Outdoor icerya purchasi simulation biological activity assay and pesticide effectiveness evaluation method and application thereof

Abstract

The invention discloses an outdoor icerya purchasi simulation biological activity assay and pesticide effectiveness evaluation method. The method includes the steps that natural inoculation and feeding are conducted on tested insects; supplied test fungicides are prepared into liquid of a set concentration; branches with insects of potted nursery stock are integrally soaked into the prepared liquid, and then the soaked branches with insects are enveloped by a thin and soft gauze element; the potted nursery stock which is soaked with pesticide is placed in a phytotron with the light dark period being L:D=14:10 h, the temperature being 25+/-1 DEG C and the relative humidity being 75% to be fed; the death and survival condition of the tested icerya purchasi is examined after the icerya purchasi is fed for 24 hours, and the death number and the survival number are recorded; a living body plant is used for cultivating the tested insects which are soaked with the liquid, the food freshness of the icerya purchasi is guaranteed, sufficient nutrients are provided for the icerya purchasi, testing is conducted under a natural environment growth condition where the icerya purchasi is placed, the accuracy of biological assay results is effectively improved, hence, the icerya purchasi population resistance level can be known comprehensively, accurately and precisely, and a basis is provided for reasonably utilizing pesticide and making a resistance treatment plan accurately.

Description

technical field [0001] The invention relates to the field of plant insect pest prevention and control, in particular to a method for measuring biological activity and drug efficacy of cotton scales in a simulated outdoor environment and an application thereof. Background technique [0002] Cottonia genus Arthropoda, class Insecta, order Homoptera, Coccidae, genus Cottonia. Cotton scales harm plants such as Rutaceae, Fabaceae, Compositae, Rosaceae and Solanaceae. Sucking tree sap can induce soot disease, causing fallen leaves, dead shoots, and weakened trees. The first generation of adults of Cottonococcus occurs in May-June, the second generation in late July to early August, and the third generation in October. In Chongqing, 3-4 generations occur a year. 4, 7-8, 10-11 are the most female adults. Most of the female adults are concentrated in one place, and the end of the abdomen secretes white cotton-like wax, and lays eggs while secreting wax filaments. The spawning pe...

AI Technical Summary

Problems solved by technology

Indoor bioassay is a more comprehensive and accurate means of understanding the resistance level of insect field populations, and it is also a more traditional method of understanding resistance. Various bioassay methods are widely used at present, such as soaking seedlings with leaves. , drip method, the damage to the tested insects is large, and the control mortality rate is large, which affects the accuracy of the bioassay results.

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