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Application of artesunate in preparing medicine for treating and preventing central nerve injury

A technology of artesunate and central nervous system, which is applied in the application field of medicine to achieve the effect of promoting proliferation

Inactive Publication Date: 2015-04-22
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, no reports on the effect of the drug on NSCs have been published.

Method used

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  • Application of artesunate in preparing medicine for treating and preventing central nerve injury
  • Application of artesunate in preparing medicine for treating and preventing central nerve injury
  • Application of artesunate in preparing medicine for treating and preventing central nerve injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Effect of Artesunate on the Proliferation of Neural Stem Cells in Vitro

[0022] Pregnant 14.5d SD rats were selected, anesthetized intraperitoneally with 5% chloral hydrate, the peritoneum was opened, the embryos were taken out, the scalp, skull and dura mater were removed, and the rat brain was peeled off. Under a dissecting microscope, the olfactory bulb was taken as the center of the sagittal longitudinal section, and the subependymal area was taken. Add proliferation medium (DMEM / F12, bFGF 20ng / mL, EGF 20ng / mL, 2% B27), filter through a 200-mesh filter, count cells after trypan blue staining, and adjust the number of cells to 1×10 6 / ml, transferred to a culture bottle, placed in a 5% CO2, 37°C constant temperature incubator for culture, and passaged once every three days.

[0023] Take NSCs in the logarithmic phase, digest with 0.25% EDTA trypsin for 2 minutes, and stop the digestion with 10% DMEM / F12, adjust the number of cells to 8000 / 100 μL, inoculat...

Embodiment 2

[0025] Example 2 Effect of Artesunate on Ischemic Foci in Middle Cerebral Artery Occlusion Model Rats

[0026] The rat model of MCAO was established by the rat middle cerebral artery suture method. Experimental animals were given general anesthesia by intraperitoneal injection of 5% chloral hydrate, and the right common carotid artery (CCA) and carotid bifurcation were exposed and separated through an anterior median incision. Through the small opening, insert the embolus into the common carotid artery and push it forward to the bifurcation of the middle cerebral artery to block the blood flow of the middle cerebral artery. A control group (intraperitoneal injection of the same amount of normal saline after operation) and an experimental group (intraperitoneal injection of artesunate aqueous solution 100 mg / kg after operation) were set up, with six animals in each group. 72 hours later, the small animal 7.0T head MR examination was performed, and the examination results were ...

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Abstract

The invention provides an application of artesunate in preparing medicine for treating and preventing central nerve injury. The artesunate has the facilitating function on the multiplication of neural stem cells cultured in a vitro, meanwhile infarct lesions of a big mouse with cerebral infarction is effectively reduced, and the neurologic impairment caused by cerebral apoplexy is improved. The artesunate is safe and free of obvious toxic and side effects.

Description

technical field [0001] The invention relates to a new application of artesunate, in particular to the application of artesunate in the preparation of medicines for treating and preventing central nervous system damage. Background technique [0002] Cerebrovascular disease is the biggest cause of death and disability that threatens the health of middle-aged and elderly people in my country. The existing treatments for it do not bring satisfactory prognosis. Neural stem cells (Neural Stem Cells, NSCs) mainly exist in the subependymal area of ​​the lateral ventricle wall and the dentate gyrus area of ​​the hippocampus. They are a group of special cells that can self-renew, maintain stemness, and differentiate into functional neurons. [0003] Artesunate (Artesunate, ART) is a water-soluble derivative of the semiterpene lactone compound artemisinin, which has high-efficiency, low-toxicity antimalarial effect, easy to pass through the blood-brain barrier, and is widely used in c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/357A61P9/10A61P25/28A61P7/02A61P25/00
CPCA61K31/357
Inventor 葛红飞谭亮曹鎏卢宗亮陈前伟唐俊左世伦刘昕冯华
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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