Recombination acetyl coenzyme A synthetase

Abstract

The invention discloses recombination acetyl coenzyme A synthetase. The amino acid sequence of therecombination acetyl coenzyme A synthetase is shown in SEQ ID No.2 and the nucleotide sequence of the recombination acetyl coenzyme A synthetase is shown in SEQ ID NO.1.The recombination acetyl coenzyme A synthetase has the characteristics of high thermal stability and high activity; the retaining rate of enzyme activity is 59.6% after the recombination acetyl coenzyme A synthetase is treated for 30 min at the temperature of 60 DEG C and is much improved compared with constitutive enzyme; an apparent Km value of a catalyzed oleic acid acetylation reaction is 1.2*10<-5>M; the thermal stability of enzyme in a kit can be improved, and temperature influences can be avoided or reduced in the transportation and utilization processes of the kit.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a recombinant acetyl-CoA synthetase and its application in a kit. Background technique

[0002] Free fatty acids (NEFA) refer to non-esterified fatty acids carried and transported by albumin in blood lipids. The content of NEFA in normal human plasma is very small, ranging from 50 to 1200 μmol / L, accounting for only 5% to 10% of the total fatty acids in plasma. The range is large. Free fatty acids not only participate in the composition and energy metabolism of the body, but also participate in the adjustment of many physiological functions of the body, such as the synthesis of many physiological regulatory substances.

[0003] The abnormality of NEFA has important diagnostic value in the detection of many diseases. Its increase is often used in the diagnosis of diabetes, acromegaly, hyperthyroidism, hepatitis, etc., and its decrease is often used in the diagnosis of hyp...

Images