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Plasmid containing functional gene capable of degrading benzo [ghi] perylene

A functional gene and plasmid technology, applied in the field of microbiology and molecular biology, to improve the effect of biodegradation

Inactive Publication Date: 2015-06-17
BEIJING NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is a lack of research reports on PAH degradation plasmids at home and abroad

Method used

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Examples

Experimental program
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Effect test

Embodiment

[0033] Inoculate the strain Ochrobactrum lupini DW3 preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee into the glass bottle that has been added with benzo[ghi]perylene and basal medium, trace metal liquid and vitamin c solution, and then The culture bottle was cultured for 20 days in a shaker with a culture temperature of 25-28° C. and a rotation speed of 100 r / min to obtain a cultured bacterial liquid. Centrifuge the bacterial solution at 10,000r / min for 10min, and add 10mL of NaH with a pH value of 9.0 and a concentration of 0.08M to the isolated bacterial cells. 2 PO 4 Buffer solution, vortexed for 5 minutes, then ultrasonically oscillated for 15 minutes, centrifuged at 10,000 r / min for 10 minutes, discarded the supernatant, added 7 mL of solution A and mixed well. Then add 2.2 mg of N-acetylmuramic glycan hydrolase, shake at 0-4°C for 10 minutes, then add 1.5 mL of ethoxylated sodium alkyl sulfate solution with a...

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Abstract

The invention provides a plasmid containing a functional gene capable of degrading benzo [ghi] perylene. The plasmid containing the functional gene capable of degrading benzo [ghi] perylene is obtained by extracting and purifying bacterium liquid of bacterial strain of lupin Ochrobactrum lupine DW3. The preparation method of the plasmid comprises the following steps: inoculating the lupin Ochrobactrum lupine DW3 in a glass bottle which is filled with benzo [ghi] perylene, a basal culture medium, trace metal liquid ad a vitamin C solution; culturing for 20 days to obtain bacterium liquid; extracting and purifying the bacterium liquid to obtain the plasmid containing the functional gene capable of degrading benzo [ghi] perylene. The plasmid containing the functional gene capable of degrading benzo [ghi] perylene has the ability of constructing bacterial strains with degrading function for benzo [ghi] perylene, improving the biodegradation effect of polycyclic aromatic hydrocarbon pollutants represented by the benzo [ghi] perylene and having application potential for the polycyclic aromatic hydrocarbon benzo [ghi] perylene polluted soil and water.

Description

technical field [0001] The invention belongs to the technical field of microbiology and molecular biology, and specifically relates to a plasmid containing a functional gene capable of degrading benzo[ghi]perylene. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) are a class of toxic organic pollutants containing two or more benzene rings, which have strong carcinogenic, teratogenic and mutagenic effects. PAHs can cause great harm to the ecological environment and human health through bioaccumulation and food chain transmission, which has attracted great attention from environmental scientists in various countries. The treatment technologies for polycyclic aromatic hydrocarbons in the environment mainly include physical methods, chemical methods and biological methods, among which biological methods have the advantages of simple operation, low operating costs, and no secondary pollution. In general, as the number of ben...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/10C12R1/01
Inventor 谢恩郑蕾朱宜丁爱中豆俊峰
Owner BEIJING NORMAL UNIVERSITY
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