Extraction and separation of compound from Tibetan medicine Rise and its method and use

A compound, russian technology, applied in the field of phytochemistry, can solve the problem of insufficient research on the chemical components of Tibetan medicine russian, and achieve the effects of easy extraction and separation, simple method and simple separation method.

Active Publication Date: 2016-05-11
CHENGDU PUSH BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] It can be seen that the current research on the chemical components of Tibetan medicine Erse is not deep enough, and its active components need to be further clarified, and the relationship between its pharmacological activity and active components needs to be clarified

Method used

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  • Extraction and separation of compound from Tibetan medicine Rise and its method and use
  • Extraction and separation of compound from Tibetan medicine Rise and its method and use
  • Extraction and separation of compound from Tibetan medicine Rise and its method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The extraction and separation of embodiment 1 compound

[0035] Take 500g of dried leaves of Ese, crush them, add 6 times the weight concentration of 80% (W / W) ethanol, heat and reflux for extraction 5 times, combine the extracts, concentrate under reduced pressure until there is no alcohol smell, and get 700ml of concentrated extracts, add 4L of water Carry out dispersion treatment, then the aqueous dispersion is extracted 4 times respectively with equal volume ethyl acetate, n-butanol successively, obtain ethyl acetate extract 15g, n-butanol extract 8g and water phase part through recovering solvent. The n-butanol extract is completely dissolved in methanol, filtered through a 0.45 μm microporous membrane, and the filtrate is prepared and separated by high-pressure C18 reverse-phase chromatography packing, A: pure methanol B: 0.2% phosphoric acid water (V / V), A: B30:70 (V / V) is the mobile phase; the detection wavelength is 210nm, the corresponding chromatographic peak...

Embodiment 2

[0036] The extraction and separation of embodiment 2 compound

[0037]Take 500g of dried leaves of Ese, crush them, add 10 times the weight concentration of 95% (W / W) ethanol to heat and reflux for extraction 4 times, combine the extracts, concentrate under reduced pressure until there is no alcohol smell, and obtain 850ml of concentrated extracts, add 6L of water Dispersion treatment was carried out, and then the water dispersion was extracted 5 times with equal volumes of ethyl acetate and n-butanol successively, and the solvent was recovered to obtain 17 g of ethyl acetate extract, 8.5 g of n-butanol extract and the water phase. The n-butanol extract is completely dissolved in methanol, filtered through a 0.45 μm microporous membrane, and the filtrate is prepared and separated by high-pressure C18 reverse-phase chromatography packing, A: pure methanol B: 0.2% phosphoric acid water (V / V), A: B30:70 (V / V) is the mobile phase; the detection wavelength is 210nm, the correspondi...

Embodiment 3

[0038] The extraction and separation of embodiment 3 compound

[0039] Take 500g of dried leaves of Ese, crush them, add 12 times the weight concentration of 40% (W / W) ethanol to heat and reflux for extraction for 3 times, combine the extracts, concentrate under reduced pressure until there is no alcohol smell, and get 2500ml of concentrated extracts, add 15L of water Dispersion treatment was carried out, and then the aqueous dispersion was extracted three times with equal volumes of ethyl acetate and n-butanol successively, and 13.8 g of ethyl acetate extract, 7.8 g of n-butanol extract and the water phase were obtained by recovering the solvent. The n-butanol extract is completely dissolved in methanol, filtered through a 0.45 μm microporous membrane, and the filtrate is prepared and separated by high-pressure C18 reverse-phase chromatography packing, A: pure methanol B: 0.2% phosphoric acid water (V / V), A: B30:70 (V / V) is the mobile phase; the detection wavelength is 210nm,...

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Abstract

The invention provides a compound extracted and separated from Tibetan medicine malus toringoides as well as a method and an application of the compound, belonging to the technical field of plant chemistry. The compound is structurally characterized by being as shown in the specification, and the chemical name of the compound is 1,2-epoxy-1-(3,4-dyhydroxyl-pheny)-3-(2,4,6-trihydroxyl-phenyl)-propane. The extraction and separation method comprises the following steps: crushing dried malus toringoides leaves, adding ethanol, heating for reflux extraction, performing vacuum concentration till no alcohol is generated, adding a proper amount of water for dispersion, extracting by using ethyl acetate and n-butyl alcohol in sequence, recycling a solvent so as to obtain ethyl acetate extract, n-butyl alcohol extract and water phase, completely dissolving the n-butyl alcohol extract by using pure methanol, filtering by using a micro-pore filtering membrane of 0.45mu m, performing high pressure preparation separation on the filtrate by using C18 reversion phase chromatography packing, collecting corresponding chromatographic peaks, and concentrating the collecting liquid, thereby obtaining a gray powder product. The compound can be used for preparing an antibacterial medicine.

Description

technical field [0001] The invention relates to a medicinal compound and its extraction method and use, in particular to a pharmacologically active compound extracted and separated from Tibetan medicine Rise and its method and use, and belongs to the field of phytochemical technology. Background technique [0002] Russian color is a common medicinal material of the Tibetan people, derived from the dried leaves of the Rosaceae Malustoringoides (rehd) Hughes. or Malustiansitoria (Batal.) Schneid. Tibetan medical monographs "Tibetan Medicine Jingjing Materia Medica" and "Tibetan-Chinese Dictionary" record that it has the functions of protecting the liver and gallbladder, and attacking stagnation. Cook it with butter tea to treat hypertension, hyperlipidemia and some liver diseases. In recent years, only several extracts of Tibetan medicine Ese have been reported to have a certain hypoglycemic effect, such as the open literature "Comparison of hypoglycemic effects of different ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D303/14C07D301/32A61K31/336A61P31/04
CPCC07D301/32C07D303/14
Inventor 李启发夏柯文焕松郭建华刘丁
Owner CHENGDU PUSH BIOLOGICAL TECH
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