Traditional Chinese medicine effective component compound for preventing and treating fatty liver
A technology of active ingredients and fatty liver, applied in the direction of active ingredients of ketones, medical preparations containing active ingredients, drug combinations, etc., can solve the problems of few researches and no involvement of compound prescriptions, and achieve significant prevention and control effects, safe and effective effects effect of substance
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[0058] Experimental scheme 2--Validation experiment of effective component formula
[0059] 1. Model Preparation
[0060] Fatty liver model of rats with high-fat diet (induced by dietary factors alone), fed with high-fat diet (10% lard + 2% cholesterol + 88% basal feed) for 12 weeks, and the feed was produced by Shanghai Slack Experimental Animal Co., Ltd.
[0061] 2. Reagents and Drugs
[0062]Free fatty acid (FFA) enzyme-linked immunosorbent assay kit was purchased from Shanghai-based Biotechnology Co., Ltd., batch number: DRY1198-201306; triglyceride (triglyceride, TG) kit was purchased from Beijing Beihua Kangtai Clinical Reagent Co., Ltd. , batch number: 006304; total cholesterol (total cholesterol, TC) kit was purchased from Beijing Beihua Kangtai Clinical Reagent Co., Ltd., batch number: 006301. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) kits were purchased from Nanjing Jiancheng Bioengineering Company, batch number: 20130724; salidrosid...
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