Application of degalactotigonin and derivative thereof in preparation of broad-spectrum antitumor medicine

A technology of removing galactosides and anti-tumor drugs, applied in the field of medicine, can solve the problems of large side effects, ineffective tumors, high toxicity, etc., and achieve the effects of easy extraction and separation, broad-spectrum anti-tumor, and stable properties

Inactive Publication Date: 2015-04-29
GUIZHOU INST OF BIOLOGY
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing treatments often have the disadvantage of large side effects
Most of the existing chemotherapy drugs have the disadvantages of high toxicity and great damage to healt

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of degalactotigonin and derivative thereof in preparation of broad-spectrum antitumor medicine
  • Application of degalactotigonin and derivative thereof in preparation of broad-spectrum antitumor medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The preparation of embodiment 1 degalactose substitute saponin

[0018] Crush and dry 1 kg of whole herb with 5 times the volume of 70% ethanol. After the extract is dried, extract it with 2 times the volume of n-butanol, and then use chloroform:methanol=1: 1 ratio for elution, the amount of the solvent used is twice the amount of the extraction solution, and then Sephadex-LH20 and preparative liquid phase for purification and recrystallization to obtain desgalactotigonin.

Embodiment 2

[0019] The preparation of embodiment 2 de-galactose substitute saponins

[0020] Crush and dry 1 kg of the whole herb with 10 times the volume of 50% ethanol. After the extract is dried, extract it with 5 times the volume of n-butanol, and then use chloroform:methanol=1: 1 ratio for elution, the amount of the solvent used is 4 times the amount of the extraction solution, and then Sephadex-LH20 and preparative liquid phase for purification and recrystallization to obtain desgalactotigonin.

Embodiment 3

[0021] The preparation of embodiment 3 degalactose substitute saponins

[0022] Crush and dry 1 kg of the whole herb with 8 times the volume of 60% ethanol. After the extract is dried, extract it with 3 times the volume of n-butanol, and then use chloroform:methanol=1: 1 ratio for elution, the amount of the solvent used is 3 times the amount of the extraction solution, and then Sephadex-LH20 and preparative liquid phase for purification and recrystallization to obtain desgalactotigonin.

[0023] Determination of anti-tumor effect of desgalactotigonin:

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Apoptosis rateaaaaaaaaaa
Login to view more

Abstract

The invention relates to application of degalactotigonin and a derivative thereof in preparation of an antitumor medicine. The nightshade Solanum Linn is extracted and separated from the compound. A pharmacological experiment proves that the degalactotigonin has high inhibition rate on tumor cells at a certain concentration; the anti-patent activity of degalactotigonin is obviously higher than that of a positive medicine 10-hydroxycamptothecine; the desgalactotigonin has relatively low toxicity on normal cells, and can be used for treating tumor diseases such as liver cancer, lung cancer, gastric cancer, colon cancer, breast cancer, ovarian cancer, oral cancer, esophagus cancer, bile duct cancer and leukemia. The invention further discloses a method for extracting degalactotigonin from plants. The method is simple and feasible, and has a good industrial prospect.

Description

technical field [0001] The invention relates to the application of desgalactotigonin and its derivatives in the preparation of spectrum antitumor drugs, and belongs to the technical field of medicine. Background technique [0002] Tumor is a new organism formed by the body under the action of various carcinogenic factors, a certain cell in the local tissue loses its normal regulation of its growth at the gene level, resulting in abnormal clonal proliferation. Tumor is a disease that seriously endangers human health. Its death rate ranks second in many countries, second only to cardiovascular diseases. The methods usually used to treat tumors are surgery, chemotherapy and radiotherapy. Existing treatment methods often have the disadvantage of great side effects. Most of the existing chemotherapeutic drugs have the disadvantages of high toxicity and great damage to healthy human cells. At the same time, due to the drug resistance of tumor cells and other reasons, the existi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K31/7048A61P35/00A61P35/02
CPCA61K31/7048
Inventor 贾强
Owner GUIZHOU INST OF BIOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap