Five-step method for inducing lilium sulphureum bulbil callus to re-differentiate aseptic seedlings

A technique of light yellow lily flower, callus, applied in the field of biology

Inactive Publication Date: 2015-03-11
毕节市中药研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been many studies on group cultivation of Oriental Lily, New Iron Pao Lily, Huzhou Lilium, Fine Leaf Lilium, Lanzhou Lilium, Wild Lily, etc., and different lilies have different genotypes and growth environments due to their differences. The technical conditions of different plants are quite different, especially in the process of tissue culture rapid propagation, there are great differences in the type of basic medium used and the type and concentration of exogenous plant growth regulators added. There are still few systematic studies on tissue culture and rapid propagation technology of Lilium light yellow

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0025] Pick the bulbils that grow from the base of the leaves of Lilium japonica with tweezers and put them back in a pre-prepared beaker; first peel off the small scales with tweezers on the test bench, and then place the small scales in a clean beaker. Add 2~3 drops of ordinary detergent or appropriate amount of washing powder to wash 2~3 times, then pour it on the gauze prepared in advance, tie the gauze wrapped with small scales on the faucet and rinse it with tap water for 1~3h; The explant material after washing was placed on the ultra-clean workbench, soaked in 75% alcohol for 30s, and stirred with tweezers continuously during this period, so that the alcohol could immerse the explant, then rinsed with sterile water for 3 times, and then rinsed Pour the explants into 0.1% mercuric chloride for disinfection for 8-10 minutes, wash them with sterile water for 6 times, each time for 2 minutes, and finally pour the explants into the prepared sterile petri dishes, and absorb t...

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Abstract

The invention relates to a five-step method for inducing lilium sulphureum bulbil callus to re-differentiate aseptic seedlings. According to the invention, lilium sulphureum bulbils are adopted as explants; through transition culturing, pollution-free explants are transferred to a callus culturing medium; a proliferation culturing medium is used for proliferating the callus; clustered buds are differentiated from the callus; and the clustered buds are transferred to a rooting and seedling-developing culturing medium. In each step, optimization is carried out upon different explant growth regulator types, concentrations and ratios. With the method, pollution-free explants can be obtained. Callus can be induced in 7 days, wherein an induction rate reaches 100%. The callus can be sub-cultured more than 10 times. Clustered buds can be induced in 15 days, wherein an induction rate reaches 98%. Robust aseptic seedlings with roots can be obtained in 14 days, wherein an induction rate reaches 100%. Compared to conventional propagation method of sowing, bulbil and scale cutting, and the like, the method provided by the invention has the advantages of short period, low cost, and high propagation coefficient.

Description

technical field [0001] The invention belongs to the tissue culture in the field of biotechnology, and in particular relates to a production method for inducing a five-step method to induce a lily bulbil callus to differentiate into an aseptic seedling. Background technique [0002] Lilium sulphureum Baker is a wild species in the Liliaceae Lilium genus. It is produced in Guizhou Province. It is a perennial herbaceous plant. However, due to overexploitation, it is currently endangered. Lilium flaxensis is conventionally propagated by bulbs, scale cuttings, seeds, etc., but the reproduction coefficient is low, and long-term vegetative propagation is easy to cause virus infection, which seriously affects the quality and yield. Tissue culture rapid propagation is an effective way to solve the above problems. There have been reports on the tissue culture of different lily varieties at home and abroad, but there are few reports on Lilium fruticosa. [0003] Li Dai (2012) et a...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): A01H4/00
Inventor张翔宇陈杰阮培均吉云严显进王彩云王永查钦邹涛
Owner毕节市中药研究所