Method for synthesizing gold nanoparticles by using pycnoporus sanguineu organisms and application of method for synthesizing gold nanoparticles by using pycnoporus sanguineu organism

A technology of gold nanoparticles and Microporus hemoglobinum, which is applied in the field of microbial synthesis of gold nanoparticles, can solve the problems of limiting the application of gold nanoparticles, high cost, high energy consumption, etc.

Active Publication Date: 2015-04-29
SOUTH CHINA UNIV OF TECH
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  • Abstract
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  • Claims
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Problems solved by technology

[0003] Traditional gold nanoparticle material synthesis methods mainly include physical methods, chemical methods, and physicochemical methods. These methods usually have strict requirements on reaction conditions (such as high temperature or high pressure), resulting i

Method used

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  • Method for synthesizing gold nanoparticles by using pycnoporus sanguineu organisms and application of method for synthesizing gold nanoparticles by using pycnoporus sanguineu organism
  • Method for synthesizing gold nanoparticles by using pycnoporus sanguineu organisms and application of method for synthesizing gold nanoparticles by using pycnoporus sanguineu organism
  • Method for synthesizing gold nanoparticles by using pycnoporus sanguineu organisms and application of method for synthesizing gold nanoparticles by using pycnoporus sanguineu organism

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Embodiment 1

[0027] Pycnoporus sanguineus cultured to the stationary phase were collected by centrifugation at 10,000 rpm, 4°C, and 20 min; washed repeatedly with sterile water to remove residual medium components that might adhere. Weigh 10g (wet weight) of microorganisms into a centrifuge tube, crush at 90W, 4s / 4s for 10min, centrifuge at 11000rpm, 4°C, 20min to remove cell debris, and dilute the supernatant to 50ml, which is called intracellular extract. Take 10, 20, 40 and 80ml intracellular extracts and mix them with chloroauric acid stock solution respectively. The total volume of the mixed solution is 100ml, and the final gold ion concentration is 1.0mM. Shake at 30°C and 165rpm for 24h. First, the color of the mixed solution gradually changed from light yellow to purple. Second, the formation of gold nanoparticles was further verified by UV-vis spectroscopy, XRD, and TEM. The characterization results are as follows Figure 1-3 shown.

Embodiment 2

[0029] The steps of this embodiment are substantially the same as those of Example 1, except that 80ml intracellular extract is mixed with different volumes of chloroauric acid solution, the final gold ion concentration is 0.5, 1.0, 1.5 and 2.0mM respectively, and the mixed solution is mixed with sterile The volume of water was adjusted to 100ml, and the TEM characterization results were as follows Figure 4 shown.

Embodiment 3

[0031] The steps of this embodiment are substantially the same as in Example 1, except that 80 ml of the intracellular extract is mixed with a certain volume of chloroauric acid solution, the final gold ion concentration is 1.0 mM, and then the pH of the solution is adjusted with 0.1 M HCl or NaOH The values ​​are 2.0, 4.0, 6.0, 8.0, 10.0 and 12.0 respectively, and the TEM characterization results are as follows Figure 5 shown.

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Abstract

The invention relates to a method for synthesizing gold nanoparticles by using pycnoporus sanguineus and an application of the method for synthesizing gold nanoparticles by using pycnoporus sanguineu organisms. The method comprises the following steps: (1) culturing pycnoporus sanguineus to the stationary stage, centrifugally collecting, washing by using sterilized deionized water, performing cell lysis by using a cell cracker to release intracellular organic matters, and centrifugally collecting the supernatant to obtain the intracellular extract; and (2) mixing the intracellular extract with a chloroauric acid aqueous solution to obtain a mixed solution which is subjected to shake culture to obtain a gold nanoparticle solution which has higher catalytic property. Experiments show that the gold nanoparticle solution can rapidly and effectively catalyze the degradation of nitroaniline. The method disclosed by the invention is simple and convenient to operate, and is economic, effective, safe and environment-friendly.

Description

technical field [0001] The invention relates to a microbial synthesis method of gold nanoparticles and an application technology of a product gold nanoparticle solution as a catalyst. Background technique [0002] In recent decades, the research of nanotechnology has increasingly become the focus of scientific research worldwide, especially the synthesis technology of noble metal nanoparticle materials has received extensive attention. Among all noble metal nanoparticle materials, gold nanoparticle materials are widely used in electrical, optical, biomedical, and catalytic applications due to their high stability and biocompatibility. [0003] Traditional gold nanoparticle material synthesis methods mainly include physical methods, chemical methods, and physicochemical methods. These methods usually have strict requirements on reaction conditions (such as high temperature or high pressure), resulting in high energy consumption and high cost. And these processes may involve ...

Claims

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Application Information

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IPC IPC(8): C12P3/00B01J23/52A62D3/37C12R1/645A62D101/26
Inventor 朱能武石超宏操艳兰吴平霄
Owner SOUTH CHINA UNIV OF TECH
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