A kind of derivative peptide of chicken source antimicrobial peptide and its preparation method and application

A technology of antimicrobial peptides and derivative peptides, which is applied in the field of chicken-derived antimicrobial peptide derivative peptides and its preparation, can solve the problems of anti-infection treatment falling into the crisis of drug-resistant bacteria, and achieve low cytotoxicity, high application value, and simple experimental techniques Effect
CN104650208BInactive Publication Date: 2018-04-13NORTHEAST AGRICULTURAL UNIVERSITY
8 Cites 2 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NORTHEAST AGRICULTURAL UNIVERSITY
Publication Date
2018-04-13
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a derived peptide for a chicken origin antibacterial peptide as well as a preparation method and application thereof. The sequence of the derived peptide is shown as SEQ No.1. The preparation method comprises the following steps: intercepting 20 amino acids of a chicken origin AvBD4 leader peptide, replacing 5th cysteine and 6th, 7th and 12th phenylalanine by amino acid lysine with charges respectively, meanwhile, carrying out amidation on a carboxyl terminal of the antibacterial peptide, and increasing a positive charge to improve the stability of the antibacterial peptide; adopting a solid-phase chemical synthesis method to obtain crude MGW21; and purifying and identifying synthesized polypeptide by using reversed-phase high performance liquid chromatography and electrospray mass spectrometry. The invention provides the application of the antibacterial peptide in preparing a medicament for treating gram-positive bacteria or gram-negative bacteria infectious diseases. The antibacterial peptide is good in amphiphilic property; compared with an antibiotic and Melittin, the antibacterial peptide is high in antibacterial activity and low in cytotoxicity.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a chicken-derived antimicrobial peptide derivative peptide and a preparation method and application thereof. Background technique

[0002] Antimicrobial peptides (AMPs), also known as antimicrobial peptides or peptide antibiotics, are a class of small molecule polypeptides produced by specific bacterial genes and have broad-spectrum antibacterial properties. Antimicrobial peptides were first discovered from Hyatophora cecropia in the 1980s. Antimicrobial peptides usually consist of 12-50 amino acid residues, with a molecular weight of less than 10kDa. Most antimicrobial peptides contain positively charged amino acids and are amphipathic and cationic. The main biological function of antimicrobial peptides is that they can kill a variety of bacteria including Gram-negative bacteria and Gram-positive bacteria, and have the characteristics of broad antibacterial spectrum and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More