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A peanut brady-growing rhizobia agent, its preparation method and application

A technology of bradyrhizobium and rhizobium agent, which is applied in the field of preparation of high-efficiency nitrogen-fixing peanut rhizobia agent, can solve the problem that the improvement of peanut yield has no good effect, achieve strong nodulation and nitrogen fixation performance, and improve nitrogen fixation ability , enhance the effect of disease resistance

Active Publication Date: 2018-03-27
兴邦(武汉)生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But it has no good effect on the improvement of peanut yield

Method used

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  • A peanut brady-growing rhizobia agent, its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0022] Embodiment 1: bradyrhizobium agent preparation

[0023] 1. Culture medium: first prepare a liquid medium with 10 grams of mannitol, 0.5 grams of dipotassium hydrogen phosphate, 0.2 grams of magnesium sulfate, 0.2 grams of sodium chloride, 1 gram of yeast extract, and 0.1 grams of ferric chloride , adjust the pH value to 6.5-7.2, and sterilize for 30 minutes at a temperature of 121°C and a pressure of 0.1MPa. The bradyrhizobium slant strain is inoculated in the liquid medium by using an inoculation loop, and then cultivated in a constant temperature shaker at a temperature of 25-30° C. and a rotation speed of 150-200 rpm for 8 days.

[0024] 2. For seed tank cultivation, inoculate the bacteria into the seed tank at a 10% inoculum amount, at a temperature of 25-30°C, at a ventilation rate of 0.2-0.8k / s, at a stirring speed of 150-200 rpm, and cultivate for 6 days.

[0025] 3. Fermentation tank culture, according to the inoculation amount of 10%, inoculate the cultured st...

Embodiment 2

[0028] Embodiment 2: efficacy embodiment

[0029] The medicament prepared by the method of Example 1 has an effective number of viable bacteria ≥ 500 million / ml. Use the dose of 25 ml / mu on the peanut variety Zhonghua No. 8.

[0030]In this experiment, there were 7 treatments and 4 repetitions, and a completely randomized block design was adopted. The area of ​​the residential area is 5.00 meters﹡6.00 meters=30.00 square meters.

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Abstract

The invention relates to a slow-growing nitragin for peanuts as well as a preparation method and application thereof, in particular to application of slow-growing Bradyrhizobium japonicum and Bradyrhizobium yuanmingense or compositions thereof to improvement of peanut output. The nitragin provided by the invention is strong in scaffolding nitrogen fixation performance, can be used for improving the soil, strengthening the disease resistance and stress resistance of crops and remarkably improving the peanut quality and output, and belongs to a nitrogen fertilizer-saving pollution-free microbial agent.

Description

technical field [0001] The invention relates to a high-efficiency nitrogen-fixing peanut rhizobium agent and its preparation method and application. The active ingredients in the rhizobium agent are Bradyrhizobium japonicum, Bradyrhizobium yuanmingense or a combination thereof It is a bradyrhizobium agent for peanuts. The present invention further relates to the use of Bradyrhizobium japonicum, Bradyrhizobium yuanmingense or the combination thereof to improve peanut yield. Background technique [0002] It is well known that leguminous plants, under certain conditions, can directly fix and convert nitrogen in the air into organic compounds. This immobilization ability is produced by soybean rhizobia or bradyrhizobium bacteria, which are capable of forming nodules with plant rhizomes. Thus by inoculating plants with an inoculation mixture containing a large number of Bradyrhizobium bacteria, greater nitrogen fixation capacity can be obtained. It is an important experience i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A01P21/00C12N1/20C12R1/01
CPCY02P60/21
Inventor 不公告发明人
Owner 兴邦(武汉)生物科技有限公司
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