Tissue culture and rapid propagation method for lycoris albiflora

A technology of tissue culture rapid propagation and milky Lycoris, which is applied in the field of plant tissue culture, can solve the problems of not being able to harvest every year, slow reproduction speed, and low reproduction coefficient, and achieve the effect of promoting commercial production

Inactive Publication Date: 2015-06-03
朱海燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reproduction coefficient of natural bulb division and seed self-sowing is very low. For example, the bulb division method is to peel off the small bulbs that grow around the big bulbs and plant them separately. However, the small bulbs grow slowly and cannot be harvested every year. It is excavated in autumn every year, and then divided into bulbs. The propagation speed is quite slow. Most species can only produce 1-2 seed bulbs per year. The number of seed bulbs is small and limited, which is far from meeting the needs of commercial production of Lycoris.
Plant tissue culture has the characteristics of fast reproduction speed, large reproduction coefficient, and high benefit. However, the tissue culture technology of Lycoris plants is still immature, and there are still many problems to be solved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Disinfection of explants: peel off the brown outermost layer of bulbs that grow robustly and are basically the same size, rinse under running water for 1 hour, cut off the top, and soak in 75% ethanol solution for 10 seconds in an ultra-clean workbench. Rinse 3 times with sterile water, then disinfect with 0.1% mercuric acid solution containing 0.01% Tween-20 for 5 minutes, rinse 3 times with sterile water, blot dry the surface with sterile filter paper for later use.

[0019] (2) Adventitious bud induction: take the bulbs treated in step (1) and cut them into four pieces in a cross method, and then cut them into double-layer or three-layer scales with a base plate of about 1.2cm×1.0cm in size. The following method was used to inoculate into the induction medium for adventitious bud induction culture. After inoculation, it was placed under the condition of 12 hours of light per day, the light intensity was 1000lx, the culture temperature was 23°C, and the relative a...

Embodiment 2

[0024] (1) Disinfection of explants: for bulbs that grow robustly and are basically the same size, peel off the outermost brown layer, rinse under running water for 2 hours, cut off the top, and soak in 78% ethanol solution for 12 seconds in an ultra-clean workbench. Rinse with sterile water for 5 times, then disinfect with 0.1% mercuric acid solution containing 0.01% Tween-20 for 7 minutes, rinse with sterile water for 5 times, dry the surface with sterile filter paper for later use.

[0025] (2) Adventitious bud induction: take the bulbs treated in step (1) and cut them into four pieces in a cross method, and then cut them into double-layer or three-layer scales with a base plate of about 1.2cm×1.0cm in size. The following method was used to inoculate into the induction medium for adventitious bud induction culture. After inoculation, it was placed in the light for 14 hours a day, the light intensity was 1500lx, the culture temperature was 25°C, and the relative air humidity...

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Abstract

The invention discloses a tissue culture and rapid propagation method for lycoris albiflora. At present, lycoris plants in China mainly perform bulb division propagation and seed self-sowing propagation in the natural state, the propagation coefficient is low, the propagation speed is low, most bulbs can only divide 1-2 child bulbs every year, the number of bulbs is small and limited, and the requirement of commercial production of the lycoris albiflora is far from being met. In order to meet the requirement of commercial production of the lycoris albiflora, in-vitro regenerated plants of the lycoris albiflora are obtained with lycoris albiflora with basal disc bulbs used as explants through processes of adventitious bud induction, subculture, rooting, acclimatization, transplantation and the like, and a tissue culture and rapid propagation technology system for the lycoris albiflora is established to promote the commercial production of the lycoris albiflora.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of milky white Lycoris. Background technique [0002] Lycoris ( Lycoris Herb ) Amaryllidaceae perennial herb. Amaryllis plants have strong adaptability, easy planting, tolerance to drought, humidity, and barrenness, extensive management, and flowering seasons in summer and autumn when the high temperature blooms and the flowers fall. Therefore, they can be widely used in landscaping. Moreover, Lycoris "does not bloom when it leaves, and does not see leaves when it blooms." It blooms first and then leaves, or blooms after leaves wither. This is very rare and unique among flowering plants, because most species of Lycoris are native to China, so it is called "China's Tulip". In addition, Lycoris bulbs contain lycorine, lycorine, galantamine, lycorine, polyflora, dihydrolycorine, galanthine, pseudo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 朱海燕
Owner 朱海燕
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