Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of biological indicator method for the efficacy of copper sulfate in aquaculture ponds by using wheat ear fish

A technology of wheat ear fish and copper sulfate, applied in the direction of biological testing, material inspection products, etc., can solve the problems of unrealistic measurement of drug concentration, insufficient or excessive therapeutic concentration, and difficulty in grasping effective concentration, so as to achieve wide and easy source of indicator organisms Obtained, easy to raise and manage artificially

Active Publication Date: 2017-01-18
GUANGXI UNIVERSITY OF TECHNOLOGY
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because copper sulfate is easily affected by organic matter, suspended matter, total alkalinity, water temperature, pH, etc. in the water body, the environment of each pond is different, and the effective concentration is difficult to grasp. It is unrealistic to measure the drug concentration with precision instruments, and there is no rapid detection at present. Farmers usually affect production and breeding due to insufficient or excessive treatment concentration, resulting in delayed disease or toxic side effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of biological indicator method for the efficacy of copper sulfate in aquaculture ponds by using wheat ear fish
  • A kind of biological indicator method for the efficacy of copper sulfate in aquaculture ponds by using wheat ear fish
  • A kind of biological indicator method for the efficacy of copper sulfate in aquaculture ponds by using wheat ear fish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: In the laboratory environment, the water temperature is 23.0±0.5°C, the pH value is 7.3±0.2, and CaCO 3 The total alkalinity is 145±21mg / L, as CaCO 3 Under the total hardness of 88±14mg / L, the acute toxicity test was designed.

[0026] Test method: Design the concentration of copper sulfate crystals as 0.07, 0.15, 0.30, 0.60, 1.20 mg / L in the test water body, put 10 healthy ear carp into the test water, observe every 2 hours, and observe continuously for 24 hours, during which the abnormal behavior and average mortality rate will be counted. The experiment consisted of 3 repetitions and 1 blank.

[0027] Test results: The 0.07 concentration treatment group had no abnormalities within 24 hours; the 0.15mg / L concentration treatment group had reactions such as darkening of body color within 4 hours, and the average mortality rate at 4h, 8h, 16h, and 24h was 0%, 0%, respectively. 6.67%, 13.33%; in the 0.30mg / L concentration treatment group, within 4 hours, some...

Embodiment 2

[0028] Example 2: In the laboratory environment, the water temperature is 23.0±0.5°C, the pH value is 7.3±0.2, and CaCO 3 The total alkalinity is 145±21mg / L, as CaCO 3 Under the total hardness of 88±14mg / L, the drug toxicity test of wheat ear fish was designed in different time periods.

[0029] Test method: design copper sulfate crystal concentration as 0.30, 0.60mg / L test water 2 groups, start counting from the concentration time, according to 0h, 1h, 2h, 4h, 8h, 16h, put 10 healthy wheat ear fish, Observe every 2 hours, and observe continuously for 24 hours (calculated after the oyster fish is put in), during which the 24-hour average mortality rate is counted. Three replicates and one blank were designed for each group of experiments.

[0030]Test results: In the 0.30mg / L concentration treatment group, the 24h average mortality rate of the 0.30mg / L concentration group was 33.33%. The 24h average mortality rate of putting into the ear fish after 16h is 13.33%, and the 24...

Embodiment 3

[0031] Example 3: In the laboratory environment, the water temperature is 23.0±0.5°C, the pH value is 7.3±0.2, and CaCO 3 The total alkalinity is 145±21mg / L, as CaCO 3 Under the total hardness of 88±14mg / L, different fish drug toxicity tests were designed.

[0032] Test method: design copper sulfate crystal concentration as 0.30, 0.60, 1.20mg / L test water body 3 groups, put different healthy fishes (size 4.82±0.74cm), zebrafish (size 3.44±0.67cm), squid Silverfish (size 3.77±0.36cm), koi (size 12.37±1.22cm), tilapia (size 6.79±0.59), silver carp (size 15.74±1.09), grass carp (size 11.68±0.99), yellow catfish (size Specification 8.26±0.71) 10 tails each, during which the 24h average mortality rate and 96h average mortality rate were counted. Three replicates and one blank were designed for each group of experiments.

[0033] Test results: drug concentration gradient drug efficacy shows (see Table 1), under the concentration of 0.30, 0.60, 1.20mg / L copper sulfate crystals of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
hardnessaaaaaaaaaa
Login to View More

Abstract

The invention discloses a biological indicator method using pseudorasbora parva for indication of drug effect of copper sulfate in a culture pond, and belongs to the technical field of aquaculture disease control, the pseudorasbora parva is used as a biological indicator for indication of drug effect situation of the copper sulfate in the culture pond using a copper sulfate-containing reagent by observation and statistics of abnormal behavior and mortality of the pseudorasbora parva, so that rational drug use and production can be guided. The biological indicator method is simple and practical, low in price, fast and accurate, and easy to produce and manage.

Description

technical field [0001] The invention relates to the technical field of aquaculture disease prevention and control, and relates to a biological indicator method for the efficacy of copper sulfate in aquaculture ponds by using indicator organisms, in particular to a method of using the earfish as an indicator organism, according to the acute toxicity of the earfish to copper sulfate Test characteristics, a method for biological indication of the efficacy of copper sulfate. Background technique [0002] Copper sulfate is widely used as an insecticide, algae remover and disinfectant in aquaculture in my country, and is favored by farmers because of its obvious effect and low price. However, in actual production, for farmed fish with strong tolerance to copper sulfate, it is usually necessary to maintain a drug concentration for a certain period of time to achieve the purpose of disease control. The effective concentration of disease repellent is usually 0.5-0.7mg / L. Because co...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50
CPCG01N33/50
Inventor 罗福广黎娅黄杰杨军易弋伍时华
Owner GUANGXI UNIVERSITY OF TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com