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A calamus four-flavor anti-cancer extract and its preparation method and application

A technology of four kinds of calamus and extracts, which is applied in the field of anti-cancer extracts of four kinds of calamus and its preparation, can solve the problems of unresearched, in-depth and unpublished pharmacological research of four kinds of calamus.

Active Publication Date: 2018-02-02
董玉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the research on the material basis and pharmacological effects of Calamus four flavors compound is not in-depth. So far, there is no research involving the combination of chemical substance clusters and pharmacological effects through modern means, clarifying the pharmacological effects of chemical substance clusters, and then enriching effective ones. Substance clusters provide a relatively clear pharmacological basis for compound pharmacological effects, such as the anticancer active chemical substance clusters in Calamus four flavors
For example, in the Chinese patent CN102058814, although a method for extracting effective substances from the four flavors of calamus is disclosed, it has not conducted in-depth research on the four flavors of calamus, especially has not disclosed any relevant pharmacological research

Method used

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  • A calamus four-flavor anti-cancer extract and its preparation method and application
  • A calamus four-flavor anti-cancer extract and its preparation method and application
  • A calamus four-flavor anti-cancer extract and its preparation method and application

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Effect test

Embodiment 1

[0123] The preparation method of a calamus four flavors anticancer extract described in the present embodiment comprises the following steps:

[0124] (1) Take 1 kg of calamus four-flavor compound medicinal herb decoction pieces, add 10 L of 50% ethanol for reflux extraction twice, extract for 2 hours each time, and recover the solvent under reduced pressure to obtain a crude extract;

[0125] (2) Add a certain volume of water to the crude extract to disperse and dissolve, the volume ratio of the weight part of the crude extract to the dispersed solution is 1:3.5, and then use the amount to account for the four flavors of Acorus calamus AB-8 macroporous adsorption resin with 7.5 times the volume of the crude drug is used for adsorption treatment, the adsorption flow rate is controlled at 2Bv / h, the resin column diameter-to-height ratio is 1:10, and 2 times the resin volume is eluted with water for impurity removal. The impurity removal flow rate is 2BV / h;

[0126] (3) Elute 8...

Embodiment 2

[0129] The preparation method of a calamus four flavors anticancer extract described in the present embodiment comprises the following steps:

[0130] (1) Take 0.5 kg of calamus four-flavor compound medicinal herb decoction pieces, add 80% ethanol and 15 L of ultrasonic extraction for 3 times, extract for 1 hour each time, and recover the solvent under reduced pressure to obtain a crude extract;

[0131] (2) Add a certain volume of water to disperse and dissolve, the volume ratio of the weight portion of the crude extract to the dispersed solution is 1:10, and the volume ratio of the crude drug of Acorus calamus four flavors is 10 volume times. AB-8 macroporous adsorption resin is used for adsorption treatment, the adsorption flow rate is controlled to be 1Bv / h, the resin column diameter to height ratio is 1:4, and then 1 times the resin volume is eluted with water for impurity removal, and the impurity removal flow rate is 4BV / h;

[0132] (3) elution with the first eluent sol...

Embodiment 3

[0135] The preparation method of a calamus four flavors anticancer extract described in the present embodiment comprises the following steps:

[0136] (1) Take 1.5 kg of decoction pieces of calamus four-flavor compound medicinal materials, add 5 L of 40% ethanol to reflux extraction 4 times, extract for 3 hours each time, and recover the solvent under reduced pressure to obtain a crude extract;

[0137] (2) Add a certain volume of water to dissolve the crude extract, the volume ratio of the weight part of the crude extract to the dispersed solution is 1:2.5, and then take the crude drug amount of the calamus four flavors 6 times the volume of D101 macroporous adsorption resin for adsorption treatment, the adsorption flow rate is controlled to 3Bv / h, the diameter-to-height ratio of the resin column is 1:13, and 5 times the volume of the resin is eluted with water for impurity removal, and the impurity removal flow rate is controlled to be 6BV / h h;

[0138] (3) Remove impuritie...

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Abstract

The invention belongs to the technical field of medicines, and particularly relates to a four-flavored calamus anticancer extract, as well as a preparation method and an application of the four-flavored calamus anticancer extract. The preparation method comprises the following steps: (1) carrying out reflux extraction on a four-flavored calamus compound medicinal material with an alcohol, ketone or ester solvent, decompressing to recover the solvent to form a crude extract, (2) adding water into the crude extract for dissolution, carrying out adsorption treatment with macroporous adsorbent resin, eluting with water for purification, (3) eluting 5-8 times of resin volume with a first elution solution for purification, controlling a flow rate at 4-9BV / h, and (4) eluting 5-8 times of resin volume with a second elution solution, controlling the flow rate at 4-9BV / h, collecting an eluant, recovering the second elution solution, and decompressing for dryness to obtain the four-flavored calamus anticancer extract. The invention further provides the four-flavored calamus anticancer extract, as well as the preparation method and the application of the four-flavored calamus anticancer extract.

Description

technical field [0001] The invention belongs to the technical field of medicinal extracts, and in particular relates to a calamus four-flavor anti-cancer extract and its preparation method and application. Background technique [0002] The four flavors of Acorus calamus are composed of four traditional Chinese medicines: galangal, purple scorpion, woody, and calamus, which are commonly used in Mongolian medicine. Traditional Mongolian medicine theory believes that the four flavors of calamus have the functions of relieving asthma and relieving pain, and suppressing "Badaganheyi". For the treatment of diaphragm spasm, chest tightness, shortness of breath, sternocostal tingling, indigestion. [0003] Among the four flavors of calamus, galangal is the dry rhizome of the ginger plant (Alpinia officinarum Hance). It is pungent and hot in nature, and returns to the spleen and stomach meridians. It has the functions of warming the stomach and dispelling cold, eliminating food and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/9062A61P35/00A61K33/14
CPCA61K9/08A61K33/14A61K36/285A61K36/888A61K36/9062A61K2236/33A61K2236/333A61K2236/39A61K2236/51A61K2236/55A61K2300/00
Inventor 董玉
Owner 董玉