Primers, probes and kits for detecting human egfr gene mutations

Abstract

The invention belongs to the field of molecular biology and particularly relates to primers, probes and a kit for detecting human EGFR gene mutations. The invention provides 7 groups of primers and probes, which can accurately detect 29 types of common human EGFR gene mutations. The kit adopting the primers and the probes has high sensitivity, can detect 1% of micro mutation templates under the background of 20 ng of wild human genomes and reduces false-negative results. Besides, the kit is simple and convenient to operate, has high controllability, can be used for detecting mass samples and is favorable for clinical operation.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a primer, a probe and a kit for detecting human EGFR gene mutation. Background technique [0002] The human epidermal growth factor receptor (EGFR) gene is located in the 7p12-14 region of the short arm of human chromosome 7 and consists of 28 exons. The protein belongs to the receptor tyrosine kinase (TKI) family and exists in all normal epithelial and some cells of mesenchymal origin. EGFR can activate tyrosine kinases, thereby opening downstream signaling pathways and ultimately mediating a series of processes such as cell differentiation, survival, migration, invasion, adhesion, and cell damage repair, playing an important role in the malignant growth of tumors. Therefore, EGFR has become an important target for tumor targeted therapy research. [0003] At present, clinical EGFR targeted therapy is mainly based on two mechanisms: small molecule tyrosine kinase i...

AI Technical Summary

Problems solved by technology

The cost of this method is low, but the operation is time-consuming, and it has two obvious disadvantages: first, the sensitivity is low, and the mutation gene abundance generally needs to reach more than 10% to be accurately detected, and false negatives may occur for samples less than 10%. result

Second, due to the subsequent need to process the PCR product, contamination is prone to occur, resulting in false positive results

Images