A kind of triterpenoid compound extracted from Alisma and its method and application

A technology of triterpenoids and compounds, applied in the field of triterpenoids, which can solve the problems of complex operation, low recovery rate, and low product content, and achieve the effect of simple and controllable method, short time-consuming, and clear pharmacological activity

Active Publication Date: 2020-04-14
CHENGDU PUSH BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] CN104497092A provides a method for extracting 23-acetyl alisitol C from Alisma, which mainly solves the complex operation, low product content and low recovery rate in the prior art

Method used

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  • A kind of triterpenoid compound extracted from Alisma and its method and application
  • A kind of triterpenoid compound extracted from Alisma and its method and application
  • A kind of triterpenoid compound extracted from Alisma and its method and application

Examples

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Effect test

Embodiment 1

[0048] A triterpenoid compound extracted and separated from Alisma alisma, the compound is a white powder, and the color developed by 10% sulfuric acid ethanol color reagent is purple red, the molecular weight is 512, and the molecular formula is C 32 h 48 o 5 , has the structural formula shown in (I):

[0049] The triterpenoids are extracted and separated from the dried tubers of Alisma alisma, and the specific process steps are as follows:

[0050] A. Grinding the dried tubers of Alisma alisma, heating and refluxing extraction with ethanol with a concentration of 95wt%, concentrating under reduced pressure to no alcohol, and then adding water to the concentrated extract for dispersion treatment to obtain an aqueous dispersion;

[0051] B. Extracting the aqueous dispersion with ethyl acetate, recovering the solvent to obtain the ethyl acetate extract and the aqueous phase;

[0052] C. Completely dissolve the ethyl acetate extract with methanol, then use silica gel as ...

Embodiment 2

[0055] A method for extracting and separating triterpenoids from Alisma, the specific process steps are as follows:

[0056] A. Grind the dried tubers of Alisma alisma, heat and reflux them with 95wt% ethanol which is 8 times its weight, and extract it under reflux for 3 times, each time for 2 hours. Water is added for dispersion treatment to obtain an aqueous dispersion;

[0057] B. The aqueous dispersion was extracted 3 times with an equal volume of ethyl acetate, and the solvent was recovered to obtain the ethyl acetate extract and the aqueous phase;

[0058] C. Dissolve the ethyl acetate extract completely with methanol, then use silica gel as a carrier, after drying, apply the sample by wet method, and separate by silica gel column chromatography (A: petroleum ether B: ethyl acetate, A:B =5:1 V / V is the mobile phase), collect the chromatographic solution of the target object, concentrate it to dryness under reduced pressure, dissolve it with ethyl acetate, filter, ad...

Embodiment 3

[0061] A method for extracting and separating triterpenoids from Alisma, the specific process steps are as follows:

[0062] A. Grind the dried tubers of Alisma alisma, heat and reflux extraction with 95wt% ethanol 10 times its weight, 2 hours each time, concentrate under reduced pressure until alcohol-free, and then extract the concentrated extract at a volume ratio of 1:8 Water is added for dispersion treatment to obtain an aqueous dispersion;

[0063] B. The aqueous dispersion was extracted 5 times with an equal volume of ethyl acetate, and the solvent was recovered to obtain the ethyl acetate extract and the aqueous phase;

[0064] C. Dissolve the ethyl acetate extract completely with methanol, then use silica gel as a carrier, dry it, load it by wet method, and separate it by silica gel column chromatography (A: petroleum ether B: ethyl acetate, A:B =2:1 V / V is the mobile phase), collect the chromatographic solution of the target object, concentrate it to dryness und...

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Abstract

The invention discloses a triterpenoid compound extracted and separated from rhizoma alismatis, a method for the triterpenoid compound and application thereof. A structural formula of the triterpenoidcompound is shown as a formula (I). Dry tubers of the rhizoma alismatis are used as raw materials for the triterpenoid compound and are separated and extracted by the aid of technological steps of ethanol reflux extraction, ethyl acetate extraction, silica gel column chromatographic separation, C18 reversed-phase chromatographic packing high-pressure preparation and separation and the like to obtain the triterpenoid compound. The triterpenoid compound, the method and the application have the advantages that the triterpenoid compound is a novel medicinal compound with pharmaceutical activity;excellent effects of inhibiting staphylococcus aureus can be realized by the triterpenoid compound, the triterpenoid compound can be applied to preparing antibacterial medicines, and reliable bases can be provided to preparing novel antibacterial medicines.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and in particular relates to a pharmacologically active triterpenoid extracted from Alisma alisma and its method and application. Background technique [0002] Alisma is a plant in the family Alismaceae Alisma orientalis (Sam.) Juzep. of dry tubers. Alisma has a history of more than 3,000 years of medicinal use in my country. As early as 3,000 years ago, in the collection of poems "The Book of Songs", the third paragraph of "Fenjujia" wrote: "He has a song, and his words are like his, and his son is as beautiful as jade." According to Lu Gong's "Poetry "Shu" comment: "Speaking of picking its scorpion, scorpion, this Alisma is also. Its leaves are as big as plantains, and its taste is similar. People in Xuzhou and Guangling eat it." Modern scholars Zhu Minche, Zhao Jun, etc. "The Book of Songs" Annotation "Translated "Yan Caiqi Qi" into modern Chinese "I put Alisma Alisma in a basket." C...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J17/00A61K31/58A61P31/04
CPCC07J17/00
Inventor 李波郑皓元李启发王晨曦张巨琼张佳卉徐仕银刘丁夏柯陈冲肖斌李远志
Owner CHENGDU PUSH BIOLOGICAL TECH
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