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A kind of preparation method of natural lysophosphatidylcholine

A phosphatidylcholine, a natural technology, applied in the field of preparation of natural lysophosphatidylcholine, can solve the unfavorable popularization and application of natural lysophosphatidylcholine, high price, long delivery cycle and other problems, to reduce over-reliance, The production is easy to operate and the effect of large processing capacity

Active Publication Date: 2016-08-24
GUANGZHOU HANFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Constrained by technology, domestic natural lysophosphatidylcholine mainly relies on imports, which is not only expensive, but also has a long delivery cycle, and is not conducive to the popularization and application of natural lysophosphatidylcholine products in the market

Method used

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  • A kind of preparation method of natural lysophosphatidylcholine
  • A kind of preparation method of natural lysophosphatidylcholine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Get 1 kilogram of soybean phospholipid raw material, add chloroform to dissolve and be made into raw material liquid, fill 10 kilograms of silica gels and carry out column chromatography, elute 18 liters with eluent (chloroform 52.8%, ethanol 44.2%, water 3.0%) for the first time, For the second time, 60 liters of eluent are collected with eluent (dichloromethane 45.3%, ethanol 54.0%, water 2.5%) to elute, and after vacuum concentration, freeze-drying obtains the lysophosphatidylcholine product, and the LPC transfer rate is 70.3%, the purity of lysophosphatidylcholine is 99.5%, phosphatidylcholine is not detected, phosphatidylethanolamine is not detected, triglyceride, free fatty acid all meet the requirements, acid value is 0.6, peroxide value is not detected, The methoxyaniline value was 3.8.

Embodiment 2

[0031] Get 10 kilograms of soybean lecithin raw materials, add dichloroethane to dissolve and be made into raw material liquid, pack 70 kilograms of diol-based silica gels and carry out column chromatography, first time with eluent (dichloroethane 55.0%, isopropanol 42.0%) %, water 3.0%) to elute 180 liters, and then elute 560 liters with eluent (chloroform 43.0%, propanol 54.0%, water 3.0%) for the second time to collect the eluate, concentrate in vacuo, and freeze-dry to obtain hemolytic Phosphatidylcholine products, LPC transfer rate is 80.9%, lysophosphatidylcholine purity is 99.1%, phosphatidylcholine is 0.2%, phosphatidylethanolamine is not detected, triglyceride, free fatty acid all meet the requirements, acid value 0.5, the peroxide value was not detected, and the methoxyaniline value was 2.9.

Embodiment 3

[0033] Get 20 kilograms of soybean phospholipid raw materials, add dichloromethane to dissolve and make raw material solution, fill 100 kilograms of silica gel and carry out column chromatography, wash with eluent (dichloromethane 60.0%, ethanol 38.5%, water 1.5%) for the first time Take off 200 liters, and then elute 400 liters of eluent with eluent (chloroform 35.0%, n-butanol 59.0%, water 6.0%) for the second time to collect the eluate, concentrate in vacuo, and freeze-dry to obtain the lysophosphatidylcholine product. The transfer rate of LPC is 83.3%, the purity of lysophosphatidylcholine is 99.4%, phosphatidylcholine is 0.3%, phosphatidylethanolamine is not detected, triglyceride and free fatty acid all meet the requirements, the acid value is 0.7, and the peroxide value is not It was detected that the value of methoxyaniline was 4.0.

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Abstract

The invention discloses a preparation method of natural LPC (lysophosphatidylcholine). The method comprises steps as follows: a phospholipid coming from soybeans or yolks is taken as a raw material, elution is performed twice with a ternary mixed solvent consisting of a polyhalogenated alkane, low-carbon alcohol and water through column chromatography, drying is performed, and the natural LPC product is obtained. The product obtained with the method is high in LPC content (98.0-99.9% with HPLC (high performance liquid chromatography)), low in impurity content (the phosphatidylcholine content is lower than 1.0%, the phosphatidyl ethanolamine content is lower than 1.0%, the free fatty acid content is lower than 0.2%, and the triglyceride content is lower than 0.2%) and low in oxidative index (the acid value is lower than 1.0, the peroxide value is lower than 1.0, and the anisidine value is lower than 5.0) and meets the quality requirement of the medical phospholipid.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical engineering, and in particular relates to a preparation method of natural lysophosphatidylcholine. Background technique [0002] Lysophosphatidylcholine (lysophosphatidylcholine referred to as LPC) is an excellent emulsifier with better wetting ability, emulsifying ability, emulsifying stability and other properties than ordinary phospholipids, and has antioxidant and antibacterial properties at the same time. and other fields are widely used. In the field of medicine, high-purity lysophosphatidylcholine (LPC>98%) is mainly used as an emulsifier for liposomes. Its emulsifying effect is better than that of phosphatidylcholine, and it is a key adjuvant for new targeted preparations. High-purity lysophosphatidylcholine, as the starting material for the synthesis of phospholipids, has broad application prospects. At present, the main control indicators of medicinal phospholipids recorded i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07F9/10
Inventor 许文东唐顺之李继荣关伟键袁诚蔡鸿飞杨玉琼谭巧君牟肖男李金华王小妹梁北梅
Owner GUANGZHOU HANFANG PHARMA
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