus (namely PCV1 and PCV2) through the similar reading frames of the two virus so as to realize the safe and effective effect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method based on porcine circovirus nucleic acid vaccine. Background technique [0002] Porcine circovirus (porcine circovirus, PCV) is a member of the genus Circovirus in the Circoviridae family (Circoviridae), a single-stranded circular DNA virus, and one of the smallest known animal viruses. According to the differences in genome and antigenicity, it is divided into two genotypes (or serotypes) of PCV1 and PCV2. PCV1 has not been found to be pathogenic so far, and PCV2 has been identified as the cause of postweaning multisystemic wasting syndrome (Postweaning multisystemic wasting syndrome) in piglets. syndrome, PMWS) is the main pathogen. PCV2 is often co-infected with parvovirus (PPV), porcine reproductive and respiratory syndrome virus (PRRSV), mycoplasma hyopneumoniae, and pseudorabies virus (PRV), resulting in immune failure of pigs. The genomes of PCV1 and PCV...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K39/12A61P31/20C12N15/85C12N15/66C12R1/93
Inventor 王开功郭颖初周碧君文明程振涛
Owner GUIZHOU UNIV
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