A kind of Burkholderia and its application in the prevention and treatment of Panax notoginseng soil-borne diseases

Abstract

The invention discloses a Burkholderia arboris strain YIM AF 1514, which was preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee on September 24, 2014. The preservation number is: CGMCC NO.9733. At the same time, it discloses The bacterial suspension prepared using the bacterial strain provided by the invention. The Burkholderia strain and the bacterial suspension provided by the invention can effectively prevent soil-borne diseases in the cultivation of Panax notoginseng, reduce the incidence of root rot of Panax notoginseng, and increase the emergence rate of Panax notoginseng.

Description

technical field [0001] The invention relates to a bacterial strain, in particular to a Burkholderia strain and its application in preventing and treating soil-borne diseases of Panax notoginseng. Background technique [0002] Panax notoginseng is the main economic crop in Wenshan area of ​​Yunnan Province. It is warm and humid in nature, and its unique growth environment is easy to induce a variety of soil-borne diseases. Due to the magical medicinal effects of Panax notoginseng, the global demand for Panax notoginseng and its related products is increasing, but there are not many areas available for planting Panax notoginseng, so most areas adopt the continuous cropping method of Panax notoginseng. With the increase of Panax notoginseng planting years, the types, diseased area and severity of soil-borne diseases are increasing year by year, which seriously damages the yield and quality of Panax notoginseng. The most important disease is root rot. [0003] Root rot is cause...

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Problems solved by technology

[0003] Root rot is caused by the imbalance of microorganisms in the soil, a large number of pathogens and fungi infecting the roots of Panax notoginseng plants. The imbalance of microorganisms in the soil is an important factor leading to continuous cropping obstacles of Panax notoginseng. Currently, the commonly used treatment methods are mainly the application of fungicides. However, the effect is becoming less and less ideal, and the method of using high-efficiency fumigants for soil killing treatment, and then supplementing probiotics to adjust the microbial flora to activate and strengthen the soil is relatively expensive and the effect is not ideal.

[0004] Burkholderia is a kind of Pseudomonas, which is widely distributed in the natural environment. Different strains have the same phenotype, but different genotypes. Some types of Burkholderia can be used in agriculture for biodegradation, biological control and Rhizosphere microorganisms that promote plant growth, but some are plant pathogenic bacteria and even human condition pathogenic bacteria, and because Burkholderia has strong antibiotic resistance and high motility, therefore, in agriculture The application of Burkholderia has certain potential risks, and the selection of strains needs to be very cautious

Chinese patent 201010290583.8 discloses a Burkholderia oryzae rhizosphere. This bacterial strain has a very good effect on the prevention and treatment of rice sheath blight, but when the bacterial suspension of this bacterial strain is used to inoculate tobacco leaves, it will cause typical symptoms within 24 hours. Allergic reactions, therefore, if Burkholderia is to be used to solve soil-borne diseases of different crops, how to isolate and purify the most suitable Burkholderia strain is very important

[0005] At present, there have been many studies on the obstacles of continuous cropping of Panax notoginseng, but the use of probiotics in the rhizosphere soil of Panax notoginseng, especially Burkholderia, can inhibit the occurrence of soil-borne diseases of Panax notoginseng, thereby solving the problem of continuous cropping of Panax notoginseng to a certain extent. Barrier research has not yet been reported

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