Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of Burkholderia and its application in the prevention and treatment of Panax notoginseng soil-borne diseases

A Burkholderia and Panax notoginseng soil technology, applied to Burkholderia strains and its application in the prevention and treatment of Panax notoginseng soil-borne diseases, can solve the problems of high cost, unsatisfactory effect, allergic reaction, etc., and achieve high safety sexual effect

Active Publication Date: 2017-02-15
YUNNAN NORMAL UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Root rot is caused by the imbalance of microorganisms in the soil, a large number of pathogens and fungi infecting the roots of Panax notoginseng plants. The imbalance of microorganisms in the soil is an important factor leading to continuous cropping obstacles of Panax notoginseng. Currently, the commonly used treatment methods are mainly the application of fungicides. However, the effect is becoming less and less ideal, and the method of using high-efficiency fumigants for soil killing treatment, and then supplementing probiotics to adjust the microbial flora to activate and strengthen the soil is relatively expensive and the effect is not ideal.
[0004] Burkholderia is a kind of Pseudomonas, which is widely distributed in the natural environment. Different strains have the same phenotype, but different genotypes. Some types of Burkholderia can be used in agriculture for biodegradation, biological control and Rhizosphere microorganisms that promote plant growth, but some are plant pathogenic bacteria and even human condition pathogenic bacteria, and because Burkholderia has strong antibiotic resistance and high motility, therefore, in agriculture The application of Burkholderia has certain potential risks, and the selection of strains needs to be very cautious
Chinese patent 201010290583.8 discloses a Burkholderia oryzae rhizosphere. This bacterial strain has a very good effect on the prevention and treatment of rice sheath blight, but when the bacterial suspension of this bacterial strain is used to inoculate tobacco leaves, it will cause typical symptoms within 24 hours. Allergic reactions, therefore, if Burkholderia is to be used to solve soil-borne diseases of different crops, how to isolate and purify the most suitable Burkholderia strain is very important
[0005] At present, there have been many studies on the obstacles of continuous cropping of Panax notoginseng, but the use of probiotics in the rhizosphere soil of Panax notoginseng, especially Burkholderia, can inhibit the occurrence of soil-borne diseases of Panax notoginseng, thereby solving the problem of continuous cropping of Panax notoginseng to a certain extent. Barrier research has not yet been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of Burkholderia and its application in the prevention and treatment of Panax notoginseng soil-borne diseases
  • A kind of Burkholderia and its application in the prevention and treatment of Panax notoginseng soil-borne diseases
  • A kind of Burkholderia and its application in the prevention and treatment of Panax notoginseng soil-borne diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The rhizosphere soil of Panax notoginseng in a field of Panax notoginseng in Wenshan area, Yunnan Province was collected for related experiments. The specific steps are as follows:

[0032] (1) Soil sample treatment: prepare a sterilized Erlenmeyer flask, pure water, and glass beads for 1 hour. Put the glass beads into an Erlenmeyer flask filled with 20ml of sterile water, and take 2.0g of fresh soil samples into the Erlenmeyer flask, mix well, and shake at 150r / min for 45min at room temperature.

[0033] (2) Coating: Take 1 mL of the suspension in (1) and add 9.0 mL of sterile water, and serially dilute 3 times to obtain a dilution of 10 -4 Then take 120 μL of the sample solution and evenly spread it on the separation medium (beef extract peptone), and do three repetitions.

[0034] (3) Cultivation and purification: the coated plate was cultivated at 28°C for 3 days, and then a single colony was picked on the beef extract peptone medium to draw a line on the ISP2 agar...

Embodiment 2

[0041] Antibacterial experiments were carried out using the strain with the preservation number CGMCC NO.9733: first culture this strain on a medium containing 0.8% polypeptone and 0.4% yeast extract for 2 days, then pick a single colony and transfer it to a shaker flask Cultivate it inside for 3 days; then use the water agar diffusion method to react its metabolites with plant pathogenic bacteria: Fusarium apicosum (Pathogen of Panax notoginseng), Fusarium wilt of banana, Botrytis cinerea, Tobacco red spot fungus, Black spot fungus of cabbage, Such as Figure 2-Figure 6 , found that its metabolites have a good antagonistic effect on plant pathogenic bacteria.

Embodiment 3

[0043] Culture the strain with the preservation number CGMCC NO.9733 on a medium containing 0.8% polypeptone and 0.4% yeast extract for 2 days, and prepare a glass test tube filled with sterile water; make the bacteria suspension solution, the bacterial suspension is OD 600 =1.62, then the prepared bacterial suspension was diluted 1:100 times for application. Prepare 3 replicates and a blank control, apply them to pots of Panax notoginseng grown normally under the same conditions, observe and record Panax notoginseng in 1 month, 3 months, 6 months, and 12 months As a result, it was found that after 1 month, the plants of the control group had no significant difference compared with the plants of the test group; after 3 months, there were differences between the plants of the control group and the test group, showing For: 17.96% of the plants in the control group had symptoms of yellowing and edge atrophy, while only 4.23% of the plants in the test group had such symptoms; 29....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Burkholderia arboris strain YIM AF 1514, which was preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee on September 24, 2014. The preservation number is: CGMCC NO.9733. At the same time, it discloses The bacterial suspension prepared using the bacterial strain provided by the invention. The Burkholderia strain and the bacterial suspension provided by the invention can effectively prevent soil-borne diseases in the cultivation of Panax notoginseng, reduce the incidence of root rot of Panax notoginseng, and increase the emergence rate of Panax notoginseng.

Description

technical field [0001] The invention relates to a bacterial strain, in particular to a Burkholderia strain and its application in preventing and treating soil-borne diseases of Panax notoginseng. Background technique [0002] Panax notoginseng is the main economic crop in Wenshan area of ​​Yunnan Province. It is warm and humid in nature, and its unique growth environment is easy to induce a variety of soil-borne diseases. Due to the magical medicinal effects of Panax notoginseng, the global demand for Panax notoginseng and its related products is increasing, but there are not many areas available for planting Panax notoginseng, so most areas adopt the continuous cropping method of Panax notoginseng. With the increase of Panax notoginseng planting years, the types, diseased area and severity of soil-borne diseases are increasing year by year, which seriously damages the yield and quality of Panax notoginseng. The most important disease is root rot. [0003] Root rot is cause...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/01
CPCA01N63/00A01N63/10C12N1/205C12R2001/07
Inventor 官会林陆晓菊唐蜀昆
Owner YUNNAN NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com