Application of chiral nano-selenium materials loaded with siRNA in the preparation of antitumor drugs

An anti-tumor drug, nano-selenium technology, applied in the direction of anti-tumor drugs, drug combinations, pharmaceutical formulas, etc., can solve undiscovered problems, achieve the effects of reducing efflux, convenient and quick storage and use, and increasing drug intake

Active Publication Date: 2017-11-21
JINAN UNIVERSITY
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] So far, no reports have been found about chiral nano-selenium fluorescent composite materials loaded with siRNA for the preparation of anti-tumor drugs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of chiral nano-selenium materials loaded with siRNA in the preparation of antitumor drugs
  • Application of chiral nano-selenium materials loaded with siRNA in the preparation of antitumor drugs
  • Application of chiral nano-selenium materials loaded with siRNA in the preparation of antitumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1: [(phen) 2 Ru(bpibp)Ru(phen) 2 ] 4+ -L-arginine modified nano-selenium (Ru@L-SeNPs) and ((phen) 2 Ru(bpibp)Ru(phen) 2 ] 4+ -D-arginine modified nano-selenium (Ru@D-SeNPs) preparation

[0054] (1)((phen) 2 Ru(bpibp)Ru(phen) 2 ](ClO 4 ) 4 And [(bpy) 2 Ru(bpibp)Ru(bpy) 2 ](ClO 4 ) 4 Preparation method

[0055] ①cis-[Ru(phen) 2 Cl 2 ]·2H 2 Synthesis of O

[0056] Weigh RuCl 3 ·NH 2 O (0.78g, 3mmol), o-phenanthroline (1.2g, 6mmol) and lithium chloride (0.84g, 14mmol) were put in a three-necked flask, 5mL DMSO was added, and heated to reflux at 140°C under argon protection for 8 hours. After cooling to room temperature, add 25 mL of acetone, shake well and freeze overnight. Suction filtration, washing with ice water and cold acetone for 3 times, and drying in a vacuum dryer to obtain purple-black microcrystals. The crude product was recrystallized with ethanol and water (V / V=1:1), and the yield was 0.96 g (67%, calculated based on o-phenanthroline).

[0057] cis-[Ru(bpy) ...

Embodiment 2

[0068] Example 2: [(phen) 2 Ru(bpibp)Ru(phen) 2 ] 4+ -L-cysteine ​​modified nano-selenium and [(phen) 2 Ru(bpibp)Ru(phen) 2 ] 4+ -D-cysteine ​​modified nano-selenium preparation

[0069] (1) Ruthenium complex stock solution, Na 2 SeO 3 The preparation and concentration of the solution are the same as in Example 1;

[0070] (2) Dissolve L-cysteine ​​and D-cysteine ​​in purified water to prepare a 1 mol / L solution, and store it at 4°C for later use;

[0071] (3) Take 1mL Na respectively 2 SeO 3 The solution was mixed with 8mL ultrapure water to obtain solution A; 1mL of L-cysteine ​​solution was added dropwise to solution A under constant stirring, and the pH value was maintained at 9-11 during the process; the stirring was continued for 10-30 minutes to obtain wine red Nanosol; centrifuge, discard the supernatant, wash, and dry to obtain red L-cysteine ​​modified selenium nanoparticles. The obtained nano-selenium was resuspended to obtain 10 mL of 1 mM nano-selenium solution, and 2 m...

Embodiment 3

[0073] Example 3: [(bpy) 2 Ru(bpibp)Ru(bpy) 2 ] 4+ -L-Ascorbic acid modified nano-selenium and [(bpy) 2 Ru(bpibp)Ru(bpy) 2 ] 4+ -D-ascorbic acid modified nano-selenium preparation

[0074] (1) Ruthenium complex stock solution, Na 2 SeO 3 The preparation and concentration of the solution are the same as in Example 1;

[0075] (2) Dissolve L-ascorbic acid and D-ascorbic acid in purified water to prepare a 1mol / L solution, and store it at 4°C for later use;

[0076] (3) Take 1mL Na respectively 2 SeO 3 Mix the solution with 8 mL ultrapure water to obtain solution A; add 1 mL of L-ascorbic acid solution dropwise to solution A under constant stirring, maintaining the pH value of 3 to 5 during the process; continue stirring for 10 to 30 minutes to obtain wine red nano sol; Centrifuge, discard the supernatant, wash, and dry to obtain red nano-selenium particles. Resuspend the obtained nano-selenium to obtain 10 mL of 1 mM nano-selenium solution, and add 2 mL of [(bpy) under constant stirri...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to view more

Abstract

The invention belongs to the technical field of tumor targeted imaging and discloses an application of chiral nano-selenium material supported siRNA in preparation of an antitumor drug. According to the invention, both a chiral nano-selenium composite material and the chiral nano-selenium composite material supported MDR-siRNA have tumor inhibiting activities. The functional nano-selenium prepared according to the invention is not only used as an active component for inhibiting tumors, but also used as a carrier for supporting the MDR-siRNA and has an efficient antitumor effect because of the MDR-siRNA capable of inhibiting the growth of multidrug-resistant tumor cells under a synergic effect. In addition, a modifier adopted according to the invention can be used for effectively controlling the shape and size of the nano-selenium and is beneficial to increasing of intake of the cells to the drug and reduction of excretion, so that the drug in the cells is kept at the relatively-high level. Meanwhile, the chiral nano-selenium material supported siRNA prepared according to the invention has a fluorescence characteristic, and can be used as a fluorescent nanomaterial to be applied to targeted imaging.

Description

Technical field [0001] The invention belongs to the technical field of tumor targeted imaging, and particularly relates to the application of chiral nano-selenium composite materials loaded with siRNA in the preparation of anti-tumor drugs and targeted imaging. Background technique [0002] Cancer has become the number one killer threatening human health and lives, and its danger should not be underestimated. In addition, in the past 20 years, cancer has shown a trend of younger age and higher morbidity and mortality. The development of high-efficiency, low-toxicity, broad-spectrum, and highly selective anti-cancer drugs has always been an important topic in the field of cancer treatment and the direction of the efforts of scientists. [0003] Tumors are new organisms formed by the abnormal proliferation of cells in local tissues under the action of various tumor-causing factors. They often appear as local masses. They grow vigorously and often show continuous growth. At present,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/04A61K33/24A61K48/00A61K49/00A61P35/00
Inventor 刘杰陈庆昌刘亚楠于倩倩郑文静
Owner JINAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap