Large-scale preparation method for AA (carbon source-contained) solid culture medium

Abstract

The invention discloses a large-scale preparation method for an AA (carbon source-contained) solid culture medium, and belongs to the technical field of plant biologics. The large-scale preparation method comprises the following steps: firstly, calculating the demand amounts of 19 elements of the AA (carbon source-contained) solid culture medium after being amplified for 1000 times; accurately weighing the amplified amounts of medicines, and accurately weighing CuSO4.5H2O and CoCl2.6H2O by using a one over ten-thousand analytical balance; sequentially mixing the 19 elements according to the demand amounts, namely, firstly mixing two elements of the smallest amounts, secondly subsequently adding another element of which amount is slightly smaller than that of each of the two elements of the smallest amounts and thirdly uniformly mixing till the mixing is finished according to the rule; sealing every 250g of the solid culture medium in a fixed plastic container; unsealing to use the solid culture medium according to demands. The method can prepre the AA (carbon source-contained) solid culture medium which is amplified for 1000 times, has the characteristics of being convenient and rapid to operate, simple to use and small in errors of different batches and same batches, prevents the phenomena of large errors, deposition, mildew and the like caused by artificial operation such as calculation and weighing, and external factors such as weather, temperature and moisture when the AA (carbon source-contained) solid culture medium is prepared, and can be applied to biological technical fields such as plant tissue culture.

Description

technical field [0001] The invention discloses a large-scale production method of AA (containing carbon source) solid culture substrate, which belongs to the field of plant biotechnology. More specifically, it is a five-step process including accurate calculation, accurate weighing, mixing less and more, sealing, and unsealing, in order to obtain culture substrates with minimal errors between different batches and batches, and reduce Method of trial and error. Background technique [0002] Plant tissue culture technology or soilless culture technology usually requires the preparation of the mother liquid of the medium, that is, the preparation of a liquid that is enlarged by a certain multiple and contains various types of reagents. However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic elements, and the expansion multiples of the four categories are diffe...

AI Technical Summary

Problems solved by technology

However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic elements, and the expansion multiples of the four categories are different, resulting in inconsistent quality of medicines, and one is prone to calculation errors; Two, the manufacturer and quality of the medicine are inconsistent, even if the calculation and weighing are consistent, it will affect the test results; three, because the mother liquor is in a liquid state, at the turn of spring and summer, due to the increase in temperature and humidity, it is extremely easy to cause mother liquor infection Bacteria and mold cause different colors or precipitation in the mother liquor, which wastes raw materials; four will cause large errors due to the difference in the scale of the container (especially trace elements) and the precision of the instrument.

[0003] In view of the above-mentioned shortcomings of mother liquor preparation, the preparation of culture medium with conventional mother liquor often leads to failure or unrepeatable test results due to factors such as weighers, drugs, seasons, etc., which greatly affects the use of plant tissue culture or culture media. The process of carrying out trials of soilless cultivation technology has hit the confidence of the experimenters