Particle three-dimensional position nanoscale resolving power measuring method under liquid state environment

Abstract

The invention relates to the field of digital holography microscopy measuring and the field of particle position measuring and provides a technology based on coaxial digital holography microscopy and an image processing method, and multi-particle three-dimensional position nanoscale resolving power measuring under a complex liquid state environment is achieved. According to the technical scheme, a particle three-dimensional position nanoscale resolving power measuring method under the liquid state environment comprises the steps that a coaxial digital holography microscopy imaging system is used for carrying out real-time tracking on particles and recording of particle holography images; the particle holography images are preprocessed; the processed holography images are subjected to holography reconstruction; the obtained reconstruction information is subjected to deconvolution operation; a centroid method is used for obtaining the horizontal positions of the particles; through the obtained particle mass center positions, a longitudinal intensity distribution curve is obtained; and polynomial fitting is used for carrying out fitting on the curve, and the peak values are the positions of the particles in the longitudinal direction. The method is mainly used in microscopy measuring.

Description

technical field [0001] The invention relates to the field of digital holographic microscopic measurement and the field of particle position measurement. Specifically, it is a method for measuring the three-dimensional position of particles in a liquid environment at the nanoscale using digital holographic microscopic measurement technology, and particularly relates to the three-dimensional particle position in a liquid environment. Position nanoscale resolution measurement method. Background technique [0002] Particles generally refer to particles ranging in size from submicron to hundreds of microns, including biological particles such as cells and viruses, as well as non-biological particles such as polystyrene beads, magnetic beads, silicon spheres, colloids, and fluorescent particles. Micron-long rods, etc. The measurement technology of the three-dimensional position of particles in the liquid environment has always been an important subject of the measurement discipli...

AI Technical Summary

Problems solved by technology

The resolution of the Mie scattering measurement method can reach 1nm, but it needs to accurately calibrate various physical parameters, the measurement method is complex, and can only measure regular particles; while the measurement resolution of other methods is about 10nm, which cannot meet the measurement requirements

The above method cannot measure multiple particles at the same time, the measurement efficiency is low, and it cannot measure overlapping particles in the liquid environment, and its robustness is low

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